Deciphering Operation of Tryptophan Independent Pathway in High Indole -3-Acetic Acid (IAA) producing Micrococcus aloeverae DCB-20

2020 ◽  
Author(s):  
Ees Ahmad ◽  
Sushil K Sharma ◽  
Pawan K Sharma
Keyword(s):  
Acetic Acid ◽  
Hplc Analysis ◽  
Simple Technique ◽  
Chromobacterium Violaceum ◽  
First Report ◽  
Acid Hydrolysate ◽  
Iaa Production ◽  
Reasonable Evidence ◽  
Indole 3 Acetic Acid ◽  
Iaa Biosynthesis

Abstract Genus Micrococcus is considered a high IAA producer. However, interestingly, there is no report on the tryptophan independent pathway operation in this genus. Consequently, the present study was undertaken to evaluate high IAA production by Micrococcus aloeverae DCB-20 and generate reasonable evidence for the occurrence of the tryptophan-independent pathway. Strain DCB-20 produced a high quantity of 880.51 µM or 154.3 µg ml−1 IAA in LB broth supplemented with L-tryptophan. The tryptophan independent pathway operation was supported by IAA production in Tris-minimal broth (TM broth) medium supplemented with acid hydrolyzed casein, which lacks tryptophan. The HPLC analysis showed the absence of tryptophan either from exogenous or endogenous sources in TM broth in the presence of casein acid hydrolysate inoculated with M. aloeverae DCB-20. The absence of tryptophan was further confirmed by the appearance of non-pigmented colonies of Chromobacterium violaceum TRFM-24 on Tris-minimal agar (TM agar) containing acid-hydrolyzed casein. This is probably the first report on IAA biosynthesis by M. aloeverae DCB-20 employing tryptophan independent pathway. This simple technique can also be adapted to detect operation of the tryptophan independent pathway in other bacteria.

scholarly journals In Planta Production of Indole-3-Acetic Acid by Colletotrichum gloeosporioides f. sp. aeschynomene

2004 ◽  
Vol 70 (3) ◽  
pp. 1852-1854 ◽  
Author(s):  
Rudy Maor ◽  
Sefi Haskin ◽  
Hagit Levi-Kedmi ◽  
Amir Sharon
Keyword(s):  
Acetic Acid ◽  
Fungal Biomass ◽  
Colletotrichum Gloeosporioides ◽  
Pathogenic Fungus ◽  
Plant Colonization ◽  
In Planta ◽  
Iaa Production ◽  
Axenic Cultures ◽  
Indole 3 Acetic Acid ◽  
Iaa Biosynthesis

ABSTRACT The plant pathogenic fungus Colletotrichum gloeosporioides f. sp. aeschynomene utilizes external tryptophan to produce indole-3-acetic acid (IAA) through the intermediate indole-3-acetamide (IAM). We studied the effects of tryptophan, IAA, and IAM on IAA biosynthesis in fungal axenic cultures and on in planta IAA production by the fungus. IAA biosynthesis was strictly dependent on external tryptophan and was enhanced by tryptophan and IAM. The fungus produced IAM and IAA in planta during the biotrophic and necrotrophic phases of infection. The amounts of IAA produced per fungal biomass were highest during the biotrophic phase. IAA production by this plant pathogen might be important during early stages of plant colonization.

scholarly journals Virulence and indole-3-acetic acid (IAA) biosynthesis ability of Turkish Pseudomonas savastanoi pv. savastanoi isolates and susceptibility of some native olive genotypes

2021 ◽  
Vol 19 (4) ◽  
pp. e1003-e1003
Author(s):  
Nursen Ustun ◽  
Keyword(s):  
Acetic Acid ◽  
Geographical Variation ◽  
Geographical Origin ◽  
Iaa Production ◽  
Complete Resistance ◽  
Mediterranean Regions ◽  
Pathogenicity Tests ◽  
Indole 3 Acetic Acid ◽  
Geographical Locations ◽  
Iaa Biosynthesis

