Biochemical Studies on Sulfate-reducing BacteriaXII. Some Properties of Flavodoxin from Desulfovibrio vulgaris

1973 ◽  
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Desulfovibrio Vulgaris ◽  
Sulfate Reducing ◽  
Biochemical Studies ◽  
Reducing Bacteria

BIOCHEMICAL STUDIES ON SULFATE-REDUCING BACTERIA

1957 ◽  
Vol 44 (7) ◽  
pp. 413-423 ◽  
Author(s):  
MAKOTO ISHIMOTO ◽  
JIRO KOYAMA ◽  
TATSUHIKO YAGI ◽  
MASARU SHIRAKI
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Biochemical Studies ◽  
Reducing Bacteria

BIOCHEMICAL STUDIES ON SULFATE-REDUCING BACTERIA

1954 ◽  
Vol 41 (5) ◽  
pp. 537-546 ◽  
Author(s):  
MAKOTO ISHIMOTO ◽  
JIRO KOYAMA ◽  
TSUNEO OMURA
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Biochemical Studies ◽  
Reducing Bacteria

scholarly journals Microbial Corrosion of API 5L X-70 Carbon Steel by ATCC 7757 and Consortium of Sulfate-Reducing Bacteria

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Arman Abdullah ◽  
Nordin Yahaya ◽  
Norhazilan Md Noor ◽  
Rosilawati Mohd Rasol
Keyword(s):  
Weight Loss ◽  
Carbon Steel ◽  
Corrosion Rate ◽  
Sulfate Reducing Bacteria ◽  
Desulfovibrio Vulgaris ◽  
Corrosion Morphology ◽  
Sulfate Reducing ◽  
Weight Loss Method ◽  
Loss Method ◽  
Reducing Bacteria

Various cases of accidents involving microbiology influenced corrosion (MIC) were reported by the oil and gas industry. Sulfate reducing bacteria (SRB) have always been linked to MIC mechanisms as one of the major causes of localized corrosion problems. In this study, SRB colonies were isolated from the soil in suspected areas near the natural gas transmission pipeline in Malaysia. The effects of ATCC 7757 and consortium of isolated SRB upon corrosion on API 5L X-70 carbon steel coupon were investigated using a weight loss method, an open circuit potential method (OCP), and a potentiodynamic polarization curves method in anaerobic conditions. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were then used to determine the corrosion morphology in verifying the SRB activity and corrosion products formation. Results from the study show that the corrosion rate (CR) of weight loss method for the isolated SRB is recorded as 0.2017 mm/yr compared to 0.2530 mm/yr for ATCC 7757. The Tafel plot recorded the corrosion rate of 0.3290 mm/yr for Sg. Ular SRB and 0.2500 mm/yr forDesulfovibrio vulgaris. The results showed that the consortia of isolated SRB were of comparable effects and features with the single ATCC 7757 strain.

Biochemical Studies on Sulfate-reducing Bacteria

1974 ◽  
Vol 75 (3) ◽  
pp. 519-529 ◽  
Author(s):  
Kunihiko KOBAYASHI ◽  
Yasuhide SEKI ◽  
Makoto ISHIMOTO
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Biochemical Studies ◽  
Reducing Bacteria

Biochemical Studies on Sulfate-Reducing Bacteria

1975 ◽  
Vol 78 (5) ◽  
pp. 1079-1085 ◽  
Author(s):  
Kunihiko KOBAYASHI ◽  
Yuko MORISAWA ◽  
Taiko ISHITUKA ◽  
Makoto ISHIMOTO
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Biochemical Studies ◽  
Reducing Bacteria

BIOCHEMICAL STUDIES ON SULFATE-REDUCING BACTERIA

1955 ◽  
Vol 42 (1) ◽  
pp. 41-53 ◽  
Author(s):  
MAKOTO ISHIMOTO ◽  
JIRO KOYAMA ◽  
YUTAKA NAGAI
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Biochemical Studies ◽  
Reducing Bacteria

scholarly journals Polyhydroxyalkanoate (PHA) Accumulation in Sulfate-Reducing Bacteria and Identification of a Class III PHA Synthase (PhaEC) in Desulfococcus multivorans

2004 ◽  
Vol 70 (8) ◽  
pp. 4440-4448 ◽  
Author(s):  
Tran Hai ◽  
Daniela Lange ◽  
Ralf Rabus ◽  
Alexander Steinbüchel
Keyword(s):  
Amino Acid ◽  
Dry Matter ◽  
Sulfate Reducing Bacteria ◽  
Pha Synthase ◽  
Desulfovibrio Vulgaris ◽  
Class Iii ◽  
Sulfate Reducing ◽  
Desulfobacterium Autotrophicum ◽  
Reducing Bacteria ◽  
Desulfococcus Multivorans

ABSTRACT Seven strains of sulfate-reducing bacteria (SRB) were tested for the accumulation of polyhydroxyalkanoates (PHAs). During growth with benzoate Desulfonema magnum accumulated large amounts of poly(3-hydroxybutyrate) [poly(3HB)]. Desulfosarcina variabilis (during growth with benzoate), Desulfobotulus sapovorans (during growth with caproate), and Desulfobacterium autotrophicum (during growth with caproate) accumulated poly(3HB) that accounted for 20 to 43% of cell dry matter. Desulfobotulus sapovorans and Desulfobacterium autotrophicum also synthesized copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyvalerate when valerate was used as the growth substrate. Desulfovibrio vulgaris and Desulfotalea psychrophila were the only SRB tested in which PHAs were not detected. When total DNA isolated from Desulfococcus multivorans and specific primers deduced from highly conserved regions of known PHA synthases (PhaC) were used, a PCR product homologous to the central region of class III PHA synthases was obtained. The complete pha locus of Desulfococcus multivorans was subsequently obtained by inverse PCR, and it contained adjacent phaEDm and phaCDm genes. PhaC Dm and PhaE Dm were composed of 371 and 306 amino acid residues and showed up to 49 or 23% amino acid identity to the corresponding subunits of other class III PHA synthases. Constructs of phaCDm alone (pBBRMCS-2::phaCDm ) and of phaEDmCDm (pBBRMCS-2::phaEDmCDm ) in various vectors were obtained and transferred to several strains of Escherichia coli, as well as to the PHA-negative mutants PHB−4 and GPp104 of Ralstonia eutropha and Pseudomonas putida, respectively. In cells of the recombinant strains harboring phaEDmCDm small but significant amounts (up to 1.7% of cell dry matter) of poly(3HB) and of PHA synthase activity (up to 1.5 U/mg protein) were detected. This indicated that the cloned genes encode functionally active proteins. Hybrid synthases consisting of PhaC Dm and PhaE of Thiococcus pfennigii or Synechocystis sp. strain PCC 6308 were also constructed and were shown to be functionally active.

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