Five-Year Study of Mycotoxins in Virginia Wheat and Dent Corn

1983 ◽  
Vol 66 (6) ◽  
pp. 1466-1469 ◽  
Author(s):  
Odette L Shotwell ◽  
Clifford W Hesseltine

Abstract Every year during the 5-year period 1976-1980, approximately 100 samples each of corn and wheat from trucks delivering the grains at elevators in Virginia were collected by personnel of the Federal Grain Inspection Service and shipped to NRRC. Samples were analyzed as soon as possible for aflatoxin, zearalenone, and ochratoxin A. The 3 mycotoxins were not detected in any wheat sample. Zearalenone and ochratoxin A were not found in any corn sample; however, aflatoxin was detected in at least 25% of the corn samples from every crop year. In 1976-1980, the incidence of aflatoxin at levels of 20 ng/g or more (the Food and Drug Administration guideline) ranged from 18 to 61%; aflatoxin incidence above 100 ng/g was 5-29%. The average aflatoxin levels in corn samples collected in the 5 years varied from 21 to 137 ng/g. Moisture content of the samples was not determined, so aflatoxin levels given may be higher than they were at harvest. However, there are obviously differences from year to year. In freshly harvested corn samples collected by fieldmen of the Statistical Reporting Service in yield surveys in 1978 and 1979, aflatoxin incidence above the FDA guideline was 10 and 13%, and above 100 ng/g was 4 and 7%. The average aflatoxin level in all samples collected in 1978 was 13 ng/g and in 1979, 36 ng/g. Some aflatoxin can be expected yearly in Virginia corn, but the incidence and levels vary from year to year.

1995 ◽  
Vol 78 (3) ◽  
pp. 631-636 ◽  
Author(s):  
Mary W Trucksess ◽  
Frederick Thomas ◽  
Kathryn Young ◽  
Michael E Stack ◽  
Wendy J Fulgueras ◽  
...  

Abstract Wheat and barley from the 1993 crop year were analyzed for deoxynivalenol (DON). A total of 630 samples were collected by the Federal Grain Inspection Service in 25 states and analyzed using a commercially available, direct competitive, enzyme-linked immunosorbent assay. The limit of determination was about 0.5 μg/g. DON contamination in the 483 wheat samples averaged 2.0 μg/g and ranged from <0.5 to 18 μg/g. DON contamination in the 147 barley samples averaged 4.2 μg/g and ranged from <0.5 to 26 μg/g. About 40% of the wheat samples and 57% of the barley samples contained DON levels that were greater than the U.S. Food and Drug Administration 1982 advisory level of 2 μg/g for DON in wheat designated for milling (human consumption).


2018 ◽  
Vol 81 (7) ◽  
pp. 1063-1067 ◽  
Author(s):  
ROBERT G. REINHARD ◽  
ROBIN M. KALINOWSKI ◽  
PETER W. BODNARUK ◽  
JOSEPH D. EIFERT ◽  
RENEE R. BOYER ◽  
...  

ABSTRACT A multiyear survey of 31 ready-to-eat (RTE) food processing plants in the United States was conducted to determine the incidence of Listeria spp. in various RTE production environments. Samples were collected from 22 RTE plants regulated by the U.S. Department of Agriculture's Food Safety and Inspection Service (FSIS) and from 9 RTE food plants regulated by the U.S. Department of Health and Human Services' Food and Drug Administration (FDA). Only nonfood contact surfaces in the RTE manufacturing areas with exposed RTE product were sampled. Each sample was individually analyzed for the presence of Listeria spp. by using a PCR-based rapid assay. In total, 4,829 samples were collected from various locations, including freezers, equipment framework, floors, walls, wall-floor junctures, drains, floor mats, doors, and cleaning tools. Nine (29%) of the facilities had zero samples positive for Listeria spp. in the production environment, whereas 22 (71%) had one or more samples positive for Listeria spp. The total incidence of Listeria spp. in all RTE food plants was 4.5%. The positive rate in plants regulated by the FSIS ranged from 0 to 9.7%, whereas the positive rate in plants regulated by the FDA ranged from 1.2 to 36%.


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