scholarly journals Development of a method for obtaining healthy mini-tubers of potatoes of the Yubilyar variety using a humic preparation from peat

2021 ◽  
Vol 928 (1) ◽  
pp. 012009
Author(s):  
M S Romanova ◽  
E V Khaksar ◽  
N I Leonova ◽  
O O Novikov ◽  
E I Kosinova ◽  
...  
Keyword(s):  
Survival Rate ◽  
Nutrient Medium ◽  
In Vitro Cultivation ◽  
Potato Plants ◽  
Mineral Components ◽  
Ground Conditions ◽  
The Cost ◽  
Biological Fertilizer ◽  
Optimal Medium

Abstract The influence of various nutrient medium compositions on the growth of potato plants during in vitro cultivation and the possibility of using biological fertilizer from peat in different concentrations to improve the adaptation of plants to growing in ground for the development of a method for obtaining healthy mini-tubers of potatoes of the Yubilyar variety were studied. The influence of various nutrient medium compositions on the height, biomass, intensity of rhizogenesis, the number of internodes of improved potato micro-plants, as well as humic fertilizer from Humostim peat on the survival rate and height of potato plants when adapting it to ground conditions is analyzed. Taking into account the data obtained, as well as the cost of the studied variants of the medium, the optimal medium for growing potato micro-plants in laboratory conditions in vitro is a nutrient medium with a content of mineral components 1/3 from the norm. The use of humic preparation from peat Humostim in a concentration of 0.001% caused an acceleration of plant growth and an increase in their height, and this concentration is recommended for use.

scholarly journals Application of auxin-cytokinin substitute in vitro culture Solanacea crops

2020 ◽  
Vol 26 ◽  
pp. 217-221
Author(s):  
R. V. Kovbasenko ◽  
O. P. Dmitriev ◽  
T. M. Oliynik
Keyword(s):  
Agar Medium ◽  
In Vitro Cultivation ◽  
Cytokinin Activity ◽  
Ms Medium ◽  
Industrial Growth ◽  
Culture In Vitro ◽  
Potato Plants ◽  
Nutrient Agar Medium ◽  
The Cost

Aim. The purpose of our research was to establish the possibility of initiation of potato and tomato culture plants using industrial growth regulators, which are legal for use in Ukraine, which include substances with pronounced auxin-cytokinin activity. Methods. In this work, we used varieties of tomato: Khoriv, Borivsky and Bozhedar, Povin potatos. Work in culture in vitro was performed according to conventional methods. Results. A phytohormone substitute was created in a known nutrient agar medium according to Murasige-Skuga. For the in vitro cultivation of tomato and potato plants, phytohormones were replaced by solutions of Ecostim and Ecostim 1, which exhibited auxin-cytokinin activity. The cost of these substitutes is much lower than that of commercial phytohormones. Conclusions. It is shown that optimal for growth in the MS medium in the callusogenesis of Solanacea cultures in vitro. That variant with the use of cytokinin substitutes Ecostim and Ecostim 1 with the rate of using of 35.0 and 40.0 mg/L. Keywords: modification of MS medium, potatoes, tomatoes, cell culture in vitro.

scholarly journals Peculiarities of in vitro parthenogenesis of unpollinated maize ovaries

2017 ◽  
Vol 6 (4) ◽  
pp. 10-13
Author(s):  
Tatyana Alekseevna Alatortseva
Keyword(s):  
Embryo Development ◽  
Nutrient Medium ◽  
Normal Development ◽  
Mother Plant ◽  
Maize Line ◽  
Mineral Components ◽  
Parthenogenetic Embryo ◽  
Direct Embryogenesis ◽  
Agricultural Plants

The maize line AT-1 is characterized by a hereditary predisposition to parthenogenesis. The aim of this investigation is to study parthenogenetic embryo development in the culture of unpollinated ovaries in vitro . The unpollinated ovaries were explanted in 1, 3, 5, 7, 10, 15 days after the appearance of stigmas from ears. The nutrient medium included mineral components of MS, vitamins, sucrose (9,0%), 2,4-D (2,0 mg/l), agar-agar. The structure of megagametophytes at the time of inoculation of the ovaries and on the 3rd, 7th, 14th, 21th, 28th day of cultivation was studied. The first divisions of unfertilized egg cells were observed on the 5th-7th day after appearance of stigmas from ears, independently from whether all this time the ovaries were on the mother plant or they were inoculated into the nutrient medium. The formation of the autonomous abnormal endosperm in some cultivated ovaries was detected. The abnormal endosperm disturbed normal development of the proembryo. As a rule, the ovaries with embryo and endosperm degenerated. In the absence of endosperm, the morphogenesis of parthenogenetic proembryos was carried out in one of two directions in vitro : 1) development of plants by direct embryogenesis; 2) regeneration of plants from numerous embryoids, raised on the surface of globular proembryos. The second direction was prevailed. The culture of unpollinated ovaries can be a promising method of mass haploid regenerants not only in maize, but also in other types of agricultural plants.

