scholarly journals PSXIV-42 Late-Breaking: Effects of yeast cell wall product on growth performance, immune responses, and gene expression of tight junction proteins of weaned pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 333-334
Author(s):  
Hyunjin Kyoung ◽  
Myungwoo Cho ◽  
Hanbae Lee ◽  
Sangwoo Park ◽  
Joowon Kang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Immune Responses ◽  
Tight Junction ◽  
Yeast Cell ◽  
Growth Performance ◽  
Experimental Period ◽  
Yeast Cell Wall ◽  
Weaned Pigs ◽  
Tnf Α

Abstract The study was conducted to investigate effects of yeast cell wall product on growth performance, immune responses, and gene expression of tight junction proteins of weaned pigs. A total of 112 weaned pigs (7.98 ± 0.43 kg BW) were randomly assigned to 2 dietary treatments (8 pigs/pen; 7 replicates/treatments) for 4 weeks in a randomized complete block design (block = BW). Dietary treatments were 1) a commercial basal nursery diet (CON) and 2) CON + 0.05% yeast cell wall product (YCW, EasyBio Inc., Seoul, Korea). Blood was collected from one randomly selected pig per pen on d 0, 7, and 14 after weaning. The randomly selected one pig per replicate was euthanized to collect ileum tissue samples at the end of the experimental period. Measurements were growth performance, number of white blood cells (WBC) by an automated hematology analyzer calibrated for porcine blood, cortisol, tumor necrosis factor-α (TNF-α), transforming growth factor-β1, interleukin-1β (IL-1β), and interleukin-6 (IL-6) by the ELISA, and gene expression of tight junction in ileum tissues. Data were analyzed using the PROC GLM procedure of SAS. The statistical model for every measurement included dietary effect and BW as a covariate. Pigs fed YCW had higher (P < 0.10) ADG than those fed CON during overall experimental period. Pigs fed YCW had lower WBC on d 14 (P < 0.10), TNF-α on d 7 (P < 0.10), and decreased IL-1β on d 14 (P < 0.05) than those fed CON. In addition, The YCW increased (P < 0.05) expression of Claudin family, Occludin, Muc1, INF-α, and IL-6, but decreased (P < 0.05) expression of TNF-α genes in the ileum tissues compared with CON. In conclusion, addition of yeast cell wall product in the nursery diet improved growth performance and gut health and modified immune responses of weaned pigs.

scholarly journals Dietary Yeast Cell Wall Improves Growth Performance and Prevents of Diarrhea of Weaned Pigs by Enhancing Gut Health and Anti-Inflammatory Immune Responses

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2269
Author(s):  
Jeong Jae Lee ◽  
Hyunjin Kyoung ◽  
Jin Ho Cho ◽  
Jeehwan Choe ◽  
Younghoon Kim ◽  
...  
Keyword(s):  
Cell Wall ◽  
Body Weight ◽  
Immune Responses ◽  
Yeast Cell ◽  
Growth Performance ◽  
Relative Abundance ◽  
Yeast Cell Wall ◽  
Intestinal Health ◽  
Weaned Pigs ◽  
Tnf Α

Dietary yeast cell wall products (YCW) are recognized as a feed additive due to multifunctional benefits by the biological response modulators. Thus, this study was conducted to verify a potential advantage of YCW for improving growth performance, nutrient digestibility, immune responses, and intestinal health and microbiota of weaned pigs. A total of 112 weaned pigs (7.99 ± 1.10 kg of body weight; 28 days old) were arbitrarily allocated to two experimental treatments with eight pigs (four barrows and four gilts) per pen and seven replicate pens per treatment in a completely randomized block design (block = BW and sex): (1) a basal diet based on corn and soybean meal (CON) and (2) CON + 0.05% YCW. The experimental period was for 4 weeks. There were no differences in final body weight, average daily feed intake, and gain-to-feed ratio between dietary treatments. In contrast, pigs fed YCW had higher average daily gain (p = 0.088) and apparent ileal digestibility of DM (p < 0.05) and energy (p = 0.052) and lower diarrhea frequency (p = 0.083) than those fed control diet (CON). Pigs fed YCW also had a higher (p < 0.05) ratio between villus height and crypt depth, villus width and area, and goblet cell counts in the duodenum and/or jejunum than those fed CON. Dietary YCW decreased (p < 0.05) serum TNF-α and IL–1β of weaned pigs on day 7 and 14, respectively, compared with CON. Furthermore, pigs fed YCW had higher (p < 0.05) ileal gene expression of claudin family, occludin, MUC1, INF-γ, and IL-6 and lower (p < 0.05) that of TNF-α than those fed CON. Lastly, there were no differences in the relative abundance of bacteria at the phylum level between CON and YCW. However, dietary YCW increased (p < 0.05) the relative abundance of genera Prevotella and Roseburia compared with CON. This study provided that dietary YCW improved growth rate, nutritional digestibility, and intestinal health and modified immune responses and intestinal microbiota of weaned pigs.

