An Automated Method for the Determination of Estrogens in Pregnancy

1968 ◽  
Vol 14 (10) ◽  
pp. 1010-1022 ◽  
Author(s):  
D Ua Conaill ◽  
G G Muir
Keyword(s):  
Turnover Rate ◽  
Good Recovery ◽  
Manual Method ◽  
Color Development ◽  
Automated Method ◽  
Combined Operation ◽  
In Pregnancy

Abstract Ittrich’s method for the determination of total estrogens in urine (1) was adapted for automation on the AutoAnalyzer, using a continuous digester module for the combined operation of phase exchange and color development. The automated method gave good recovery and reproducibility, and correlated well with the manual method. It increased the capacity of this laboratory for estrogen determinations, improved the turnover rate, and reduced the labor involved to a minimum.

An automated method for the determination of estrogens in pregnancy urine

1973 ◽  
Vol 6 ◽  
pp. 34-40 ◽  
Author(s):  
A. Craig ◽  
J.W. Leek ◽  
R.F. Palmer
Keyword(s):  
Automated Method ◽  
Pregnancy Urine ◽  
In Pregnancy

Automated Colorimetric Determination of Phenylephrine Hydrochloride in Drug Formulations. II. Collaborative Study

1971 ◽  
Vol 54 (3) ◽  
pp. 600-602
Author(s):  
Michel Margosis
Keyword(s):  
Coefficient Of Variation ◽  
Statistical Evaluation ◽  
Collaborative Study ◽  
Colorimetric Method ◽  
Colorimetric Determination ◽  
Good Recovery ◽  
Drug Formulations ◽  
Phenylephrine Hydrochloride ◽  
Automated Method

Abstract The automated colorimetric method designed by Lane for the analysis of phenylephrine HCl in drug formulations has been submitted to a collaborative study. Five samples, including 2 with known interferences, were sent to 9 collaborators. The results were subjected to statistical evaluation. These show good recovery and a maximum interlaboratory coefficient of variation of 4.5%. The automated method as evaluated in this study has been adopted as official first action.

Automated Simultaneous Determination of p-Aminohippurate and Creatinine in Plasma or Urine

1974 ◽  
Vol 20 (3) ◽  
pp. 348-352 ◽  
Author(s):  
C K Parekh ◽  
J Kirpan ◽  
A Peterson ◽  
G L Hassert ◽  
B F Murphy
Keyword(s):  
Simultaneous Determination ◽  
Urine Samples ◽  
Manual Method ◽  
Automated Method ◽  
Discrete Sample ◽  
Beer's Law ◽  
Plasma And Urine Samples ◽  
Beer’S Law

Abstract Methods have been developed for using the Beckman "Discrete Sample Analyzer" (DSA-560), for automated simultaneous determinations of p-aminohippurate (PAH) and creatinine in the same 50- or 10-µl samples of plasma or urine, respectively, at the rate of 80 samples per hour. In determinations of PAH, a single reagent, p-dimethylaminocinnamaldehyde in 0.1 mol/liter HCl, was added to a protein-free filtrate from plasma or urine. The intensity of the color, measured at 550 nm, obeyed Beer’s law for PAH concentrations from 0.005 to 5.0 g/liter. Data obtained for the same plasma and urine samples by the manual method, were, in general, within ±5% of the results obtained by the automated method. Creatinine was simultaneously determined by adapting the method of Taussky to the DSA-560 instrument; the intensity of the color, measured at 510 nm, obeyed Beer's law for creatinine concentrations ranging from 0.005 to 5.0 g/liter. Data obtained for the same plasma and urine samples by the manual method, were, in general, within ± 5% of the results obtained by the automated method.

scholarly journals An Automated Method for the Determination of Intestinal Disaccharidase and Glucoamylase Activities

