Improved Staining of Sex Chromatin: Ninety-Seven and Six Tenths Per Cent of Female Buccal Cells Chromatin-Positive

The previously reported low incidence of sex chromatin in buccal cells of newborn females has been confirmed in babies born by cesarean section. However, there was no comparable change in sex chromatin percentage for their mothers as had been reported for vaginally delivered women. Further studies on vaginally delivered mothers raised some doubt as to the validity of the previous maternal results.

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LOWER INCIDENCE OF SEX CHROMATIN IN BUCCAL SMEARS OF NEWBORN FEMALES

PEDIATRICS ◽

10.1542/peds.30.5.707 ◽

1962 ◽

Vol 30 (5) ◽

pp. 707-711

Author(s):

David W. Smith ◽

Philip M. Marden ◽

Michael J. McDonald ◽

Mark Speckhard

Keyword(s):

Newborn Infant ◽

Lower Incidence ◽

Postnatal Life ◽

Buccal Cells ◽

Buccal Smear ◽

Sex Chromatin ◽

Common Etiology

The incidence of the sex chromatin mass in buccal cells of newborn females is often lower during the first day or two of postnatal life, as compared to later ages. This finding allows for misinterpretation of the significance of buccal smear results during the first day or two. A similar phenomenon was noted in mothers during the day prior to delivery and for one to two days thereafter. This suggests a common etiology for this effect in both the mother and the newborn infant.

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Spontaneous Haemophilia in a Female

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654520 ◽

1960 ◽

Vol 04 (03) ◽

pp. 369-375 ◽

Cited By ~ 7

Author(s):

E. H Braun ◽

David B. Stollar

Keyword(s):

Family History ◽

Bleeding Time ◽

Dental Extraction ◽

White Girl ◽

Complete Family ◽

Normal Limits ◽

Sex Chromatin ◽

History Of ◽

Generation Test

SummaryA case of haemophilia in a young white girl is described. There was a history of bleeding from birth. The thromboplastin generation test was grossly abnormal and A. H. G. levels were below 1%. Bleeding time and capillary morphology was within normal limits. Dental extraction after transfusion caused almost uncontrollable haemorrhage.A complete family history was obtained for four generations. There was no case of a “bleeder” amongst these.The girl’s apparent sex was confirmed by sex chromatin studies.

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Differences in the H Antigen on Human Buccal Cells from Secretor and Non-Secretor Individuals

Vox Sanguinis ◽

10.1159/000465097 ◽

1966 ◽

Vol 11 (2) ◽

pp. 137-156

Author(s):

L.L. Flory

Keyword(s):

Buccal Cells

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Exposure to Particulate PAHs on Potential Genotoxicity and Cancer Risk among School Children Living Near the Petrochemical Industry

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18052575 ◽

2021 ◽

Vol 18 (5) ◽

pp. 2575

Author(s):

Nor Ashikin Sopian ◽

Juliana Jalaludin ◽

Suhaili Abu Bakar ◽

Titi Rahmawati Hamedon ◽

Mohd Talib Latif

Keyword(s):

