Effect ofTrichoderma harzianumSeed Treatment andRhizoctonia solaniInoculum Concentration on Damping-Off of Snap Bean in Acidic Soils

Emergence of snap beans (Phaseolus vulgaris L.) in field soil in 1995 to 1997 was reduced by the addition of dried, ground canola [Brassica napus L. ssp. oleifera (Metzg.) Sinsk. f. biennis] leaves and petioles to the furrow at planting. Soil amendment with the tissue increased the number of nodules on bean roots in all years. In plots with reduced stand, leaf area was increased and yield on a per-plant basis was larger than in plots with a better stand. Total yield was increased in plots with fewer plants only in 1995. Frequency of isolation of fungi that cause damping-off was not affected by the addition of canola at planting. When used as a seed treatment and incorporated at planting, canola residues were detrimental to emergence of snap bean.

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Enhancement of Snap Bean Emergence by Gliocladium virens

HortScience ◽

10.21273/hortsci.31.6.984 ◽

1996 ◽

Vol 31 (6) ◽

pp. 984-985 ◽

Cited By ~ 2

Author(s):

V.L. Smith

Keyword(s):

Biocontrol Agent ◽

Total Yield ◽

Field Soil ◽

Damping Off ◽

Snap Bean ◽

Ambient Temperatures ◽

Snap Beans ◽

Gliocladium Virens ◽

Pellet Formulation ◽

Plant Basis

Emergence of snap beans (Phaseolus vulgaris L.) in field soil in 1993–95 was enhanced by the biocontrol agent Gliocladium virens J.H. Miller, J.E. Giddens, & A.A. Foster. The fungus was applied to each seed at planting as a wheat bran alginate pellet formulation in 1993–95. Preemergence and postemergence damping-off were reduced in plots treated with G. virens. Nodulation on the roots of treated plants was numerically increased in 1993 and 1994 compared to nontreated plots. Efficacy of G. virens was reduced in 1995, probably due to high ambient temperatures at the time of planting. In plots with reduced stand, leaf area was increased and yield on a per-plant basis was larger than in plots with a better stand. Total yield also was increased in plots with fewer plants, except in 1994. Fungi isolated from failed seedlings included Fusarium spp., Pythium spp., and Rhizoctonia solani Kühn.

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Sterile White Basidiomycete Fungus Marasmius graminum: A New Pathogen Causing Seedling Blight in Rice

Plant Disease ◽

10.1094/pdis-05-20-1136-pdn ◽

2020 ◽

Author(s):

Shankar Prasad Gaire ◽

Xin-Gen (Shane) Zhou ◽

Young-Ki Jo

Keyword(s):

