Fermentation time difference of nixtamalized horse dent corn (Zea mays var. indenata) by Bifidobacterium bifidum and Bifidobacterium brevis as source of natural folic acid

Bifidobacterium sp. as microbes has potential role in fermentation of nixtamalized horse dent corn (Zea mays var. indentata) to degrade complex components into folic acid-rich corn biomass. Fermentation process on both nixtamalized yellow corn and white corn by Bifidobacterium brevis and Bifidobacterium bifidum as substrat of A, B, C and D were conducted at concentration of corn folic acid inoculum 40% (w/w) and 37 °C for 0, 8, 16, and 24 hours, respectively. Based on dissolved protein yielded, the experiment result showed that the best result of optimization in fermentation of both nixtamalized yellow corn (biomass B) and white corn (biomass D) was achieved by using inoculum of B. bifidum for 16 hours with composition of folic acid of 213.58 and 297.72 μg/mL, total solids of 21.14 and 21.07%, dissolved protein of 0.42 and 0.39 mg/mL, reducing sugars of 34.2 and 37.8 mg/mL, total sugars of 104.7 and 98.6 mg/mL, total acids of 0.37 and 0.44%, N-amino of 0.28 and 0.26 mg/g, and pH 4.82 and 4.49, respectively. In this condition, biomass of B. and biomass of D indicated domination of folic acid monomer with molecular weight (MW) 442.29 and 442.59 Dalton (Da.) at relative intensity 100%, particles size of 1115.1 nm and 1075.7 nm, and particle index of 0.827 and 0.849, respectively. Meanwhile, volatile compounds were dominated by 2,3-butanediol of 4.46 and 10.65%, palmitic acid of 7.63 and 8.26%, octadecenoic acid of 6.31 and 9.5%, lactic acid of 2.37% and 0.53%, respectively.

The influence of particle size of Enogen Feed corn and conventional yellow dent corn on nursery and finishing pig performance, carcass characteristics and stomach morphology

Enogen Feed corn is a variety developed by Syngenta Seeds (Downers Grove, IL) that has been genetically modified to contain an α-amylase enzyme trait (SYT-EFC). Originally, Enogen feed corn was developed for the ethanol industry due to its reduction in viscosity of the corn mash, thus eliminating the need to add a liquid form of the α-amylase enzyme. However, there is potential application for Enogen Feed corn to be used in livestock diets due to the increase in α-amylase enzyme potential to increase starch digestibility. A more common method of increasing starch digestibility in corn is to finely grind it to reduce particle size. This increases the surface area and allows for greater interaction with digestive enzymes. We hypothesized that pigs fed Enogen feed corn potentially could achieve similar gain:feed ratio (G:F) at larger particle sizes than conventional corn because of the differences in starch digestibility. In Exp. 1, a total of 360 pigs (DNA 200 × 400, Columbus, NE; initially 6.6 ± 0.1 kg BW) were used with 5 pigs per pen and 12 pens per treatment. Treatments were arranged in a 2 × 3 factorial with main effects of corn source (Enogen Feed corn or conventional yellow dent corn) and ground corn particle size (300, 600, or 900 µm). Overall, there was a corn source × particle size interaction (linear, P = 0.027) for G:F. There was no effect due to particle size when pigs were fed conventional yellow dent corn, but in pigs fed Enogen Feed corn, G:F increased with decreasing particle size. Neither corn source nor particle size affected (P > 0.05) overall average daily gain (ADG) or average daily feed intake (ADFI). In Exp. 2, a total of 323 pigs (241 × 600; DNA, Columbus, NE; initially 50.0 ± 1.3 kg) were used with 9 pigs per pen and 6 pens per treatment. Treatments were identical as Exp. 1. Overall, corn source had no effect on finishing pig ADG, ADFI or G:F. For corn particle size, ADG and G:F increased (linear, P < 0.014) and ADFI decreased (P = 0.043) as particle size decreased. For stomach morphology, there was a tendency for a corn source × particle size interaction (P = 0.055) for keratinization score with keratinization increasing linearly (P = 0.001) as particle size of the corn decreased for yellow dent corn with no change in keratinization score as particle size decreased for Enogen Feed corn. In summary, reducing corn particle size improved G:F with no major differences observed between corn sources for overall pig performance.

