Effect of transport conditions on the stability of biochemical markers in blood.

1989 ◽  
Vol 35 (12) ◽  
pp. 2313-2316 ◽  
Author(s):  
S E Hankinson ◽  
S J London ◽  
C G Chute ◽  
R L Barbieri ◽  
L Jones ◽  
...  
Keyword(s):  
Whole Blood ◽  
Apolipoprotein B ◽  
Biochemical Markers ◽  
Room Temperature ◽  
Density Lipoprotein ◽  
Alpha Tocopherol ◽  
Endogenous Hormones ◽  
Blood Samples ◽  
Whole Blood Samples ◽  
The Stability

Abstract We examined the stability of lipids, carotenoids, alpha-tocopherol, and endogenous hormones in plasma prepared from whole blood that had been mailed to a central location for processing. Initially, to simulate transport conditions, whole-blood samples were stored in the laboratory, either at room temperature or cooled, for up to 72 h before processing. In the latter samples, lipid concentrations changed up to 1.4% per day, carotenoids up to -5.5%, and hormones up to 9.5%. In a second study, analyte concentrations in plasma from cooled whole blood mailed via overnight courier were compared with those from plasma that had been immediately separated, frozen, and mailed via overnight courier. Concentrations of cholesterol, high-density lipoprotein subfraction 3, apolipoprotein B, and retinol were stable. Overall, for each marker except estradiol, the between-person variation was at least twice the within-person variation. In a third study, at least 340 micrograms of DNA was recovered from 30 mL of cool-shipped whole blood. Our results indicate that shipping whole-blood samples by overnight courier is feasible for assay of several biochemical markers of interest in epidemiological research.

scholarly journals Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
İbrahim Kaplan ◽  
Hatice Yüksel ◽  
Osman Evliyaoğlu ◽  
M. Kemal Basarali ◽  
Gülten Toprak ◽  
...  
Keyword(s):  
Cyclosporine A ◽  
Whole Blood ◽  
Room Temperature ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Blood Samples ◽  
Percent Change ◽  
Significant Difference ◽  
Whole Blood Samples ◽  
The Stability

Tacrolimus and cyclosporine A are immunosuppressant drugs with narrow therapeutic windows. The aim of this study was to investigate the stability of tacrolimus and cyclosporin A levels in whole blood samples under different storage conditions. Whole blood samples were obtained from 15 patients receiving tacrolimus and 15 patients receiving cyclosporine A. Samples were immediately analyzed and then stored at different conditions (room temperature (24°C−26°C) for 24 hours, +4°C for 24 and 48 hours, and −20°C for one month) and then analyzed again. For tacrolimus, there was a significant difference between samples analyzed immediately and those kept 24 hours at room temperature (P=0.005) (percent change 32.89%). However, there were no significant differences between the other groups. For cyclosporine A, there was a significant difference between samples analyzed immediately and those kept 24 hours (P=0.003) (percent change 19.47%) and 48 hours (P=0.002) (percent change 15.38%) at +4°C and those kept 24 hours at room temperature (P=0.011) (percent change 9.71%). Samples of tacrolimus should be analyzed immediately or stored at either +4°C or −20°C, while samples of cyclosporine A should be analyzed immediately or stored at −20°C.

Whole blood samples for adrenocorticotrophic hormone measurement can be stored at room temperature for 4 hours

2016 ◽  
Vol 76 (8) ◽  
pp. 653-656 ◽  
Author(s):  
Mette Christensen ◽  
Rikke Fogt Madsen ◽  
Line Rosengreen Møller ◽  
Cindy Soendersoe Knudsen ◽  
Mie Hessellund Samson
Keyword(s):  
Whole Blood ◽  
Room Temperature ◽  
Adrenocorticotrophic Hormone ◽  
Blood Samples ◽  
Whole Blood Samples

Long-Term Preservation of Whole Blood Samples for Flow Cytometry Analysis in Normal and HIV-Infected Individuals from Africa

1990 ◽  
Vol 1 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Renu B Lal ◽  
Subhash K Hira ◽  
Rita R Dhawan ◽  
Peter L Perine
Keyword(s):  
Flow Cytometry ◽  
Whole Blood ◽  
Phenotypic Analysis ◽  
Blood Samples ◽  
Remote Areas ◽  
Immunodeficiency Virus ◽  
Whole Blood Samples ◽  
The Stability ◽  
Proliferation Assays ◽  
Lymphocyte Phenotypes

A whole blood method requiring less than 4 ml of heparinized blood was developed to assess the practicality of preparing whole blood samples that could be easily stored, transported and readily used to determine the lymphocyte phenotypes and proliferation responses of individuals from remote areas who are infected with the human immunodeficiency virus. Minor modifications in standard whole blood procedure for lymphocyte phenotyping have significantly increased the stability of light scatter and fluorescence intensity of the cells for subsequent flow cytometry (FC) analysis. These changes include removal of lysis solution prior to fixation, fixation of monoclonal antibody-stained cells in 1% paraformaldehyde for 30 minutes and storage of fixed samples in medium containing 1% bovine serum albumin. Lymphocyte subsets and their functional subsets could reliably be determined on samples stored for up to 4 weeks. Further, blood samples could be kept at room temperature for up to 96 hours or at ambient temperature during transportation from Africa before staining for FC without affecting their quantitation. While samples could be processed for FC analysis under field-laboratory conditions, proliferation assays could only be performed on samples that were transported within 48 hours of their collection. The whole blood method saves time and expense and decreases the volumes of blood required to perform phenotypic analysis and functional assays on specimens collected in remote areas.

