scholarly journals Rice aleurone layer specific OsNF-YB1 regulates grain filling and endosperm development by interacting with an ERF transcription factor

2016 ◽  
Vol 67 (22) ◽  
pp. 6399-6411 ◽  
Author(s):  
Jing-Jing Xu ◽  
Xiao-Fan Zhang ◽  
Hong-Wei Xue
Keyword(s):  
Transcription Factor ◽  
Grain Filling ◽  
Endosperm Development ◽  
Aleurone Layer ◽  
Erf Transcription Factor

scholarly journals CRISPR-Cas9 mediated mutation in GRAIN WIDTH and WEIGHT2 (GW2) locus improves aleurone layer and grain nutritional quality in rice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
V. Mohan Murali Achary ◽  
Malireddy K. Reddy
Keyword(s):  
Nutritional Quality ◽  
Crop Improvement ◽  
Grain Filling ◽  
Crop Productivity ◽  
Endosperm Development ◽  
Aleurone Layer ◽  
Rice Grain ◽  
Loss Of Function ◽  
Improvement Strategy ◽  
Grain Width

AbstractEnhancing crop productivity and their nutritional quality are the key components and primary focus of crop improvement strategy for fulfilling future food demand and improving human health. Grain filling and endosperm development are the key determinants of grain yield and nutritional quality. GRAIN WIDTH and WEIGHT2 (GW2) gene encodes a RING-type E3 ubiquitin ligase and determines the grain weight in cereal crops. Here we report GW2 knockout (KO) mutants in Indica (var. MTU1010) through CRISPR/Cas9 genome editing. The endosperm of GW2-KO mutant seed displays a thick aleurone layer with enhanced grain protein content. Further the loss of function of OsGW2 results in improved accumulation of essential dietary minerals (Fe, Zn, K, P, Ca) in the endosperm of rice grain. Additionally, the mutants displayed an early growth vigour phenotype with an improved root and shoot architecture. The hull morphology of GW2-KO lines also showed improved, grain filling thereby promoting larger grain architecture. Together, our findings indicate that GW2 may serve as a key regulator of improved grain architecture, grain nutritional quality and an important modulator of plant morphology. The study offers a strategy for the development of improved rice cultivars with enriched nutritional quality and its possible implementation in other cereals as well.

scholarly journals Genome Wide Analysis of the Transcriptional Profiles in Different Regions of the Developing Rice Grains

Rice ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Ting-Ying Wu ◽  
Marlen Müller ◽  
Wilhelm Gruissem ◽  
Navreet K. Bhullar
Keyword(s):  
Gene Expression ◽  
Dna Sequence ◽  
Expression Patterns ◽  
Grain Filling ◽  
Aleurone Layer ◽  
Rice Grain ◽  
Sequence Motifs ◽  
Grain Development ◽  
Spatio Temporal ◽  
Dna Sequence Motifs

Abstract Background Rice is an important food source for humans worldwide. Because of its nutritional and agricultural significance, a number of studies addressed various aspects of rice grain development and grain filling. Nevertheless, the molecular processes underlying grain filling and development, and in particular the contributions of different grain tissues to these processes, are not understood. Main Text Using RNA-sequencing, we profiled gene expression activity in grain tissues comprised of cross cells (CC), the nucellar epidermis (NE), ovular vascular trace (OVT), endosperm (EN) and the aleurone layer (AL). These tissues were dissected using laser capture microdissection (LCM) at three distinct grain development stages. The mRNA expression datasets offer comprehensive and new insights into the gene expression patterns in different rice grain tissues and their contributions to grain development. Comparative analysis of the different tissues revealed their similar and/or unique functions, as well as the spatio-temporal regulation of common and tissue-specific genes. The expression patterns of genes encoding hormones and transporters indicate an important role of the OVT tissue in metabolite transport during grain development. Gene co-expression network prediction on OVT-specific genes identified several distinct and common development-specific transcription factors. Further analysis of enriched DNA sequence motifs proximal to OVT-specific genes revealed known and novel DNA sequence motifs relevant to rice grain development. Conclusion Together, the dataset of gene expression in rice grain tissues is a novel and useful resource for further work to dissect the molecular and metabolic processes during rice grain development.