ALIH TEKNOLOGI PENGOLAHAN PANGAN LOKAL DI KENAGARIAN ANDALEH, LIMAPULUH KOTA

Jagung banyak dibudidayakan di Kenagarian Andaleh di Kecamatan Luak, Kabupaten Limapuluh Kota. Jenis jagung yang dibudidayakan adalah jagung gigi kuda yang sering dijadikan sebagai pakan ternak terutama pakan unggas. Jagung gigi kuda memiliki biji berbentuk gigi, mengandung karbohidrat (pati) yang tinggi, dan bertekstur keras. Ciri khas jagung ini adalah memiliki biji yang melekuk di bagian tengah atau bagian atas biji. Kegiatan ini bertujuan untuk meningkatkan pengetahuan masayarakat Nagari Andaleh Kecamatan Luak, tentang pemanfaatan bahan pangan lokal, khususnya komoditi jagung ini melalui alih teknologi pengolahan menjadi nugget jagung. Kegiatan diawali dengan memberikan penyuluhan tentang praktik sanitasi, meliputi sanitasi pekerja, sanitasi peralatan, dan sanitasi pengolahan, serta cara pengolahan pangan yang baik. Nugget jagung dibuat dari campuran daging ayam dan jagung dengan perbandingan 1:0.2. Melalui kegiatan ini, masyarakat mampu menerapkan higieni dan sanitasi dalam pengolahan pangan, serta memiliki keahlian dalam pengolahan jagung menjadi produk pangan baru, yaitu nugget jagung, sehingga meningkatkan pemanfaatan bahan pangan lokal dan meningkatkan perekonomian masyarakat. Dengan alih teknologi pemanfaatan bahan pangan lokal jagung diharapkan tumbuh motivasi untuk mengembangkan usaha skala rumah tangga yang berpengaruh pada peningkatan perekonomian masyarakat di Kenagarian Andaleh. Kata kunci: Jagung Gigi Kuda, Nagari Andaleh, Nugget Jagung, Alih Teknologi, Pangan Lokal ABSTRACT Corn is widely cultivated in Andaleh Village, Luak Sub-district, Lima Puluh Kota Regency. The type of corn that cultivated in this village is dent corn (Zea mays var. indentata), which often used as animal feed, especially poultry feed. Dent corn has a tooth-shaped seed, contains high carbohydrates (starch), and has a hard texture. The characteristic of this corn form is that the seeds have an indentation in the middle or top of the seed. The purpose of this programs was to increased the knowledge Andaleh Village community, Luak District, about the utilization of local food, especially dent corn through the transition technology of corn processing to be corn nuggets. The activity began with provided counseling on sanitation practices, including personal sanitation, equipment sanitation, and processing sanitation, as well as good manufacturing practice. Corn nuggets were made from a mixture of chicken and corn in a ratio of 1: 0.2. Through this programs, the community will be able to apply hygiene and sanitation in food processing, and have expertise in processing corn into new products like nuggets, thereby can increase the utilization of local foodstuffs and improve the community's economy. Transition technology to use local dent corn was expected to grow the motivation of community to develop household-scale businesses that have an effect to increase the economy of the Andaleh Village community. Keywords: Dent Corn, Andaleh Village, Corn Nugget, Transition of Technology, Local Foods

Influence of Enogen Feed corn and conventional yellow dent corn in pelleted or meal-based diets on finishing pig performance and carcass characteristics

Genetic modification of corn has enhanced the use of different corn hybrids in animal agriculture. Enogen Feed corn, developed by Syngenta Seeds (Downers Grove, IL), has potential for use in livestock diets due to increase α-amylase enzyme in the corn thus improving starch digestibility. In addition, the pelleting process also increases starch gelatinization which increases its digestibility by the pig, increasing growth rate and improving feed efficiency. Therefore, pelleting Enogen Feed corn might prove to provide a greater response in growth performance than conventional yellow dent corn. Thus, the objective of this experiment was to determine the effects of corn source and diet form on growth performance and carcass characteristics of finishing pigs. A total of 288 pigs (53.0 ± 0.5 kg) were used with 8 pigs per pen and 9 pens per treatment in a 72-d study. Treatments were arranged in a 2 × 2 factorial with main effects of corn source (Enogen Feed corn or conventional yellow dent corn) and diet form (meal or pellet). For overall (d 0 to 72) performance, no interactions between corn source and diet form were observed. There was a tendency (P < 0.10) for slightly improved average daily gain (ADG) and gain:feed ratio (G:F) for pigs fed conventional yellow dent corn compared to those fed Enogen Feed corn. For feed form, pigs fed pelleted diets had increased (P < 0.001) ADG and G:F compared to pigs fed meal diets. For carcass characteristics, pigs fed pelleted diets had increased hot carcass weight compared to pigs fed meal diets (P < 0.001). In summary, feeding pelleted diets to finishing pigs increased ADG and improved feed efficiency compared to those fed meal-based diets. There were no major differences between observed corn sources or interactions between corn source and diet form on growth performance.

