scholarly journals A novel method for chromosome analysis of human sperm using enucleated mouse oocytes

2005 ◽  
Vol 20 (5) ◽  
pp. 1244-1247 ◽  
Author(s):  
Yasuyuki Araki ◽  
Midori Yoshizawa ◽  
Yasuhisa Araki
Keyword(s):  
Chromosome Analysis ◽  
Human Sperm ◽  
Mouse Oocytes ◽  
Novel Method

scholarly journals The Application of Ultrasonic Vibration in Human Sperm Cryopreservation as a Novel Method for the Modification of Physicochemical Characteristics of Freezing Media

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gholami Dariush ◽  
Riazi Gholamhossein ◽  
Fathi Rouhollah ◽  
Ghaffari Seyed Mahmood ◽  
Shahverdi Abdolhossein ◽  
...  
Keyword(s):  
Ultrasonic Vibration ◽  
Human Sperm ◽  
Physicochemical Characteristics ◽  
Sperm Cryopreservation ◽  
Novel Method

scholarly journals A novel method for detection of chromosomal integrity in cryopreserved livestock spermatozoa using artificially fused mouse oocytes

2010 ◽  
Vol 27 (9-10) ◽  
pp. 581-588 ◽  
Author(s):  
Hiroyuki Watanabe ◽  
Hiroshi Suzuki ◽  
Hiroyuki Tateno ◽  
Yutaka Fukui
Keyword(s):  
Mouse Oocytes ◽  
Novel Method

Chromosome analysis of human sperm

1987 ◽  
Vol 76 (4) ◽  
pp. 385-388 ◽  
Author(s):  
J. Jenderny ◽  
G. R�hrborn
Keyword(s):  
Chromosome Analysis ◽  
Human Sperm

scholarly journals Fertilizability and chromosomal integrity of frozen-thawed Bryde's whale (Balaenoptera edeni) spermatozoa intracytoplasmically injected into mouse oocytes

Zygote ◽  
2007 ◽  
Vol 15 (1) ◽  
pp. 9-14 ◽  
Author(s):  
H. Watanabe ◽  
H. Tateno ◽  
H. Kusakabe ◽  
T. Matsuoka ◽  
Y. Kamiguchi ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Chromosome Aberrations ◽  
Chromosome Complement ◽  
Chromosome Analysis ◽  
Oocyte Activation ◽  
Mouse Oocytes ◽  
Structural Chromosome ◽  
Sperm Nuclei ◽  
Immotile Spermatozoa

SUMMARYPrior to attempting the in vitro production of embryos in the Bryde's whale (Balaenoputera edeni), we investigated whether spermatozoa can retain the capacity for oocyte activation and pronucleus formation as well as chromosomal integrity under cryopreservation by using intracytoplasmic sperm injection (ICSI) into mouse oocytes. Regardless of motility and viability, whale spermatozoa efficiently led to the activation of mouse oocytes (90.3–97.4%), and sperm nuclei successfully transformed into male pronucleus within activated ooplasm (87.2–93.6%). Chromosome analysis at the first cleavage metaphase (M) of the hybrid zygotes revealed that a majority (95.2%) of motile spermatozoa had the normal chromosome complement, while the percentage of chromosomal normality was significantly reduced to 63.5% in immotile spermatozoa and 50.0% in dead spermatozoa due to the increase in structural chromosome aberrations. This is the first report showing that motile Bryde's whale spermatozoa are competent to support embryonic development.

scholarly journals Pronuclear epigenetic modification of protamine deficient human sperm following injection into mouse oocytes

2016 ◽  
Vol 62 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Hoda Rajabi ◽  
Homa Mohseni-Kouchesfehani ◽  
Abdollah Mohammadi-Sangcheshmeh ◽  
Fattaneh Farifteh-Nobijari ◽  
Mohammad Salehi
Keyword(s):  
Epigenetic Modification ◽  
Human Sperm ◽  
Mouse Oocytes
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