scholarly journals Dietary Glutamic Acid Modulates Immune Responses and Gut Health of Weaned Pigs

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 504
Author(s):  
Hyunjin Kyoung ◽  
Jeong Jae Lee ◽  
Jin Ho Cho ◽  
Jeehwan Choe ◽  
Joowon Kang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Immune Responses ◽  
Glutamic Acid ◽  
Growth Performance ◽  
Experimental Period ◽  
Nutrient Digestibility ◽  
Intestinal Morphology ◽  
Weaned Pigs ◽  
Tnf Α

Dietary glutamic acid (GLU) is used as a feed additive because of its functional characteristics that may affect the growth performance and health of pigs. This study was carried out to determine the effects of dietary GLU on growth performance, nutrient digestibility, immune responses, and intestinal health of weaned pigs. A total of ninety-six weaned pigs (8.07 ± 1.17 kg of body weight; 28 days of age) were assigned to two dietary treatments (8 pigs/pen; 6 replicates/treatment) in a randomized complete block design (block: body weight): (1) a typical weaner diet (CON) and (2) CON supplemented with 0.5% GLU. The experimental period was for 4 weeks. All data and sample collections were performed at the specific time points during the experimental period. Pigs fed GLU had higher average daily gain and average daily feed intake for the first two weeks and nutrient digestibility than pigs fed CON. In addition, dietary GLU increased villus height to crypt depth ratio, number of goblet cells, and ileal gene expression of claudin family and occludin compared with CON, but decreased serum TNF-α and IL-6 and ileal gene expression of TNF-α. Moreover, pigs fed GLU had increased relative composition of bacterial communities of genus Prevotella and Anaerovibrio and decreased genus Clostridium and Terrisporobacter compared with those fed CON. This study suggests that dietary GLU influences growth performance and health of weaned pigs by modulating nutrient digestibility, intestinal morphology, ileal gene expression of tight junction proteins and cytokines, immune responses, and microbial community in the gut.

scholarly journals Investigation of the Efficacy of a Postbiotic Yeast Cell Wall-Based Blend on Newly-Weaned Pigs under a Dietary Challenge of Multiple Mycotoxins with Emphasis on Deoxynivalenol

Toxins ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 504
Author(s):  
Debora Muratori Holanda ◽  
Alexandros Yiannikouris ◽  
Sung Woo Kim
Keyword(s):  
Cell Wall ◽  
Body Weight ◽  
Yeast Cell ◽  
Growth Performance ◽  
Block Design ◽  
Nutrient Digestibility ◽  
Yeast Cell Wall ◽  
Jejunal Mucosa ◽  
Weaned Pigs ◽  
Two Factors

Pigs are highly susceptible to mycotoxins. This study investigated the effects of a postbiotic yeast cell wall-based blend (PYCW; Nicholasville, KY, USA) on growth and health of newly-weaned pigs under dietary challenge of multiple mycotoxins. Forty-eight newly-weaned pigs (21 d old) were individually allotted to four dietary treatments, based on a three phase-feeding, in a randomized complete block design (sex; initial BW) with two factors for 36 d. Two factors were dietary mycotoxins (deoxynivalenol: 2000 μg/kg supplemented in three phases; and aflatoxin: 200 μg/kg supplemented only in phase 3) and PYCW (0.2%). Growth performance (weekly), blood serum (d 34), and jejunal mucosa immune and oxidative stress markers (d 36) data were analyzed using MIXED procedure of SAS. Mycotoxins reduced (p < 0.05) average daily feed intake (ADFI) and average daily gain (ADG) during the entire period whereas PYCW did not affect growth performance. Mycotoxins reduced (p < 0.05) serum protein, albumin, creatinine, and alanine aminotransferase whereas PYCW decreased (p < 0.05) serum creatine phosphokinase. Neither mycotoxins nor PYCW affected pro-inflammatory cytokines and oxidative damage markers in the jejunal mucosa. No interaction was observed indicating that PYCW improved hepatic enzymes regardless of mycotoxin challenge. In conclusion, deoxynivalenol (2000 μg/kg, for 7 to 25 kg body weight) and aflatoxin B1 (200 μg/kg, for 16 to 25 kg body weight) impaired growth performance and nutrient digestibility of newly-weaned pigs, whereas PYCW could partially improve health of pigs regardless of mycotoxin challenge.

scholarly journals PSIV-B-42 Late-Breaking: Effects of dietary inactivated probiotics on growth performance, nutrient digestibility, and immune responses of weaned pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 327-328
Author(s):  
Joowon Kang ◽  
Jong Pyo Chae ◽  
S-H Kim ◽  
J-W Kim ◽  
Sangwoo Park ◽  
...  
Keyword(s):  
Immune Responses ◽  
Growth Performance ◽  
Block Design ◽  
Lactobacillus Rhamnosus ◽  
Experimental Period ◽  
Nutrient Digestibility ◽  
Weaned Pigs ◽  
Biotechnology Research ◽  
Tnf Α ◽  
Dietary Treatments

