The Stimulation of Acid Phosphatase Activity in the Stalked Gland of Drosera rotundifolia

ABSTRACT The acid phosphatase activity of the rat neurohypophysis was measured during increased gonadotrophic hormone levels, in diabetes insipidus (DI) (Brattleboro strain) and after water loading, i. e. conditions that interfere with the function of the hypothalamo-neurohypophyseal system (HNS). In addition determinations of tissue protein, lipid and DNA and of water metabolism were performed. Neurohypophyseal acid phosphatase activity expressed on a dry weight basis increased under all conditions. For gonadectomized females, Brattleboro rats and water loaded males an increased water metabolism was observed. The increased acid phosphatase activity is interpreted as being related to disposal of release residues during stimulation of the HNS (increased gonadotrophic hormone levels and DI) and to disposal of neurosecretory material during inhibition (water loading).

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Chloramphenicol stimulates acid phosphatase activity in germinating cotton (Gossypium hirsutum) embryos

Biochemical Journal ◽

10.1042/bj2120073 ◽

1983 ◽

Vol 212 (1) ◽

pp. 73-77 ◽

Cited By ~ 6

Author(s):

R Bhargava ◽

R C Sachar

Keyword(s):

Enzyme Activity ◽

Acid Phosphatase ◽

Gossypium Hirsutum ◽

Phosphatase Activity ◽

Acid Phosphatase Activity ◽

Molecular Form ◽

Significant Stimulation ◽

High Concentrations ◽

Fold Stimulation ◽

Stimulation Of

Low dosages of chloramphenicol (25-50 micrograms/ml) brought about a 2-4-fold stimulation of acid phosphatase activity in 48 h-germinated cotton (Gossypium hirsutum) embryos. However, at high concentrations of chloramphenicol (100-1000 micrograms/ml), there was a progressive decline in enzyme activity. The stimulatory effect of the drug on acid phosphatase activity was relatively specific, since no significant stimulation of activities of proteinase, deoxyribonuclease, ribonuclease, o-diphenolase and peroxidase was observed in germinating cotton embryos. Chloramphenicol, however, did promote the activities of isocitric lyase and alkaline phosphatase. Sephadex G-200 chromatography of the enzyme fraction revealed high (230 000)- and low (106 000)-molecular-weight multiple forms of acid phosphatase in the chloramphenicol-treated embryos, in contrast with a single molecular form (mol.wt. 106 000) in the untreated embryos. Thus the treatment of cotton embryos with chloramphenicol induced both a qualitative and a quantitative change in the acid phosphatase activity. Chloramphenicol-stimulated acid phosphatase activity was strongly inhibited when Pi was included in the germination medium. However, the control embryos showed less pronounced inhibition of enzyme activity in presence of Pi ions.

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Appearance of Hydrolase Rich Granules in Human Lymphocytes Induced by Phytohemagglutinin and Antigens

Blood ◽

10.1182/blood.v30.1.84.84 ◽

1967 ◽

Vol 30 (1) ◽

pp. 84-102 ◽

Cited By ~ 57

Author(s):

ROCHELLE HIRSCHHORN ◽

KURT HIRSCHHORN ◽

GERALD WEISSMANN

Keyword(s):

Acid Phosphatase ◽

Phosphatase Activity ◽

Acid Phosphatase Activity ◽

Human Peripheral Blood ◽

Human Lymphocytes ◽

Hydrolytic Enzyme ◽

Total Activity ◽

Peripheral Blood Lymphocytes ◽

Streptolysin S ◽

Stimulation Of

Abstract Purified human peripheral blood lymphocytes have been shown to develop acid hydrolase-rich granules between 24 and 48 hours after stimulation by phytohemagglutinin, prior to mitosis. This increase has been measured biochemically as a net increase in total activity of the lysosomal enzymes: acid β-glycerophosphatase, acid phenolpthalein phosphatase, and aryl sulfatase. The subcellular localization of acid hydrolytic enzyme activities has been investigated, and they have been shown to be concentrated in a large granule fraction of sucrose homogenates and to behave as if they were membrane-bounded, in that their activity could be released by lysolecithin. It has also been demonstrated by histochemical technics that stimulation of lymphocytes by antigen (PPD) and by streptolysin S, as well as by phytohemagglutinin, produced an increase in acid phosphatase activity. Chloroquine, an inhibitor of the response to phytohemagglutinin, has also been shown to inhibit the development of acid phosphatase activity. These results are interpreted to suggest that both specific and nonspecific stimulants of lymphocytes induce lysosome-like structures in premitotic cells.

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Erythrophagocytosis in the Liver in Hemolytic Anemias

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100100664 ◽

1968 ◽

Vol 26 ◽

pp. 184-185

Author(s):

O. T. Minick ◽

E. Orfei ◽

F. Volini ◽

G. Kent

Keyword(s):

Acid Phosphatase ◽

Oxidative Damage ◽

Phosphatase Activity ◽

Kupffer Cells ◽

Acid Phosphatase Activity ◽

Common Type ◽

Red Cell ◽

Cell Fragments ◽

Reticulo Endothelial System ◽

Important Site

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).

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