2006 ◽  
Vol 2006 ◽  
pp. 1-4 ◽  
Author(s):  
Zhaoping He ◽  
Laura Bolling ◽  
Dalal Tonb ◽  
Tracey Nadal ◽  
Devendra I. Mehta
Keyword(s):  
Quality Control ◽  
Automated System ◽  
Internal Quality ◽  
Perfect Agreement ◽  
Manual Method ◽  
Automated Method ◽  
Reaction Volumes ◽  
High Consistency ◽  
Larger Sample

Determination of disaccharidase and glucoamylase activities is important for the diagnosis of intestinal diseases. We adapted a widely accepted manual method to an automated system that uses the same reagents reaction volumes, incubation times, and biopsy size as the manual method. A dye was added to the homogenates as the internal quality control to monitor the pipetting precision of the automated system. When the automated system was tested using human intestinal homogenates, the activities of all the routinely tested disaccharidases, including lactase, maltase, sucrase, and palatinase, as well as the activity of glucoamylase, showed perfect agreement with the manual method and were highly reproducible. The automated analyzer can perform the same routine assays of disaccharidases and glucoamylase with high consistency and accuracy and reduce testing costs by performing a larger sample size with the same number of staff. Additional developments, such as barcoding and built-in plate reading, would result in a completely automated system.

Automated Determination of Serum Ceruloplasmin Activity with o-Dianisidine Dihydrochloride as Substrate

1975 ◽  
Vol 21 (6) ◽  
pp. 757-759 ◽  
Author(s):  
Karl H Schosinsky ◽  
Peter Lehmann ◽  
Myrton F Beeler
Keyword(s):  
Coefficient Of Variation ◽  
Serum Ceruloplasmin ◽  
Manual Method ◽  
Automated Method ◽  
Enzymatic Determination ◽  
Ceruloplasmin Activity ◽  
Automated Determination

Abstract An automated method for the enzymatic determination of ceruloplasmin with o-dianisidine dihydrochioride as substrate is described. The method enables the measurement of 30 samples per hour with a coefficient of variation (day-to-day) of 2.8%. Results correlate well (r = 0.99) with those obtained by the corresponding manual method.

Determination of Serum or Plasma Glucose on the "AutoAnalyzer II" by Use of the Hexokinase Reaction

1972 ◽  
Vol 18 (3) ◽  
pp. 299-300 ◽  
Author(s):  
G M Widdowson ◽  
J R Penton
Keyword(s):  
Plasma Glucose ◽  
Good Precision ◽  
Manual Method ◽  
Automated Method

Abstract An automated method is described for determination of glucose in serum or plasma by use of the hexokinase reaction. The method has good precision and the results correlate well with those from a manual method in which the same reaction is used.

An Automated Method for Measuring Creatine Phosphokinase Activity in Serum

1970 ◽  
Vol 16 (4) ◽  
pp. 294-299 ◽  
Author(s):  
Ronald K Wright ◽  
Roy L Alexander
Keyword(s):  
Enzyme Activity ◽  
Enzyme Activities ◽  
Creatine Phosphokinase ◽  
Creatine Phosphate ◽  
Enzyme Concentration ◽  
Magnesium Ion ◽  
Manual Method ◽  
Automated Method ◽  
Automated Procedures

Abstract We describe a procedure for automating the determination of creatine phosphokinase (CPK) activity in serum by use of the AutoAnalyzer. Enzyme activity is determined by measuring the creatine phosphate formed from the CPK-catalyzed reaction of creatine with adenosine triphosphate (ATP). The sensitivity of the automated procedure was comparable to that of the manual method. By use of the most favorable concentrations of creatine, ATP, and magnesium ion in the substrate, a linear relationship was obtained between enzyme concentration and enzyme activities up to 600 mU, representing a sixfold improvement over that obtained by the manual method. The degree of correlation between results obtained by the manual and automated procedures is shown.