Effects of storage temperature and time before centrifugation on ionized calcium in blood collected in plain vacutainer tubes and silicone-separator (SST) tubes.

1984 ◽  
Vol 30 (4) ◽  
pp. 553-556 ◽  
Author(s):  
J Toffaletti ◽  
N Blosser ◽  
K Kirvan
Keyword(s):  
Room Temperature ◽  
Storage Temperature ◽  
Ionized Calcium ◽  
Blood Collection ◽  
Healthy Individuals ◽  
Blood Samples ◽  
The Mean ◽  
Whole Blood Samples ◽  
The Stability ◽  
Blood Collection Tubes

Abstract We studied the stability of ionized calcium and pH in samples stored at either room temperature or 4 degrees C, in centrifuged and uncentrifuged blood-collection tubes and in centrifuged tubes containing a silicone-separator gel (SST tubes). At room temperature, in uncentrifuged blood from healthy individuals, mean ionized calcium usually increased no more than 10 mumol/L per hour; at 4 degrees C it did not change detectably for 70 h. This stability was fortuitous, however: the concentrations of both hydrogen and lactate ions in these samples increased, apparently with offsetting effects on the concentration of ionized calcium. Blood stored for 70 h at 4 degrees C in centrifuged SST tubes, although showing a slightly greater change in ionized calcium, had less change of pH and no change in the ionized calcium corrected to pH 7.4. In 11 heparinized whole-blood samples from eight patients in intensive care, the mean change per hour in ionized calcium and pH after storage at room temperature was +10 mumol/L and -0.04 units, respectively.

scholarly journals Stability of Routine Hematology Sample Using The Medonic M-Series Analyzer

2020 ◽  
Vol 6 (2) ◽  
pp. 108
Author(s):  
Eva Ayu Maharani ◽  
Dewi Astuti
Keyword(s):  
Room Temperature ◽  
Storage Conditions ◽  
Healthy Individuals ◽  
Fresh Sample ◽  
Blood Samples ◽  
Significant Difference ◽  
Hematology Analyzer ◽  
Whole Blood Samples ◽  
The Stability ◽  
Wbc Count

Routine hematology tests (Hb, Hct, RBC, WBC, PLT) generally done by automation methods using a hematology analyzer. Ideally, the examinations should be done as soon as possible, although, in some circumstances, it can be delayed. Based on the literature, the sample for routine hematology testing should be processed within 4 – 8 hours of venesection. Some studies revealed that the sample could be stored for up to 48 hours, and it can be influenced by the technology applied to the hematology analyzer. Studies conducted to see the stability of the samples using a hematology analyzer with impedance technology. Tests performed on whole blood samples collected from 12 ostensibly healthy individuals, immediately after collection (fresh sample, <1hour) and 2h, 4h, 6h, 8h, 24h, 48h afterward. The samples stored at room temperature (20-240C) and 2-60C. There are no significant differences after 48 h under different storage conditions for Hb, Hct, RBC, PLT count, except for WBC count that has a significant difference at temperature 2-60C. CV for Hb, Hct, RBC, PLT, WBC count is less than 5% at room temperature. WBC and PLT count have a CV of more than 5% at 2-60 C. Sample for Hb, Hct, RBC was found to be stable up to 48 h at room temperature and 2-60C. PLT and WBC count were stable for 48 h if stored at room temperature.

Pre-analytical variation in alanine aminotransferase.

1988 ◽  
Vol 34 (4) ◽  
pp. 744-745 ◽  
Author(s):  
S G Ruby ◽  
N E Reiber ◽  
R E Lonser
Keyword(s):  
Analysis Of Variance ◽  
Whole Blood ◽  
Alanine Aminotransferase ◽  
Room Temperature ◽  
Normal Activity ◽  
Blood Samples ◽  
Activity Concentrations ◽  
Whole Blood Samples ◽  
Blood Specimens ◽  
Analytical Variation

Abstract To determine the effect of pre-analytical variation of alanine aminotransferase in blood specimens with normal activity concentrations of this enzyme, we stored serum and whole blood samples at 4 and 22 degrees C and assayed aliquots of each specimen at intervals up to 72 h. Analysis of variance revealed no statistically significant increase or decrease in activity of the enzyme for up to 72 h in either specimen type, whether stored at room temperature or refrigerated.