Aim of study: To evaluate the virulence and indole-3-acetic acid (IAA) biosynthesis ability of several Turkish P. savastanoi pv. savastanoi isolates and the susceptibility of some native genotypes to olive knot. Area of study: The Aegean, Marmara, and Mediterranean Regions of Turkey. Material and methods: 101 isolated bacteria were identified on the basis of biochemical, PCR for amplification of the bacterial iaaL gene, and pathogenicity tests. The virulence of the isolates was determined in a randomized experimental trial carried out by stem inoculation of pot-grown seedlings of olive (cv. ‘Manzanilla’) in the growing chamber. The amounts of IAA produced by the isolates were determined colorimetrically. The susceptibility of native olive genotypes was evaluated on 2-yr old plants inoculated with two distinct strains. Main results: Tested P. savastanoi pv. savastanoi isolates showed significant differences in virulence found to be associated with their geographical origin. The isolates produced IAA amounts varied from 148.67 to 0.3 μg mL-1. The geographical variation in IAA biosynthesis ability of the isolates was observed. No correlation (R=0.0225) was determined between virulence and IAA amounts of the isolates. Native olive genotypes indicated different susceptibility levels to the olive knot pathogen. No genotype tested had complete resistance. However, low susceptible genotypes (‘Memecik’, ‘Ayvalık’ and ‘Uslu’) were identified. Some genotypes had variable reactions depending on the isolate used. Research highlights: The results undergird the differences in the virulence and IAA production of the isolates within the area and also between geographical locations. Genotypes with low susceptibility can be used as genitors in further breeding studies.

scholarly journals Volatile 1-octanol of tea (Camellia sinensis L.) fuels cell division and indole-3-acetic acid production in phylloplane isolate Pseudomonas sp. NEEL19

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Poovarasan Neelakandan ◽  
Chiu-Chung Young ◽  
Asif Hameed ◽  
Yu-Ning Wang ◽  
Kui-Nuo Chen ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Cell Division ◽  
Petri Dish ◽  
Gas Chromatography Mass Spectrometry ◽  
Dependent Manner ◽  
Tea Leaves ◽  
Acetic Acid Production ◽  
Iaa Production ◽  
Solvent Tolerant ◽  
Indole 3 Acetic Acid

AbstractTea leaves possess numerous volatile organic compounds (VOC) that contribute to tea’s characteristic aroma. Some components of tea VOC were known to exhibit antimicrobial activity; however, their impact on bacteria remains elusive. Here, we showed that the VOC of fresh aqueous tea leaf extract, recovered through hydrodistillation, promoted cell division and tryptophan-dependent indole-3-acetic acid (IAA) production in Pseudomonas sp. NEEL19, a solvent-tolerant isolate of the tea phylloplane. 1-octanol was identified as one of the responsible volatiles stimulating cell division, metabolic change, swimming motility, putative pili/nanowire formation and IAA production, through gas chromatography-mass spectrometry, microscopy and partition petri dish culture analyses. The bacterial metabolic responses including IAA production increased under 1-octanol vapor in a dose-dependent manner, whereas direct-contact in liquid culture failed to elicit such response. Thus, volatile 1-octanol emitting from tea leaves is a potential modulator of cell division, colonization and phytohormone production in NEEL19, possibly influencing the tea aroma.

scholarly journals Highly Efficient Biosynthesis of Indole-3-acetic acid by Enterobacter xiangfangensis BHW6

2020 ◽  
Author(s):  
Bi-Xian Zhang ◽  
Ying-Ying Wang ◽  
Xiaomei Hu
Keyword(s):  
Acetic Acid ◽  
Economic Value ◽  
Pyruvate Decarboxylase ◽  
Genomic Analysis ◽  
Rrna Gene ◽  
H Nmr ◽  
Gene Coding ◽  
Key Enzyme ◽  
Indole 3 Acetic Acid ◽  
Iaa Biosynthesis