Morphological Characteristics ◽

Its Region ◽

Crown Rot ◽

Stem Rot ◽

Growth Chamber ◽

Its Sequencing ◽

Damping Off ◽

Seedling Blight ◽

Snap Bean ◽

Basidiomycete Fungus

In April 2018, damping-off of rice (Oryza sativa L.) seedlings at the 2-to-3-leaf stage was observed in three fields in the counties of Wharton and Matagorda of Texas and Jefferson-Davis Parish of Louisiana. All affected areas were 1 ha or greater, with 10 to 20% of the seedlings showing the symptoms. Infected seedlings showed dark-brown necrotic lesions on the roots and/or mesocotyls where white superficial mycelium was usually present. Symptomatic tissues excised from 10 diseased seedlings of each field were surface sterilized with 1% NaOCl, double rinsed in sterilized distilled water, and plated on potato dextrose agar (PDA). The plates were incubated at 25°C with a 12-h photoperiod in a growth chamber. After 48 h, hyphal tips of fungal colonies were transferred onto PDA and 12 isolates were obtained. Clamp connections and dolipore septa were observed in young hyphae, indicating that these isolates were a basidiomycete fungus. Young hyphal cells were binucleate based on safranin O stain (Bandoni 1979). No fruiting bodies or sclerotia produced on PDA after one month of incubation. Based on these morphological characteristics, these isolates were identified as belonging to sterile white basidiomycetes (SWB) (Howard et al. 1977). To further identify the isolates into the species level, the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) of a representative isolate was sequenced with primer ITS1 and ITS4 (Vinnere et al. 2005). The ITS sequence (GenBank acc. no. MT524457) had more than 97% sequence similarity with known Marasmius graminum strains from Denmark (JN943595) (Schoch et al. 2012) and Sweden (MH857692) (Vu et al. 2019). Pathogenicity was tested with three representative isolates in a growth chamber using a modified method (Carling and Leiner 1990). Pots (6.5 cm in diameter x 7.5 cm height) were filled with 100 g of sterilized sand and watered to field capacity. Five PDA plugs (4 mm in diameter) from 5-day-old growing culture were placed on the sand surface of each pot. Pots inoculated with PDA plugs without fungus served as the controls. Five seeds of rice cv. Presidio were planted into each pot and covered with 10 g of sterilized sand. Pots were maintained at 25±2°C in a growth chamber with a 12-h photoperiod for 14 days. There were four replicated pots for each treatment and the experiment repeated twice. After 2 weeks, severe damping-off and associated symptoms similar to those observed in the field appeared in the inoculated pots. No symptoms developed in the control pots. The same fungus was consistently re-isolated from infected plants. Based on morphological characteristics and rDNA-ITS sequencing, these isolates were identified as M. graminum. The SWB fungus was first reported as a causal agent of stem rot of snap bean in Florida (Howard et al. 1977) and Nebraska (Harveson 2002), root or hypocotyl rot of corn, snap bean, squash and peanut in Georgia (Sumner et al. 1979; Bell and Sumner 1984), and crown rot of pigeon pea (Cajanus cajan) in Puerto Rico (Kaiser et al. 1987). Later, the SWB strain (ATCC 28344) causing stem rot of snap bean in Florida was further identified as M. graminum based on nuclear large subunit rRNA gene (Vinnere et al. 2005). Comparing the ITS region of this isolate (AY445120) with our isolate revealed a 99% similarity. To our knowledge, this is the first report that the SWB fungus M. graminum causes seedling blight in rice. Identification of this new disease will help to develop management strategies for control of stand loss in rice.

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Identification and Characterization of Isolates of Pythium and Phytophthora spp. from Snap Beans with Cottony Leak

Plant Disease ◽

10.1094/pdis-06-15-0662-re ◽

2016 ◽

Vol 100 (7) ◽

pp. 1446-1453 ◽

Cited By ~ 5

Author(s):

J. D. Olson ◽

J. P. Damicone ◽

B. A. Kahn

Keyword(s):

Morphological Characteristics ◽

Pythium Ultimum ◽

The United States ◽

Phytophthora Nicotianae ◽

Damping Off ◽

Internal Transcribed Spacer Region ◽

Snap Bean ◽

Snap Beans ◽

Phytophthora Spp ◽

Phytophthora Drechsleri

Cottony leak is an important disease of snap bean in Oklahoma and nearby states. Oomycete pathogens isolated from diseased pods collected from commercial fields and research plots consisted of both Pythium spp. (n = 131) and Phytophthora spp. (n = 46). Isolates were identified to species by morphological characteristics and by sequencing a portion of the internal transcribed spacer region of representative isolates. The most common Pythium spp. were Pythium ultimum var. ultimum; Pythium ‘group HS’, a self-sterile form of P. ultimum that produces hyphal swellings in lieu of sporangia (n = 74); and P. aphanidermatum (n = 50). Phytophthora spp. included Phytophthora drechsleri (n = 41) and P. nicotianae (n = 5). Nearly all of the isolates (95%) and all species were pathogenic on detached pods but Pythium ultimum var. ultimum and Pythium ‘group HS’ were most aggressive. Phytophthora drechsleri was most aggressive on seedlings, causing preemergence damping off and seed rot. Pythium ultimum var. ultimum, Pythium ‘group HS’, and P. aphanidermatum were intermediate in virulence to seedlings, causing root rot, stunting, and limited postemergence damping off. Phytophthora nicotianae and Pythium diclinum (n = 4) were not pathogenic on seedlings. Most (87%) isolates were sensitive to metalaxyl-M (concentration that caused a 50% reduction in mycelial growth [EC50] < 1 μg/ml) and the rest were intermediate in sensitivity (EC50 > 1 to < 100 μg/ml). Phytophthora drechsleri was the most sensitive species (EC50 = 0.06 μg/ml) compared with Pythium aphanidermatum, which was least sensitive (EC50 = 1.3 μg/ml). Cottony leak is a disease complex caused by several oomycete species that should include Phytophthora drechsleri, a newly reported pathogen of snap bean in the United States.