Ascorbic Acid Improves the Stability of Buprenorphine in Frozen Whole Blood Samples

2019 ◽  
Vol 43 (6) ◽  
pp. 482-488 ◽  
Author(s):  
Lambert K Sørensen ◽  
Jørgen B Hasselstrøm
Keyword(s):  
Ascorbic Acid ◽  
Whole Blood ◽  
Storage Period ◽  
Blood Samples ◽  
The Mean ◽  
Whole Blood Samples ◽  
The Stability

Abstract Buprenorphine (BUP) was not long-term stable in whole blood samples preserved with fluoride citrate (FC) and fluoride oxalate (FX) mixtures when stored at −20°C. On average, only half of the initial concentrations of BUP was recovered after 12 weeks of storage at −20°C when interrupted by 3–4 thaw/freeze cycles. Norbuprenorphine (NBUP) was less unstable; approximately 90% was recovered after 12 weeks of storage at −20°C. The instability was less at 5°C, but the formation of BUP and NBUP from their glucuronides was observed at that temperature, especially in FC-preserved blood. The substances were stable for at least 5 months when stored uninterrupted at −80°C. The instability of BUP and NBUP in FC- and FX-preserved whole blood stored at −20°C was eliminated when the samples were modified with 30 mM ascorbic acid (ASC) prior to storage. The mean recoveries were greater than 95% after a 5-month interrupted storage period at −20°C when modified with ASC.

scholarly journals Extended storage of whole blood with 3-deazaadenosine for homocysteine assay

2007 ◽  
Vol 44 (4) ◽  
pp. 388-390
Author(s):  
Monica Hansrani ◽  
Gerard Stansby
Keyword(s):  
Whole Blood ◽  
Room Temperature ◽  
Statistical Difference ◽  
Plasma Homocysteine ◽  
Blood Samples ◽  
Time Points ◽  
Whole Blood Samples ◽  
Significant Change

Background: To assess the effectiveness of using 3-deazaadenosine (3DAA) to maintain plasma homocysteine concentrations (tHCy) in whole blood samples. Methods: Blood was obtained from five volunteers and samples were maintained at room temperature, in cold packs or in a fridge (0-4°C) with and without 3DAA. At time points ranging from 6 to 168 h, samples were processed and analysed for tHCy using the Abbott IMx system. Results: There was a mean increase in tHCy of 29.4% at 6 h increasing to 242.6% after 168 h in whole blood kept at room temperature. There was no significant change in tHCy for 48 h when stored in cold packs, and for 72 h when stored in the fridge. The addition of 3DAA had a significant preservative effect ( P<0.001), maintaining tHCy to 48 h in whole blood at room temperature, 120 h in the fridge and 96 h in cool packs. There was no statistical difference in results obtained from samples containing preservative and controls when using the Abbott IMx system. Conclusion: 3DAA is an effective preservative of tHCy in whole blood, particularly in samples maintained at 0-4°C.

scholarly journals Stability of Routine Hematology Sample Using The Medonic M-Series Analyzer

2020 ◽  
Vol 6 (2) ◽  
pp. 108
Author(s):  
Eva Ayu Maharani ◽  
Dewi Astuti
Keyword(s):  
Room Temperature ◽  
Storage Conditions ◽  
Healthy Individuals ◽  
Fresh Sample ◽  
Blood Samples ◽  
Significant Difference ◽  
Hematology Analyzer ◽  
Whole Blood Samples ◽  
The Stability ◽  
Wbc Count

Routine hematology tests (Hb, Hct, RBC, WBC, PLT) generally done by automation methods using a hematology analyzer. Ideally, the examinations should be done as soon as possible, although, in some circumstances, it can be delayed. Based on the literature, the sample for routine hematology testing should be processed within 4 – 8 hours of venesection. Some studies revealed that the sample could be stored for up to 48 hours, and it can be influenced by the technology applied to the hematology analyzer. Studies conducted to see the stability of the samples using a hematology analyzer with impedance technology. Tests performed on whole blood samples collected from 12 ostensibly healthy individuals, immediately after collection (fresh sample, <1hour) and 2h, 4h, 6h, 8h, 24h, 48h afterward. The samples stored at room temperature (20-240C) and 2-60C. There are no significant differences after 48 h under different storage conditions for Hb, Hct, RBC, PLT count, except for WBC count that has a significant difference at temperature 2-60C. CV for Hb, Hct, RBC, PLT, WBC count is less than 5% at room temperature. WBC and PLT count have a CV of more than 5% at 2-60 C. Sample for Hb, Hct, RBC was found to be stable up to 48 h at room temperature and 2-60C. PLT and WBC count were stable for 48 h if stored at room temperature.

Sign in / Sign up

Export Citation Format

Share Document