Automated Determination of Thiamine (Vitamin B1) in Pharmaceutical Preparations

1971 ◽  
Vol 54 (5) ◽  
pp. 1164-1167
Author(s):  
Omer Pelletier ◽  
RenÉ Brassard
Keyword(s):  
Liver Extract ◽  
Vitamin B1 ◽  
Pharmaceutical Preparations ◽  
Manual Method ◽  
Automated Method ◽  
Thiamine Content ◽  
Aoac Method ◽  
Automated Determination

Abstract The chemical principles used for the determination of thiamine in pharmaceutical preparations by the official USP and AOAC methods were adapted to an automated flowthrough analyzer. The system could analyze 20 samples (including blanks) per hour. Thiamine extracts from 12 different multivitamin preparations were analyzed by one analyst on 5 different days by the automated method and the AOAC method. Both methods yielded comparable values for 10 of the preparations. The thiamine content of 2 preparations containing liver extract was found to be about 9% higher by the automated method than by the AOAC method. Further analysis of other samples of these 2 preparations containing liver extract showed that chromatography on Decalso did not change the results obtained by the automated method, but raised the results obtained by the manual method to a level comparable to the automated method. The AOAC method showed a tendency for a slightly higher reproducibility of assays.

Automated Method for Determination of Serum Calcium by Use of Alizarin

1970 ◽  
Vol 16 (10) ◽  
pp. 816-819 ◽  
Author(s):  
Christopher S Frings ◽  
Patricia S Cohen ◽  
Lowell B Foster
Keyword(s):  
Atomic Absorption ◽  
Serum Calcium ◽  
Coefficient Of Variation ◽  
Atomic Absorption Spectrophotometry ◽  
Manual Method ◽  
Absorption Spectrophotometry ◽  
Automated Method ◽  
Beer's Law ◽  
Beer’S Law

Abstract An automated method is described for determining serum calcium by measuring the red chromogenic complex formed by calcium and alizarin. About 40 µl of undialyzed serum is added directly to one reagent to pro-duce the color, which follows Beer's law at 599 nm to a concentration of 15.0 mg of calcium per 100 ml, with a coefficient of variation of 2.5%. Serum calcium concentrations, as measured by our method, compare favorably with those by the corresponding manual method and by atomic absorption spectrophotometry

Turbidimetric determination of prothrombin time by clotting in a centrifugal analyzer.

1986 ◽  
Vol 32 (10) ◽  
pp. 1857-1862 ◽  
Author(s):  
G O Gogstad ◽  
K H Dahl ◽  
A Christophersen ◽  
A Bjerke
Keyword(s):  
Prothrombin Time ◽  
Small Deviation ◽  
Clot Formation ◽  
Plasma Samples ◽  
Manual Method ◽  
Automated Method ◽  
Reagent Cost ◽  
Turbidimetric Measurement ◽  
Total Absorbance

Abstract Two thromboplastin reagents ("Thrombotest" and "Normotest Automated") were used in evaluation of an automated method for determination of prothrombin time based on turbidimetric measurement of clot formation in a centrifugal analyzer. We used 60 plasma samples from patients with various diseases or being treated with oral anticoagulant and 16 normal plasma samples. Prothrombin times were calculated by a computer connected to the analyzer, a reading being made at either a certain per cent increase in total absorbance or a fixed absorbance increase. Both correlated well with the manual method (r = 0.98-0.99). The reading points best fitting the manually obtained data were estimated by minimizing the residual sum of squares in regression analyses performed at various absorbance increases. The per cent reading was better in this respect. Normotest Automated could be nearly perfectly related to the manual method, whereas Thrombotest showed a (negligibly) small deviation. Reproducibility was good within run (CV less than or equal to 3.2%) as well as between batch of the reagents, as assessed from variation in INR (CV less than or equal to 4.9%). We conclude that turbidimetry of clot formation may be validly used in automation of the prothrombin-time test. The equipment needed and the total time per analysis are about as for chromogenic substrate methods, but reagent cost is considerably lower.

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