140 Influence of Enogen Feed Corn and Conventional Yellow Dent Corn in Pelleted or Meal-based Diets on Finishing Pig Performance and Carcass Characteristics

Previous research has indicated that starch gelatinization during the pelleting process is greater for Enogen® Feed corn compared to conventional yellow dent corn. Increasing starch gelatinization in the pellet increases the starch digestibility in the pig, which potentially leads to increased growth rate. Therefore, the objective of this study was to determine the effects of feeding Enogen Feed corn in meal or pellet form on finishing pig growth performance and carcass characteristics. A total of 288 pigs (53.0 ± 0.5 kg) were used with 8 pigs/pen and 9 pens/treatment in a 72-d study. Treatments were arranged in a 2×2 factorial with main effects of corn source (Enogen Feed corn or conventional yellow dent corn) and diet form (meal or pellet). Main effects of corn source and diet form as well as their interactions were tested. Pelleting parameters were established with a target conditioner temperature of 82.2°C and corn moisture of 13 to 14%. When pelleting the diets, the conditioning temperature for conventional yellow dent corn averaged 68.4°C and Enogen Feed corn averaged 67.7°C. The hot pellet temperature for conventional yellow dent corn averaged 75.1°C and 75.8°C for Enogen feed corn. For overall performance (d 0 to 72), no interactions between corn source and diet form were observed (P > 0.05). There was a tendency (P < 0.10) for slightly improved average daily gain (ADG) and gain:feed ratio (G:F) for pigs fed conventional yellow dent corn compared to those fed Enogen Feed corn. Pigs fed pelleted diets had increased (P < 0.001) ADG, G:F, and hot carcass weight compared to pigs fed meal diets. In summary, feeding pelleted diets to finishing pigs increased ADG and G:F compared to those fed meal-based diets. There were no major differences observed between corn sources or interactions between corn source and diet form on growth performance.

133 Influence of Particle Size of Enogen Feed Corn and Conventional Yellow Dent Corn on Nursery and Finishing Pig Performance, Carcass Characteristics and Stomach Morphology

Two studies evaluated the effect of particle size of Enogen® Feed corn (Syngenta Seeds, LLC, Downers Grove, IL) and conventional yellow dent corn on nursery and finishing pig performance, carcass characteristics and stomach morphology. In Exp. 1, 360 nursery pigs (DNA 200×400, Columbus, NE; initially 6.6±0.1 kg BW) were used with 5 pigs per pen and 12 pens per treatment. Treatments were arranged in a 2×3 factorial with main effects of corn source (Enogen Feed corn or conventional yellow dent corn) and ground corn particle size (300, 600, or 900 µm). Overall, there was a corn source×particle size interaction (linear, P = 0.027) for G:F ratio. There was no difference due to particle size when pigs were fed conventional yellow dent corn, but in pigs fed Enogen Feed corn, G:F increased with decreasing particle size. Neither corn source nor particle size affected (P > 0.05) ADG or ADFI. In Exp. 2, 323 finishing pigs (241′600; DNA, Columbus, NE; initially 50.0±1.3 kg) were used with 8 or 9 pigs per pen and 6 pens per treatment. Treatments were arranged identical to Exp. 1. Overall, corn source did not elicit differences in ADG, ADFI or G:F (P > 0.05). For corn particle size, ADG and G:F increased (linear, P ≤ 0.014) and ADFI decreased (P = 0.043) as particle size decreased. For carcass characteristics, there was a tendency (linear, P = 0.093) for increased HCW and increased (linear, P = 0.023) carcass yield as corn particle size decreased. For stomach morphology, there was a tendency for a corn source×particle size interaction (P = 0.055) for keratinization score with keratinization increasing linearly (P = 0.001) as particle size decreased for yellow dent corn with no change in keratinization score as particle size decreased for Enogen Feed corn. In summary, reducing corn particle size improved G:F with no major differences observed between corn sources for overall pig performance.