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The effectiveness of granular chalk use for growing winter wheat on grey forest soil

Agrobìologìâ ◽

10.33245/2310-9270-2020-157-1-181-191 ◽

2020 ◽

pp. 181-191

Author(s):

M. Tkachenko ◽

N. Borys ◽

Ye. Kovalenko

Keyword(s):

Winter Wheat ◽

Physicochemical Properties ◽

Forest Soil ◽

Active Substance ◽

Mass Fraction ◽

Gray Forest Soil ◽

Mineral Fertilizers ◽

Acidic Soils ◽

Grey Forest Soil

The research aims to establish the eff ectiveness of granular chalk use produced by «Slavuta-Calcium» Ltd. under growing Poliska–90 winter wheat variety, changing the physicochemical properties of grey forest soil and the wheat productivity. It also aims to establish optimal dosis of «Slavuta-Calcium» granular chalk as the meliorant and mineral fertilizer for grey forest soil in the system of winter wheat fertilization. In the temporary fi eld studies, various doses of nutrients N60–90–120P30–45–60K60–90–120 combined with «Slavuta–Calcium» granular chalk in a dose of Ca230–460–690 kg/ha of the active substance were studied against the background of secondary plowing of rotation products – soybean biomass that averaged 2.34 t/ha. Granular chalk is a modern complex highly eff ective meliorant with the content of Ca – 37.7 and Mg – 0.2 %, the mass fraction of carbonates (CaCO3 + MgCO3) makes at least 95 %. It is characterized by a high level of solubility when interacting with moisture in soil. It has a form of white granules, the mass fraction of 4.0–6.0 mm in size granules makes not less than 90 % and the one of 1.0 mm in size makes less than 5 %. Reactivity – 97 %. The granular chalk is advisable to apply on acidic soils, as a highly concentrated calcium-magnesium fertilizer, with the former as the dominant fertilizer, to optimize the physicochemical properties of the soil, as well as the plant nutrition system, in particular, increasing the availability of an element for assimilation by plants and as long-term ameliorants. The eff ectiveness of the use of mineral fertilizers, in particular acidic nitrogen on highly and medium acidic soils, after chemical reclamation is increased by 30–50 %, and slightly acidic by 15–20 %. The increase in productivity of crops from the combined eff ects of nutrients and chalk granulated is usually higher than when separately applied. The eff ectiveness of the integrated action of these elements is manifested in the growth of plant productivity and the quality of the resulting products, as well as the optimization of physical chemical properties and soil buff ering in the long term. In order to optimize the physicochemical properties of the arable layer of gray forest soil and the productive nutrition of agricultural crops, winter wheat, in particular, biogenic elements should be used in doses N60-90-120P30-45- 60K60-90-120 with granulated chalk «Slavuta-Calcium» in doses of Ca230-460-690 kg/ha of active substance. Granulated chalk obtained as a result of industrial grinding of solid sedimentary carbonate rocks of natural origin, subsequently under the infl uence of the granulation process of the starting material contains Ca and Mg carbonates of at least 95 %, dense granules which facilitates convenient mechanized application, as well as chalk suitable for accurate metered application on the quest map. Key words: granular chalk, gray forest soil, chemical reclamation, crop productivity.

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Damping-off of sugar beet in Finland: II. Disease control

Agricultural and Food Science ◽

10.23986/afsci.72140 ◽

1983 ◽

Vol 55 (5) ◽

pp. 431-450

Author(s):

Mauritz Vestberg ◽

Risto Tahvonen ◽

Kyösti Raininko

Keyword(s):

Sugar Beet ◽

Disease Control ◽

Field Experiments ◽

Damping Off ◽

Good Protection ◽

Great Damage

In pot and field experiments carried out in 1979-1981, the systemic funqicide hymexazol prevented satisfactorily soil borne damping-off of sugar beet caused mainly by the fungus Pythium debaryanum auct. non Hesse. The results with the combination hymexazol + thiram were still better. This treatment gave very good protection against the disease up to about two to three weeks after emergence, increased the yield on the average by 5-10 % and produced considerably thicker and denser stands. Thereafter a large number of beets may have become infected, but no great damage was caused as only few died. Band spraying at emergence using hymexazol with a large amount of water as well as spraying into the seed furrow prevented the outbreak of the disease almost completely. Liming had little effect on damping-off.

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