scholarly journals Complete Genome Sequence Data of Nonpathogenic Strain Rhizobium vitis VAR03-1, a Biological Control Agent for Grapevine Crown Gall Disease

2020 ◽  
Vol 33 (12) ◽  
pp. 1451-1453
Author(s):  
Yoshiteru Noutoshi ◽  
Atsushi Toyoda ◽  
Tomoya Ishii ◽  
Kirara Saito ◽  
Megumi Watanabe ◽  
...  
Keyword(s):  
Biological Control ◽  
Genome Sequence ◽  
Crown Gall ◽  
Complete Genome ◽  
Sequence Data ◽  
Biological Control Agent ◽  
Suppressive Effect ◽  
Control Agent ◽  
Crown Gall Disease ◽  
Nonpathogenic Strain

Crown gall disease in grapevine is caused by pathogenic strains of Rhizobium vitis with a tumor-inducing (Ti) plasmids. A nonpathogenic strain, VAR03-1 of R. vitis, has been isolated from the grapevine root of nursery stock and it was shown to act as a biological control agent to crown gall disease. Its disease-suppressive effect was observed even when it was coinoculated with the pathogen in a 1:1 ratio. Here, we present the complete genome data of R. vitis VAR03-1, assembled by sequencing reads obtained by both PacBio and Illumina technologies with annotation. This genome sequence could contribute to investigations of the molecular basis underlying the biocontrol activity as well as the root-colonization ability of this bacterial strain. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

scholarly journals Complete Genome Sequence Data of the Grapevine Crown Gall inhibiting bacteria Allorhizobium vitis Strain F2/5.

2021 ◽  
Author(s):  
Hangwei Xi ◽  
Joshua Grist ◽  
Maarten Ryder ◽  
Iain Searle
Keyword(s):  
Genome Sequence ◽  
Crown Gall ◽  
Sequence Data ◽  
Biological Control Agent ◽  
Control Agent ◽  
Protein Coding ◽  
Crown Gall Disease ◽  
Allorhizobium Vitis ◽  
Grapevine Crown Gall ◽  
Circular Chromosomes

Crown gall disease in grapevine is caused by pathogenic strains of Allorhizobium vitis. A. vitis strain F2/5 is a non-pathogenic biocontrol agent that was previously shown to act as a biological control agent to crown gall disease and first isolated from South Africa. Here, we present the complete assembled genome and is 5.94 Mb in length with 5,414 predicted protein-coding sequences, has two circular chromosomes and five plasmids. The genome sequence has no detectable T-DNA border sequences and is missing key virulence genes which is consistent with the bacteria being non-pathogenic. The F2/5 genome sequence could contribute to understanding the molecular basis underlying the biocontrol activity.

scholarly journals Biological Control of Crown Gall of Grapevine, Rose, and Tomato by Nonpathogenic Agrobacterium vitis Strain VAR03-1

2008 ◽  
Vol 98 (11) ◽  
pp. 1218-1225 ◽  
Author(s):  
A. Kawaguchi ◽  
K. Inoue ◽  
Y. Ichinose
Keyword(s):  
Biological Control ◽  
Crown Gall ◽  
Biological Control Agent ◽  
Inhibitory Effect ◽  
Control Agent ◽  
Inhibitory Effects ◽  
Agrobacterium Vitis ◽  
Colony Forming Units ◽  
Nonpathogenic Strain ◽  
A Cell

A nonpathogenic strain of Agrobacterium vitis VAR03-1 was tested as a biological control agent for crown gall of grapevine (Vitis vinifera). When roots of grapevine, rose (Rose multiflora), and tomato (Lycopersicon esculentum) were soaked in a cell suspension of antagonists before planting in soil infested with tumorigenic A. vitis, A. rhizogenes, and A. tumefaciens, respectively, treatment with VAR03-1 significantly reduced the number of plants with tumors and disease severity in the three plant species. The inhibitory effects of treatment with VAR03-1 and the nonpathogenic A. rhizogenes strain K84 on crown gall of rose and tomato were almost identical, and the inhibitory effect of VAR03-1 on grapevine was superior to that of K84. Moreover, VAR03-1 greatly controlled crown gall of grapevine due to tumorigenic A. vitis in the field. VAR03-1 established populations averaging 106 colony forming units (CFU)/g of root in the rhizosphere of grapevine and persisted on roots for 2 years. VAR03-1 was bacteriocinogenic, producing a halo of inhibition against those three species of Agrobacterium. This is the first report that a nonpathogenic strain, VAR03-1, can effectively control crown gall caused by tumorigenic A. vitis, A. rhizogenes, and A. tumefaciens.