PSIV-15 Influence of Particle Size of Enogen Feed Corn and Conventional Yellow Dent Corn on Lactating Sow Performance

A total of 107 sows (Line 241; DNA, Columbus, NE) across 4 batch farrowing groups were used to evaluate the effects of corn source and particle size on sow and litter performance. Treatments were arranged in a 2×2 factorial with main effects of corn source (Enogen® Feed corn (Syngenta Seeds, Downers Grove, IL) or conventional yellow dent corn) and ground corn particle size (600 or 900 µm). Sows were blocked by parity and BW upon arrival to the farrowing house. There were approximately 27 sows per treatment, sow was considered the experimental unit, dietary treatment was a fixed effect, and sow group and block were used as random effects. Main effects of corn source and particle size as well as their interactions were tested. From farrowing to weaning, there was a tendency for a source×particle size interaction (P=0.065) for sow BW change. Sows fed 900 µm Enogen Feed corn had decreased BW loss compared to sows fed other treatments which were similar in BW loss. There was a source×particle size interaction (P=0.048) for lactation ADFI with sows fed 900 µm conventional yellow dent corn having lower feed intake than the sows fed 600 µm conventional yellow dent corn, whereas sows fed 900 µm Enogen Feed corn had greater feed intake compared to the sows fed 600 µm Enogen Feed corn. There was a tendency for a particle size main effect (P<0.10) for litter ADG (2,849 vs 2,635 g/d) and total litter gain (45.7 vs 42.3 kg), with sows fed corn ground to 600 µm having increased litter ADG and total litter gain compared to sows fed corn ground to 900 µm. In summary, there were few differences in sow or litter characteristics among corn sources. Reducing particle size of both corn sources tended to increase litter ADG and weaning weights.

Influence of particle size of Enogen Feed corn and conventional yellow dent corn on lactating sow performance

Enogen Feed corn is a variety developed by Syngenta Seeds (Downers Grove, IL) that has been genetically modified to contain an α-amylase enzyme trait (SYT-EFC). Originally, Enogen feed corn was developed for the ethanol industry, due to its properties for reducing the viscosity of its corn mash. There is potential application for Enogen Feed corn to be used in livestock diets due to the potential for the increase in α-amylase enzyme to increase the starch digestibility. Because of this, it may be possible to increase particle size of ground Enogen Feed corn and maintain the same starch digestibility as finely ground conventional yellow dent corn. Therefore, our hypothesis was that an interaction between corn source and particle size would exist, such that performance of sows fed fine ground conventional yellow dent corn would be similar to sows fed coarse ground Enogen Feed corn. A total of 107 sows (Line 241; DNA, Columbus, NE) across 4 batch farrowing groups were used to evaluate sow and litter performance. Treatments were arranged in a 2 × 2 factorial with main effects of corn source (Enogen Feed corn or conventional yellow dent corn) and ground corn particle size (600 or 900 µm). From farrowing to weaning, there was a tendency for a corn source × particle size interaction (P = 0.065) in sow body weight change. Sows fed 900 µm Enogen Feed corn had decreased body weight loss compared to sows fed other treatments which were similar in weight loss. For sow ADFI from farrowing to weaning, there was a corn source × particle size interaction (P = 0.048) with sows fed 900 µm conventional yellow dent corn having lower feed intake than the sows fed 600 µm conventional yellow dent corn, whereas sows fed 900 µm Enogen Feed corn had greater feed intake compared to the sows fed 600 µm Enogen Feed corn. There was a tendency for a particle size main effect (P < 0.10) for litter ADG and total litter gain, with sows fed corn ground to 600 µm having increased litter ADG and total litter gain compared to sows fed corn ground to 900 µm. In summary, there were few differences in sow or litter characteristics among those fed Enogen Feed corn or conventional yellow dent corn. Reducing particle size of both corn sources tended to increase litter ADG and weaning weights.

