The Effects of a Novel Magnesium Complex on the Intestinal Magnesium Transporters in HFD Rats
Abstract Objectives Magnesium (Mg) is absorbed mainly in the intestine and regulates many physiological processes and signaling pathways. TRPM6, a member of the transient receptor potential melastatin-related (TRPM) protein family, is essential for maintaining the organismal Mg2 + balance in the intestine. This study was done to compare the effects of a new form of magnesium, Mg-N21, to magnesium oxide (MgO) on the expression of intestinal Mg transporters in rats Methods Forty-two male Wistar rats (age: 8-week) were randomly divided into the following groups (n = 7 per group): 1) Control; 2) MgO; 3) Mg-N21; 4) HFD (high fat diet); 5) HFD + MgO; 6) HFD + Mg-N21. Magnesium was dosed at 500 milligrams of elemental Mg/kg diet. All rats were supplemented for 8 weeks. Results HFD intake showed a trend toward decreased ileum TRPM6 expression in rats. However, Mg treatment increased ileum TRPM6 expression in HFD rats. TRPM7 expression was not significantly changed. In addition, claudins (Cldn-12 and −15) expression decreased in the ileum of rats fed a HFD. The expressions of ileum claudins were enhanced in Mg-treated rats. The effectiveness of Mg as Mg-21 was more pronounced than Mg as MgO, which can be associated with higher bioavailability. Conclusions Results of the present study show that Mg-N21 is more protective than magnesium oxide on the Mg transporters in rats fed HFD. Funding Sources JDS Therapeutics, LLC Turkish Academy of Sciences.