scholarly journals In vitro Cultivation of Potato Plants

2016 ◽  
Vol 5 (12) ◽  
pp. 858-868 ◽  
Author(s):  
Ibrahim A. Ibrahim ◽  
H.A. Emara ◽  
A.A. Nower ◽  
A.Y. Abodiab
Keyword(s):  
In Vitro Cultivation ◽  
Potato Plants

scholarly journals Clonal Micropropagation of Cranberry (Oxycoccus palustris Pers.)

2021 ◽  
Vol 51 (1) ◽  
pp. 67-76
Author(s):  
Sergey Makarov ◽  
Irina Kuznetsova ◽  
Mikhail Upadyshev ◽  
Sergey Rodin ◽  
Anton Chudetsky
Keyword(s):  
Survival Rate ◽  
Nutrient Medium ◽  
Average Length ◽  
Forest Products ◽  
Hybrid Form ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Total Growth

Introduction. The last decade saw a considerable increase in the demand for European cranberry planting material (Oxyccocus palustris Pers.) among consumers of non-timber forest products. Cranberry possesses high nutritional and medicinal value. Cultivars and hybrids of European cranberry prove extremely productive for plantation growth using the method of clonal micropropagation with revitalized planting material. Study objects and methods. The research featured European cranberry plants of the Dar Kostromy cultivar and its hybrid form 1-15-635. The study focused on the effect of various medications and growth regulators on the biometric profile of European cranberry and its adaptation to non-sterile conditions at all stages of in vivo clonal micropropagation. Results and discussion. During the introduction stage, the highest viability belonged to the explants treated with AgNO3 (95–96%) and Lizoformin 3000 (5%) as the main sterilizing solutions at a 10-min exposure and a 5% solution of Ecosterilizer (1:1) at a 20-min exposure (90–95%). During the micropropagation proper, the number, average length, and total growth of shoots increased as the concentration of cytokinin 2ip in the WPM 1/4 nutrient medium rose from 1.0 to 5.0 mg/L. At the stage of in vitro rooting, the maximal number, average length, and total growth of roots in regenerated plants for both cultivars were observed when Kornerost 5.0 mg/L was added to the WPM 1/4 nutrient medium. At the stage of adaptation to in vivo conditions, Micogel 0.2 mg/L contributed to the highest survival rate (94–100%). Conclusion. During clonal micropropagation in vitro, the biometric profile of European cranberry (Oxyccocus palustris Pers.) and its survival rate under non-sterile conditions in vivo proved to depend on various growth-regulating substances and their concentrations.

scholarly journals Regeneration capacity of Cerasus fruticosa and Prunus domestica into the in vitro culture

2021 ◽  
Vol 209 (06) ◽  
pp. 43-52
Author(s):  
Marina Markova ◽  
Elena Somova
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Optimal Time ◽  
In Vitro Cultivation ◽  
Initiation Time ◽  
Multiplication Factor ◽  
Optimal Conditions ◽  
Prunus Domestica ◽  
Nutrient Media

Abstract. The aim of these studies was to introduce into the in vitro culture the steppe cherry (Cerasus fruticosa) variety Shchedraya and the domestic plum (Prunus domestica) variety Sineokaya for subsequent micropropagation. Methods. Optimal conditions for obtaining viable explants, such as sterilizing agent and initiation time, have been investigated. The suitability of various nutrient media for in vitro cultivation of these cultures has also been tested. As a result of the experiments, it was revealed that the most effective sterilizing agents were 38 % perhydrol (control) and 6% chlorhexidine: the yield of viable cherry explants was 63.8 % and 61.5 %, plums – 69.8 % and 66.6 %, respectively. The optimal time for the initiation of cherry explants in vitro was January, where the yield of viable explants averaged 53.9 %, in June – 49.1 %, and for plums the initiation time did not matter – the yield of explants was 55.8 % in winter and 53.1 % in summer. In vitro cultivation of cherries and plums on the Quoirin – Lepoivre nutrient medium provided a significantly high multiplication factor, which averaged 4.1 for cherries (2.7 in control) and 6.0 for plums (3.9 in control). On the same medium, the maximum multiplication factor was obtained, which was 6.2 for cherries and 8.2 for plums. Thus, the scientific novelty of these studies is that the optimal conditions (sterilizing agent, time, nutrient medium) have been selected for the regeneration of cherry and plum explants in vitro with their subsequent micropropagation.