Abstract Background: Indole-3-acetic acid (IAA) plays an important role in the growth and development of plants. Various bacteria in the rhizosphere are capable to produce IAA that acts as a signaling molecule for the communication between plants and microbes to promote the plant growth. Due to the low IAA content and various interfering analogs, it is difficult to detect and isolate IAA from microbial secondary metabolites. Results: A predominant strain with a remarkable capability to secrete IAA was identified as Enterobacter xiangfangensis BHW6 based on 16S rRNA gene sequence, the determination of average nucleotide identity (ANI) and digital DDH (dDDH). The maximum IAA content (134-1129 μg/mL) was found with the addition of 0.2-15 g/L of L-tryptophan at pH 5 for 6 days, which was 4-40 fold higher than that in the absence of L-tryptophan. The highest yield of IAA was obtained at the stationary phase of bacterial growth. An acidic culture medium was preferred for the IAA biosynthesis of the strain. The strain was tolerant and stable to produce IAA in the presence 2.5%-5% (w/v) of NaCl. IAA was then isolated through column chromatography with a mobile phase of hexane/ethyl acetate (1/2, v/v) and characterized by 1H Nuclear Magnetic Resonance (1H NMR). Conclusions: A remarkable IAA production was obtained from E. xiangfangensis BHW6 that was tryptophan–dependent. According to genomic analysis, the ipdC gene coding for the key enzyme (indole-3-pyruvate decarboxylase) was identified indicating that IAA biosynthesis was mainly through the indole-3-pyruvia acid (IPyA) pathway, which was further confirmed by intermediate assay. E. xiangfangensis BHW6 with an important economic value has great prospect in agricultural and industrial application.

Characterization of the indole-3-acetic acid (IAA) biosynthetic pathway in an epiphytic strain of Erwinia herbicola and IAA production in vitro

1996 ◽  
Vol 42 (6) ◽  
pp. 586-592 ◽  
Author(s):  
M. Brandi ◽  
E. M. Clark ◽  
S. E. Lindow
Keyword(s):  
Acetic Acid ◽  
Pseudomonas Syringae ◽  
Pyruvic Acid ◽  
Enzyme Assays ◽  
Erwinia Herbicola ◽  
Iaa Production ◽  
Acid Pathway ◽  
Culture Supernatants ◽  
Indole 3 Acetic Acid

An epiphytic strain of Erwinia herbicola (strain 299R) synthesized indole-3-acetic acid (IAA) from indole-3-pyruvic acid and indole-3-acetaldehyde, but not from indole-3-acetamide and other intermediates of various IAA biosynthetic pathways in enzyme assays. TLC, HPLC, and GC–MS analyses revealed the presence of indole-3-pyruvic acid, indole-3-ethanol, and IAA in culture supernatants of strain 299R. Indole-3-acetaldehyde was detected in enzyme assays. Furthermore, strain 299R genomic DNA shared no homology with the iaaM and iaaH genes from Pseudomonas syringae pv. savastanoi, even in Southern hybridizations performed under low-stringency conditions. These observations strongly suggest that unlike gall-forming bacteria which can synthesize IAA by indole-3-acetamide, the indole-3-pyruvic acid pathway is the primary route for IAA biosynthesis in this plant-associated strain. IAA synthesis in tryptophan-supplemented cultures of strain 299R was over 10-fold higher under nitrogen-limiting conditions, indicating a possible role for IAA production by bacterial epiphytes in the acquisition of nutrients during growth in their natural habitat.Key words: indole-3-acetic acid, Erwinia, tryptophan, indole-3-pyruvic acid, nitrogen.

A Prototype Model for Indole-3-Acetic Acid (IAA) Production by Azospirillum brasilense SP245

2004 ◽  
Vol 37 (9) ◽  
pp. 493-498 ◽  
Author(s):  
Ilse Y. Smets ◽  
Kristel Bernaerts ◽  
Astrid Cappuyns ◽  
Ositadinma Ona ◽  
Jos Vanderleyden ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Azospirillum Brasilense ◽  
Prototype Model ◽  
Iaa Production ◽  
Indole 3 Acetic Acid
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