The AP2/ERF transcription factor SmERF1L1 regulates the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza

2019 ◽  
Vol 274 ◽  
pp. 368-375 ◽  
Author(s):  
Qiang Huang ◽  
Meihong Sun ◽  
Tingpan Yuan ◽  
Yu Wang ◽  
Min Shi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Phenolic Acids ◽  
Salvia Miltiorrhiza ◽  
Erf Transcription Factor

scholarly journals Mechanism of delayed seed germination caused by high temperature during grain filling in rice (Oryza sativa L.)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chetphilin Suriyasak ◽  
Yui Oyama ◽  
Toshiaki Ishida ◽  
Kiyoshi Mashiguchi ◽  
Shinjiro Yamaguchi ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
High Temperature ◽  
Seed Germination ◽  
Oryza Sativa L ◽  
Grain Filling ◽  
Aleurone Layer ◽  
Gene Promoters ◽  
Aba Catabolism ◽  
Amylase Genes ◽  
Transcriptional Changes

Abstract High temperature during grain filling considerably reduces yield and quality in rice (Oryza sativa L.); however, how high temperature affects seed germination of the next generation is not yet well understood. Here, we report that seeds from plants exposed to high temperature during the grain filling stage germinated significantly later than seeds from unstressed plants. This delay remained even after dormancy release treatments, suggesting that it was not due to primary seed dormancy determined during grain filling. In imbibed embryos of heat-stressed seeds, expression of abscisic acid (ABA) biosynthesis genes (OsNCEDs) was higher than in those of control seeds, whereas that of ABA catabolism genes (OsABA8′OHs) was lower. In the aleurone layer, despite no change in GA signaling as evidenced by no effect of heat stress on OsGAMYB gene expression, the transcripts of α-amylase genes OsAmy1C, OsAmy3B, and OsAmy3E were significantly down-regulated in heat-stressed seeds in comparison with controls. Changes in promoter methylation levels were consistent with transcriptional changes of ABA catabolism-related and α-amylase genes. These data suggest that high temperature during grain filling results in DNA methylation of ABA catabolism-related and α-amylase gene promoters, delaying germination of heat-stressed seeds.

scholarly journals Ethylene-Responsive AP2/ERF Transcription Factor MACD1 Participates in Phytotoxin-Triggered Programmed Cell Death

2013 ◽  
Vol 26 (8) ◽  
pp. 868-879 ◽  
Author(s):  
Keisuke Mase ◽  
Nobuaki Ishihama ◽  
Hitoshi Mori ◽  
Hideki Takahashi ◽  
Hironori Kaminaka ◽  
...  
Keyword(s):  
Cell Death ◽  
Transcription Factor ◽  
Programmed Cell Death ◽  
Fumonisin B1 ◽  
Structural Analog ◽  
Necrotrophic Pathogen ◽  
Factors Affecting ◽  
Downstream Signaling ◽  
Upregulated Genes ◽  
Erf Transcription Factor

To investigate plant programmed cell death (PCD), we developed the model system using phytotoxin AAL, which is produced by necrotrophic pathogen Alternaria alternata f. sp. lycopersici, and AAL-sensitive Nicotiana umbratica. We previously reported that ethylene (ET) signaling plays a pivotal role in AAL-triggered cell death (ACD). However, downstream signaling of ET to ACD remains unclear. Here, we show that the modulator of AAL cell death 1 (MACD1), which is an APETALA2/ET response factor (ERF) transcription factor, participates in ACD and acts downstream of ET signaling during ACD. MACD1 is a transcriptional activator and MACD1 overexpression plants showed earlier ACD induction than control plants, suggesting that MACD1 positively regulates factors affecting cell death. To investigate the role of MACD1 in PCD, we used Arabidopsis thaliana and a structural analog of AAL, fumonisin B1 (FB1). FB1-triggered cell death was compromised in ET signaling and erf102 mutants. The loh2 mutants showed sensitivity to AAL, and the loh2-1/erf102 double mutant compromised ACD, indicating that ERF102 also participates in ACD. To investigate the PCD-associated genes regulated by ERF102, we compared our microarray data using ERF102 overexpression plants with the database of upregulated genes by AAL treatment in loh2 mutants, and found genes under the control of ERF102 in ACD.

scholarly journals The Arabidopsis general transcription factor TFIIB1 (AtTFIIB1) is required for pollen tube growth and endosperm development

2013 ◽  
Vol 64 (8) ◽  
pp. 2205-2218 ◽  
Author(s):  
Jing-Jing Zhou ◽  
Yan Liang ◽  
Qian-Kun Niu ◽  
Li-Qun Chen ◽  
Xue-Qin Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Endosperm Development ◽  
Tube Growth ◽  
General Transcription Factor
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