USING OF LARGE WAX MOTH LARVAE PRODUCTS EXCRETED IN CLONAL MICROREPRODUCTION OF GARDEN STRAWBERRY

1970 ◽  
pp. 66-68
Author(s):  
М. G. Markova ◽  
Е. N. Somova
Keyword(s):  
Aqueous Solution ◽  
Survival Rate ◽  
Nutrient Medium ◽  
Galleria Mellonella ◽  
Waste Product ◽  
Biologically Active ◽  
Clonal Micropropagation ◽  
Wax Moth

Work on the clonal micropropagation of strawberries comes down to the search for new growth regulators, which include a biologically active substance - the waste product of the wax moth Galleria mellonella L. The effect of the waste product of the wax moth on the efficiency of clonal micropropagation of strawberries (Fragaria х ananassa duch) in vitro and in vivo conditions in 2018-2020 is shown. The object of research is micro-cuttings, rooted micro-cuttings and adapted micro-plants of garden strawberries of the Korona variety and of the remontant strawberries of the Brighton variety. It was revealed that at the proliferation stage, the propagation coefficient of the Korona variety increased significantly with the introduction of the waste product of the wax moth in doses of 4.0 mg/L and 6.0 mg/L and amounted to 4.2 and 3.8 pcs./explant, respectively; for Brighton variety, the coefficient increased significantly when the dose of the waste product of the wax moth 2.0 mg/L and amounted to 4.6 pcs./explant. The introduction of the waste product of the wax moth in doses of 4.0 mg/L and 6.0 mg/L into the nutrient medium had a significant effect on the yield of Brighton micro-cuttings suitable for rooting: the yield was 95.5 and 94.1%, respectively 87.7% in the control. For the Korona variety, no significant positive effect of the waste product of the wax moth on this indicator was noted. The rooting of micro-cuttings of strawberries of both varieties significantly increased with the introduction of the waste product of the wax moth into the nutrient medium in all studied doses and amounted to 86.4-100% in the Korona variety, and 88.9-100% in the Brighton variety.  The survival rate of adaptable micro-cuttings of Corona variety strawberries when sprayed with an aqueous solution of the waste product of the wax moth at a dose of 4.0 mg/L was 100%; the maximum survival rate of micro-cuttings Brighton variety is 99.8% in the variant with spraying with an aqueous solution of the waste product of the wax moth at a dose of 6.0 mg/L.

SURVIVAL AND PRODUCTIVITY OF POTATO PLANTS OF LONG-TERM IN VITRO CULTIVATION IN THE CONDITIONS OF SOIL SUBSTRATE

2019 ◽  
Vol 2 (61) ◽  
pp. 28-33
Author(s):  
Saidvali F. Saydalizoda ◽  
◽  
Zarafo S. Kiyomova ◽  
Nigora N. Nazarova ◽  
Kurbon Aliev ◽  
...  
Keyword(s):  
In Vitro Cultivation ◽  
Soil Substrate ◽  
Potato Plants

scholarly journals Determination of xanthones in plants and the nutrient medium under in vitro cultivation conditions

2018 ◽  
Vol 76 (2) ◽  
pp. 33-37
Author(s):  
A. Revutska ◽  
V. Belava ◽  
A. Golubenko ◽  
N. Taran
Keyword(s):  
Nutrient Medium ◽  
Plant Material ◽  
In Vitro Cultivation ◽  
Cultivation Conditions ◽  
Quantitative Calculation ◽  
Environmentally Safe ◽  
Speed Up ◽  
Biotechnological Processes

In recent years, xanthones have received considerable attention from scientists due to their biological activity: anticarcinogenic, antiviral, antibacterial, antioxidant, anti-inflammatory and other properties.Therefore they are useful for prevention and treatment of different diseases:cancer, Alzheimer's and Parkinson's disease, cardiovascular disorders, diabetes, etc. Extracts of different species of plants containing xanthones are components of chemotherapeutic and other medical drugs. In order to find the most sensitive and environmentally safe method of quantitative determination of xanthones in the plant material and the nutrient medium, known methods were tested and selected for the prototype Vyisochina G. I. et al., 2011 method, which uses ethanol as an extractor. As the plant material we used plants of different species that were grown under in vitro cultivation conditions on the agarized nutrient medium. This agarized nutrient medium was also used for the xanthone content analysis. Based on the performed research, modifications of the method for determining the content of xanthones were adapted to the in vitro conditions, which detail the specificity of extraction and quantitative calculation of the xanthone content in plant explants. Our own method of determination of these compounds in the agarized nutrient medium was developed as well. The method, that we proposed, will significantly speed up the process of xanthone detecting and will also increase their yield in biotechnological processes for obtaining the pharmacologically valuable secondary metabolites of phenolic nature.