Abstract The study was conducted to investigate effects of dietary inactivated probiotics on growth performance, nutrient digestibility, and immune responses of weaned pigs. A total of 96 weaned pigs (initial BW = 6.95 ± 0.25 kg) were randomly assigned to 4 dietary treatments with increasing levels of inactive probiotics (4 pigs/replicate; 6 replicates/treatment; 0, 0.1, 0.2, and 0.4%; CON, T1, T2, and T3) in a randomized complete block design (BW and sex as blocks). The inactivated probiotics used in this experiment was a commercial product (CJ CheilJedang Biotechnology Research Institute, Seoul, Korea) containing 1 x 106 CFU/g on stains of Lactobacillus rhamnosus. Pigs were fed respective dietary treatments for 4 weeks. During the last week of the experimental period, pigs were fed their respective dietary treatments containing 0.2% chromic oxide. Fecal samples were collected by rectal palpation daily for the last 3 days after the 4-day adjustment period during the last week of experiment. Blood was collected from randomly selected one pig each pen on d 1, 3, 7, and 14 after weaning. Measurements were growth performance, apparent total tract digestibility (ATTD), TNF-α, TGF-β, CRP, and cortisol by ELISA. Data were analyzed using the MIXED procedure of SAS. The inactivated probiotics increased ADG (471, 501, and 513 vs. 428 g/d; P &lt; 0.05) and G:F (0.65, 0.69, and 0.71 vs. 0.58 g/g; P &lt; 0.05) during overall experimental period compared with CON. The inactivated probiotics increased ATTD of GE (86.87, 87.29, and 88.53 vs. 85.25%; P &lt; 0.05) compared with CON. The inactivated probiotics decreased TNF-α (600, 542, and 523 vs. 849 pg/ml; P &lt; 0.05) and cortisol (5.58, 5.56, and 5.44 vs. 7.25 ng/ml; P &lt; 0.05) on d 7 compared with CON. In conclusion, addition of inactivated probiotics improved growth performance, and nutrient digestibility, and modified immune responses.

scholarly journals PSVI-24 Growth performance and immune responses of weaned pigs from lactating sows fed dietary spray dried plasma

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 213-214
Author(s):  
Sheena Kim ◽  
Byeonghyeon Kim ◽  
Junsu Kim ◽  
Jeongjae Lee ◽  
Kwangwook Kim ◽  
...  
Keyword(s):  
Immune Responses ◽  
Growth Performance ◽  
Transforming Growth Factor ◽  
Experimental Period ◽  
Enzyme Linked Immunosorbent Assay ◽  
Weaned Pigs ◽  
Tnf Α ◽  
Lactating Sows ◽  
Dietary Treatments ◽  
Spray Dried

Abstract The experiment was conducted to investigate growth performance and immune responses of weaned pigs from lactating sows fed dietary spray dried plasma (SDP). A total of 12 sows (227.78 ± 7.50 kg BW; parity = 2.0 ± 0.7) were randomly assigned to 2 dietary treatments in a completely randomized design. Dietary treatments were a typical lactation diet based on corn-soybean meal (CON) and CON supplemented with 1% of SDP. Sows were fed the dietary treatments from d 30 before expected farrowing to weaning. Weaned pigs from each sow were transferred to a nursery barn and group-housed by dietary treatments of sows and fed a typical nursery diet for 6 wk. Blood samples were collected from randomly selected 2 weaned pigs from each litter on day of weaning day, d 3, and 7 postweaning. Serum tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), C-reactive protein (CRP), and cortisol were analyzed by the enzyme-linked immunosorbent assay. Body weights and feed allowance were recorded throughout post-weaning period to calculate growth performance of weaned pigs. Data were analyzed using the PROC GLM of SAS. Weaned pigs from lactating sows fed SDP tended (P < 0.10) to have increase ADG (470.47 vs. 414.52 g/d) during overall experimental period than pigs from lactating sows fed CON. Supplementation of SDP tended (P < 0.10) to decrease serum TNF-α on d 3 (344.11 vs. 449.80 pg/ml) and CRP on d 7 (78.41 vs. 112.28 ng/ml) compared with the piglets from lactating sows fed CON. The addition of SDP also reduced (P < 0.05) serum cortisol on d 3 (1.40 vs. 1.88 ng/ml) and TGF-β on d 7 (718.33 vs. 836.48 pg/ml) compared with CON. In conclusion, addition of dietary spray dried plasma in lactation diets may enhance growth performance and modulate immune responses of weaned pigs.

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