Cancer Risk ◽

Environmental Protection Agency ◽

Primary Schools ◽

Petrochemical Industry ◽

Industrial Area ◽

Industrial Emissions ◽

Buccal Cells ◽

Genotoxic Effects ◽

Lifetime Cancer Risk ◽

Polycyclic Aromatic

This study aimed to assess the association of exposure to particle-bound (PM2.5) polycyclic aromatic hydrocarbons (PAHs) with potential genotoxicity and cancer risk among children living near the petrochemical industry and comparative populations in Malaysia. PM2.5 samples were collected using a low-volume sampler for 24 h at three primary schools located within 5 km of the industrial area and three comparative schools more than 20 km away from any industrial activity. A gas chromatography–mass spectrometer was used to determine the analysis of 16 United States Environmental Protection Agency (USEPA) priority PAHs. A total of 205 children were randomly selected to assess the DNA damage in buccal cells, employing the comet assay. Total PAHs measured in exposed and comparative schools varied, respectively, from 61.60 to 64.64 ng m−3 and from 5.93 to 35.06 ng m−3. The PAH emission in exposed schools was contributed mainly by traffic and industrial emissions, dependent on the source apportionment. The 95th percentiles of the incremental lifetime cancer risk estimated using Monte Carlo simulation revealed that the inhalation risk for the exposed children and comparative populations was 2.22 × 10−6 and 2.95 × 10−7, respectively. The degree of DNA injury was substantially more severe among the exposed children relative to the comparative community. This study reveals that higher exposure to PAHs increases the risk of genotoxic effects and cancer among children.

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Y Chromosomes in Male Psychiatric Patients above 180 cm. Tall

The British Journal of Psychiatry ◽

10.1192/bjp.114.517.1589 ◽

1968 ◽

Vol 114 (517) ◽

pp. 1589-1590 ◽

Cited By ~ 15

Author(s):

Johannes Nielsen

Keyword(s):

Chromosome Number ◽

State Hospital ◽

Psychiatric Patients ◽

Chromosome Analysis ◽

Y Chromosomes ◽

Sex Chromatin ◽

Male Patients

All male patients above 180 cm. tall who were resident in the Århus State Hospital on 13 April, 1966, were registered: they numbered 42 out of the total of 440 resident males (8 · 1 per cent.). One patient, a 78-year-old man, died before chromosome analysis was made. Sex-chromatin analysis was made on Feulgenstained buccal smears and chromosome analysis was made on leucocyte cultures according to the method described by Moorhead et al. (1960), slightly modified. Not less than 25 metaphases were counted, at least 15 metaphases with the modal figure and all metaphases with a chromosome number deviating from the modal figures were analysed.

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Impact of Preterm Birth on the Cellular Characteristics of Neonatal Buccal Cells

Cytopathology ◽

10.1111/cyt.12992 ◽

2021 ◽

Author(s):

Hanife Guler Donmez ◽

Hasan Tolga Celik ◽

Gozdem Kayki ◽

Sule Yigit ◽

Murat Yurdakok ◽

...

Keyword(s):

Preterm Birth ◽

Buccal Cells

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Inhibition of microbial adherence by sphinganine

Canadian Journal of Microbiology ◽

10.1139/m92-158 ◽

1992 ◽

Vol 38 (9) ◽

pp. 983-985 ◽

Cited By ~ 22

Author(s):

Debra Jan Bibel ◽

Raza Aly ◽

Henry R. Shinefield

Keyword(s):

Staphylococcus Aureus ◽

Infectious Diseases ◽

Therapeutic Agents ◽

Eukaryotic Cells ◽

Buccal Cells ◽

Streptococcus Mitis ◽

Buccal Epithelial Cells ◽

Mucosal Cells ◽

Microbial Adherence ◽

Complex Sphingolipids

Sphingosines (precursors and degeneration products of complex sphingolipids) are mediators in membrane second-messenger cascades and in a wide variety of functions in eukaryotic cells. Sphingosines are also lethal for gram-positive microorganisms. In addition to its direct effect, sphinganine is here reported to affect the adherence of Streptococcus mitis to buccal epithelial cells and of Staphylococcus aureus to nasal mucosal cells after incubation for 90 min at 37 °C. When the bacteria were pretreated with 8.1, 16.2, 32.5, or (for Strep. mitis) 65 μM sphinganine for 60 min at 37 °C, adherence counts were reduced for Staph. aureus by 27, 37, and 60% and for Strep. mitis by 19, 44, 54, and 73%, respectively (p < 0.001). In contrast, pretreatment of buccal cells with 81.2 μM lipid increased adherence by 14% (p < 0.01), but no change occurred at either 16.2 or 325 μM lipid. These results further demonstrate the double-edged ability of sphingosines to regulate cellular activities and their potential as multifunctional therapeutic agents for infectious diseases. Key words: adherence, sphingosine, Staphylococcus aureus, Streptococcus mitis.

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