scholarly journals Complete Genome Sequence Data of Tumorigenic Rhizobium vitis Strain VAT03-9, a Causal Agent of Grapevine Crown Gall Disease

2020 ◽  
Vol 33 (11) ◽  
pp. 1280-1282
Author(s):  
Yoshiteru Noutoshi ◽  
Atsushi Toyoda ◽  
Tomoya Ishii ◽  
Kirara Saito ◽  
Megumi Watanabe ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Crown Gall ◽  
Complete Genome ◽  
Sequence Data ◽  
Causal Agent ◽  
Crown Gall Disease ◽  
Grapevine Crown Gall ◽  
Okayama Prefecture ◽  
Open Access Article

Rhizobium vitis strain VAT03-9 (MAFF 211676) is a causal agent of crown gall disease in grapevine. It is one of the pathogenic strains of R. vitis isolated from graft unions of grapevine in Okayama Prefecture, Japan. Inoculation tests verified its virulence for gall formation on grapevine, tomato, and sunflower. It harbors tumor-inducing plasmid. Here, we present the complete genome sequence with annotation of R. vitis VAR03-9 obtained by assembling reads from PacBio and Illumina-sequencers. This genome sequence should be useful for the analyses of pathogenicity and evolutionary lineage of the pathogens of crown gall disease. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

scholarly journals Complete Genome Sequence Data of rare Actinomycetes Strain, Saccharothrix texasensis strain 6-C, a Biological Control Agent for Potato Late Blight

2021 ◽  
Author(s):  
Shun Feng ◽  
Yongfei Jian ◽  
Liang Jin ◽  
Shicai Tang ◽  
Zhengguo Li
Keyword(s):  
Biological Control ◽  
Late Blight ◽  
Genome Sequence ◽  
Complete Genome ◽  
Biological Control Agent ◽  
Gc Content ◽  
Potato Late Blight ◽  
Control Agent ◽  
Type I ◽  
Rare Actinomycetes

A rare actinomycetes strain of Saccharothrix texasensis strain 6-C, has been isolated from the potato rhizosphere and it was shown to act as a biological control agent to potato late blight. It is also the first report on Saccharothrix inhibiting P. infestans. Here, we present the complete genome data of S. texasensis strain 6-C, assembled by sequencing reads obtained by both PacBio and Illumina technologies with annotation. The final assembled genome length is 9,045,220 bp, with no gaps, no plasmid, and its GC content is 72.39%. Nine non-ribosomal peptide synthetase (NRPS), five type I polyketide synthase (T1PKS), four terpene and three lanthipeptide gene clusters were identified in the genome, which would be likely to encode lots of antimicrobial active substances to help host plants against disease. This genome sequence could contribute to investigations of the molecular basis underlying the biocontrol activity of this Saccharothrix strain.

scholarly journals Characterization of Migration and Induced Resistance of Rhizobium Vitis Strain ARK-1, a Biological Control Agent Against Grapevine Crown Gall Disease

2020 ◽  
Author(s):  
Akira Kawaguchi ◽  
Yoshiteru Noutoshi
Keyword(s):  
Crown Gall ◽  
Temporal Dynamics ◽  
Biological Control Agent ◽  
Control Agent ◽  
Systemic Resistance ◽  
Marker Genes ◽  
Cell Detection ◽  
Induced Expression ◽  
Crown Gall Disease ◽  
Grapevine Crown Gall