Effects of exogenous α-amylases, glucoamylases and proteases on ruminal in vitro dry matter and starch digestibility, gas production and volatile fatty acids of mature dent corn grain

Two separate experiments were carried out to evaluate the effects of incremental doses of 10 exogenous endo-acting α-amylase and exo-acting glucoamylase; 1LAT (bacterial α-amylase), 2AK, 3AC, 4Cs4, 5Trga, 6Afuga, 7Fvga, and 10Tg (fungal α-amylases, glucoamylases and α-glucosidase), 8Star and 9Syn (fungal amylase-mixtures; Exp. 1) and 3 exogenous proteases; 11P14L, 12P7L, and 13P30L (bacterial proteases; Exp. 2) on in vitro dry matter digestibility (IVDMD) and in vitro starch digestibility (IVSD) of mature dent corn grain using a batch culture system. Incremental doses of the exogenous enzymes (0, 0.25, 0.50, 0.75 and 1.00 mg/g of dried substrate) were applied directly to the substrate (0.5 g of ground corn, 4 mm) in sextuplicate (Exp. 1) or quadruplicate (Exp. 2) within F57 filter bags, which were incubated at 39°C in buffered rumen fluid for 7 h. Rumen fluid was collected 2-3 h after the morning feeding from 3 lactating dairy cows and pooled. Cows were consuming a mid-lactation total mixed ration (TMR; 1.60 Mcal/kg DM and 15.4%; net energy of lactation and crude protein, respectively). Three independent runs were carried out for each experiment. Data were analyzed as a randomized complete block design using run as the blocking factor. Dose was used as a fixed factor while run was considered a random factor. Linear, quadratic, and cubic orthogonal contrasts were also tested. In Exp. 1, enzymes 2AK, 3AC and 10Tg did not increase (P > 0.10) IVDMD and IVSD, whereas 0.25 mg of enzymes 1LAT, 5Trga and 8Star increased (P < 0.01) IVDMD by 23, 47 and 62% and IVSD by 35, 41 and 58%, respectively, compared with the control. Enzymes 4Cs4, 6Afuga, 7Fvga and 9Syn linearly increased IVDMD and IVSD (P < 0.01). Greatest increases in IVDMD (82.9%) and IVSD (85.9%) resulted with 1 mg of 6Afuga compared to control. In Exp. 2, the lowest dose of exogenous proteases 11P14L and 12P7L increased (P < 0.01) IVDMD by 98 and 87% and IVSD by 57 and 64%, respectively, whereas the highest dose of 13P30L increased (P = 0.02) IVDMD by 44.8% and IVSD by 30%, relative to the control. In conclusion, IVSD and IVDMD were increased by one α-amylase, certain glucoamylases and all proteases tested, with the glucoamylase 6Afuga in Exp. 1 and the neutral protease12P7L in Exp. 2, increasing IVDMD and IVSD to the greater extents. Future in vivo studies are required to validate these findings before these enzyme additives can be recommended for improving the digestibility of mature dent corn grain.

PSVIII-1 Determination of in vitro DM, N and NDF digestibility and fermentability in novel corn co-products for swine

New process technologies are being implemented in the ethanol industry resulting in new high protein corn co-products (HPCP) containing more gross energy and amino acids than conventional distillers dried grains with solubles (DDGS). Thus, estimates of nutrient digestibility for HPCP are needed to assess potential feeding value for swine. Furthermore, yellow dent corn is the predominant grain source in U.S. ethanol and co-product production whereas flint corn is used in Brazil. This study determined and compared in vitro dry matter (DM), nitrogen (N), and neutral detergent fiber (NDF) digestibility and fermentability of U.S. and Brazil corn DDGS and new HPCP. In vitro methods evaluated DM, N, and NDF digestibility and fermentability of 2 corn DDGS samples from U.S. (POET and ICM), 2 DDGS samples from Brazil (BR1 and BR2), 1 corn fiber and solubles sample (CF+S), 3 high protein distillers grains (HP-DDG) samples (BR-HP, US-HP, US-HPG1.5), and 2 HP-DDG samples containing high yeast (Ultramax; UM) and StillPro (SP). Digestibility of DM from hydrolysis varied and was greatest for UM (87.13%; P < 0.001) compared with all other co-products. Fermentability of DM was greater (P < 0.001), and similar for UM (85.6%), SP (76.8%), and US-HP-DDG (72.8%) compared with other co-products. Digestibility of N was greater for UM (91.2%; P < 0.001) compared with all other co-products. NDF digestibility was greater (P < 0.001) for UM (62.5%) and SP (60.5%) compared with all other co-products. POET and ICM DDGS had greater (P < 0.001) DM hydrolysis (78.7% and 68.2%, respectively), than BR1 (37.2%) and BR2 (35.0%) and N digestibility (POET = 65.0%; ICM = 58.9%) compared with BR1 (54.4%) and BR2 (43.4%). These results suggest greater nutritional value for Ultramax and StillPro than conventional DDGS and HP-DDG sources (U.S. and Brazilian) due to greater DM, N, and NDF digestibility.