scholarly journals Significance of nutrient media choice for the long-term cultures of leukemic T-lymphoblasts

2021 ◽  
Vol 23 (3) ◽  
pp. 593-604
Author(s):  
L. S. Litvinova ◽  
K. A. Yurova ◽  
V. V. Shchupletsova ◽  
N. D. Gazatova ◽  
O. G. Khaziakhmatova ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Nutrient Medium ◽  
The Body ◽  
In Vitro Cultivation ◽  
Jurkat T Cells ◽  
Nutrient Media ◽  
Toxicological Studies

Correct choice of nutrient media for culturing different types of cells in various applications is one of the most important aspects of modern biotechnology, since chemical composition of the culture media largely contains the necessary metabolites to support certain cells’ growth lines outside the body. Jurkat line of human leukemic T-lymphoblast-like cells (hereinafter Jurkat T-cells) is actively used for in vitro modeling of intracellular signaling and activation of normal blood T-lymphocytes mediated by the T-cell receptor/CD3/ CD4 complex in toxicological studies of immune and secretory responses, to test medicinal substances and ions. Also, Jurkat T-cells are widely used for ex vivo testing in immunology, oncology, toxicology, orthopedics, and traumatology. The existing standards and numerous studies are mainly based on short-term in vitro cultivation of Jurkat T-cells in RPMI 1640 nutrient medium. Meanwhile, the issues of long-term maintenance of the growth of Jurkat T-cells culture are poorly presented in the research literature. This study aimed for studying the activity of Jurkat T-cells over 7 to 14 days of in vitro culture and comparing the relative value of RPMI 1640 and αMEM media for the behavior of immunocompetent tumor cells. Using flow cytometry, multiplex analysis, and phase contrast Cell-IQ microscopy, the proportions of living cells and those dying by apoptosis and necrosis, secretion of cytokines and chemokines, and the dynamics of cell biomass propagation were studied. It was found that the αMEM medium in the complete nutrient medium, as compared with RPMI 1640, is more appropriate to in vitro promotion of cell viability (increased proportion of viable cells by 13.5% at the day 14), their secretory ability for 23 из 27 tested biomolecules, shortened adaptation time (на 32%) in culture before growth initiation, 5-fold increase of the Jurkat Т-cell cellularity by the day 7. Potential significance of the chemical components of nutrient media and secreted biomolecules for these results is discussed. As based on the results obtained, we concluded on superior properties of αMEM medium for long-term in vitro cultures of Jurkat T-cells. Consequently, the in vitro testing of medical devices intended for long-term contact with the body, including those for cancer patients, using Jurkat T-cell leukemia line in RPMI 1640 medium, may lead to wrong predictions on their biocompatibility and potential antitumor activity.

scholarly journals Optimization of in vitro cultivation conditions for rare and endangered species of Berberis iliensis and Berberis karkaralensis

2020 ◽  
Vol 132 (3) ◽  
pp. 43-56
Author(s):  
V.K. Karimova ◽  
◽  
B.N. Baktybai ◽  
G.K. Magzumova ◽  
ZH. T. Sartbaev ◽  
...  
Keyword(s):  
Endangered Species ◽  
Nutrient Medium ◽  
Anthropogenic Activities ◽  
In Vitro Cultivation ◽  
Cultivation Conditions ◽  
Rare And Endangered Species ◽  
Optimization Study ◽  
Rare And Endangered ◽  
Living Organisms

Today, many living organisms are negatively affected by climate change and anthropogenic activities, which leads to a decrease in their numbers. One of these rare and endangered plant species is the Ili barberry (Berberis iliensis) and the Karkaraly barberry (Berberis karkaralensis). This work is devoted to the optimization study of the cultivation conditions for a rare and endangered species of Ili barberry and Karkaralinsky barberry in vitro. To obtain sterile and viable explants, the sterilizing agent was a solution of 0.5% «Domestos» (7 min). For the regeneration of the main shoot of the barberry, the optimal nutrient medium is Murashige and Skoogwith the addition of 6-benzylaminopurine- 0.5 mg/l, gibberellic acid- 1.0 mg/l, indole-3-butyric acid -0.01 mg/l, where regeneration was 80% for the Ili barberry, barberry karkaralinsky - 70%. For the multiplication of Berberis iliensis microshoots, the Quoirin & Lepoivre culture medium with the addition of 0.75 mg/l - benzylaminopurine is optimal; the number of microshoots formed was 3.6 per explant. Root formation is one of the most difficult stages in micropropagation. For the rooting of microshoots of Karkaralinsky barberry, a nutrient medium of ½ Murashige and Skoog was used with the addition of indolylbutyric acid -1.5 mg/l.

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