Abstract A nonpathogenic strain ARK-1 of Rhizobium vitis has an antagonistic activity toward tumorigenic (Ti) strain of R. vitis, a causal agent of grapevine crown gall disease, and works as a biocontrol agent. We have demonstrated that the gall formation was fully suppressed when ARK-1 was co-inoculated with Ti into the grapevine stem at a 1:1 ratio. For practical use of ARK-1 in agriculture, understanding the temporal dynamics of the bacterial habitat on host plants and the biocontrol property are needed in order to develop proper application methods. Here we demonstrated that the gall incidence by Ti was reduced to about 50% when ARK-1 was pre-inoculated at both upper and lower positions on the grapevine stem 3 cm away from the Ti-inoculation point 5 days before. The bacterial cell detection assay in the grapevine tissue revealed that ARK-1 could migrate at least 3 cm in 5 days. Inoculations of ARK-1 or Ti induced expression of marker genes for defense-related phytohormones such as salicylic acid, jasmonic acid, and ethylene in grapevine within 3 days but they were diminished by 6 days. Inoculation of Ti 5 days after ARK-1 pre-inoculation induced expression of the marker genes except for the LOX-9 gene in a basically similar way to those without the pre-inoculation, suggesting that ARK-1 did not induce typical acquired systemic resistance or induced systemic resistance in grapevine, while the transcript of LOX-9 was detected at 24 and 48 hours after the Ti inoculation when ARK-1 was pre-inoculated, unlike the un-inoculated condition. ARK-1 primed the induction of certain defense genes and it may take part in its biocontrol activity.

scholarly journals Antagonistic potential of native agrocin-producing non-pathogenic Agrobacterium tumefaciens strain UHFBA-218 to control crown gall in peach

2017 ◽  
Vol 97 (1) ◽  
pp. 1-11
Author(s):  
Aditi Sharma ◽  
A. K. Gupta ◽  
K. Khosla ◽  
Rishi Mahajan ◽  
Bharti ◽  
...  
Keyword(s):  
Biological Control ◽  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Room Temperature ◽  
Biological Control Agent ◽  
Control Agent ◽  
Stone Fruits ◽  
Copper Oxychloride ◽  
Antagonistic Potential ◽  
Different Doses

A non-pathogenic agrocin-producing native isolate ofAgrobacterium tumefaciensstrain UHFBA-218 was tested as a biological control agent against the peach crown gall. This strain was compatible with all the recommended pesticides used in stone fruits in the integrated pest management (IPM) module, except for copper oxychloride, which was detrimental to its growth. Upon artificial co-inoculation of 4-wk-old plants of tomato var. Solan Gola withA. tumefaciensstrain UHFBA-218 and tumorigenicA. tumefaciensstrain Peach 2E-10, out of the 27 isolates recovered, six were transconjugants showing selective acquisition of tumorigenic factors as made evident by amplification withiptandvirD2primers, whereas the rest of the isolates did not acquire any of these tumorigenic factors. A white stone powder-based formulation of this isolate (103.3 × 108cfu g-1) retained appreciable viability for up to 6 months at room temperature. When peach roots and seeds were soaked in cell suspensions of different doses of a white stone powder-based bioformulation of UHFBA-218 before planting in the field, the number of plants with tumours was reduced, with the lowest incidence of crown gall being observed in the 0.1% UHFBA-218 root dip treatment, i.e. 1.48% and 0.80% during the years 2013 and 2014, respectively. No incidence of crown gall was observed in the three seed dip treatments, i.e. 30-min dip in UHFBA-218 followed by 1 h of shade drying, stratified seeds dipped for 30 min in 0.1% suspensions of strains UHFBA-218 or K84 followed by 1 h of shade drying before sowing, as compared with 14.76% incidence in untreated plants.

scholarly journals Draft Genome Sequence of the Mycoparasitic Oomycete Pythium periplocum Strain CBS 532.74

2017 ◽  
Vol 5 (12) ◽  
Author(s):  
Sandeep K. Kushwaha ◽  
Ramesh R. Vetukuri ◽  
Laura J. Grenville-Briggs
Keyword(s):  
Biological Control ◽  
Genome Sequence ◽  
Biological Control Agent ◽  
Draft Genome ◽  
Pathogenic Fungi ◽  
Control Agent ◽  
Draft Genome Sequence ◽  
Plant Pathogenic Fungi ◽  
Protein Coding ◽  
Protein Coding Genes

ABSTRACT The oomycete Pythium periplocum is an aggressive mycoparasite of a number of plant pathogenic fungi and oomycetes and therefore has potential as a biological control agent. Here, we report the first draft genome sequence of P. periplocum, which comprises 35.89 Mb. It contains 1,043 scaffolds and 14,399 predicted protein-coding genes.

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