Human sperm chromosome analysis as a mutagenicity test of chemicals

1993 ◽  
Vol 292 (3) ◽  
pp. 273
Author(s):  
Y. Kamiguchi ◽  
Y. Iizawa ◽  
H. Tateno ◽  
K. Mikamo
Keyword(s):  
Chromosome Analysis ◽  
Human Sperm ◽  
Mutagenicity Test

Chromosome analysis of human sperm

1987 ◽  
Vol 76 (4) ◽  
pp. 385-388 ◽  
Author(s):  
J. Jenderny ◽  
G. R�hrborn
Keyword(s):  
Chromosome Analysis ◽  
Human Sperm

scholarly journals A novel method for chromosome analysis of human sperm using enucleated mouse oocytes

2005 ◽  
Vol 20 (5) ◽  
pp. 1244-1247 ◽  
Author(s):  
Yasuyuki Araki ◽  
Midori Yoshizawa ◽  
Yasuhisa Araki
Keyword(s):  
Chromosome Analysis ◽  
Human Sperm ◽  
Mouse Oocytes ◽  
Novel Method

Human sperm chromosome analysis after microinjection into hamster oocytes

1995 ◽  
Vol 12 (6) ◽  
pp. 384-388 ◽  
Author(s):  
J. Cozzi ◽  
E. Chevret ◽  
S. Rousseaux ◽  
R. Pelletier ◽  
B. Sèle
Keyword(s):  
Chromosome Analysis ◽  
Human Sperm

A High Resolution Cytochemical Study of Nuclear ATPase in Human Spermatozoa

1975 ◽  
Vol 33 ◽  
pp. 594-595
Author(s):  
A. Sosa ◽  
L. Calzada
Keyword(s):  
High Resolution ◽  
Atpase Activity ◽  
Nuclear Membrane ◽  
Human Sperm ◽  
Sperm Nucleus ◽  
Human Spermatozoa ◽  
Cytochemical Study ◽  
Membrane Bound ◽  
Sperm Nuclei ◽  
Interchromatin Space

The dependence of nuclear metabolism on the function of the nuclear membrane is not well understood. Whether or not the function of the nuclear membrane is partial or totally responsible of the repressed template activity of human sperm nucleus has not at present been elucidated. One of the membrane-bound enzymatic activities which is concerned with the mechanisms whereby substances are thought to cross cell membranes is adenosintriphosphatase (ATPase). This prompted its characterization and distribution by high resolution photogrammetry on isolated human sperm nuclei. Isolated human spermatozoa nuclei were obtained as previously described. ATPase activity was demonstrated by the method of Wachstein and Meisel modified by Marchesi and Palade. ATPase activity was identified as dense and irregularly distributed granules confined to the internal leaflet of the nuclear membrane. Within the nucleus the appearance of the reaction product occurs as homogenous and dense precipitates in the interchromatin space.

Sign in / Sign up

Export Citation Format

Share Document