Maize Seed Contamination and Seed Transmission of Maize Chlorotic Mottle Virus in Kenya
Maize chlorotic mottle virus (MCMV) causes maize lethal necrosis disease in combination with a cereal infecting potyvirus, leading to high yield losses. There is limited information on seed infection or contamination rate by MCMV and its comparison to transmission rate to maize seedlings. This study was conducted to determine the extent of seed contamination in seed lots from MCMV-infected maize fields in Kenya and the transmission of MCMV from seeds to seedlings. To determine the contamination levels, whole seeds were ground, and the extract tested for the presence of MCMV using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Seedling grow-outs were tested for seed transmission of MCMV using DAS-ELISA and real-time reverse transcription polymerase chain reaction (real-time RT-PCR) methods. The seed contamination rates of the four seed lots tested ranged from 4.9 to 15.9%. MCMV transmission frequency for 37,617 seedlings, tested in 820 pools of varying seed amounts by DAS-ELISA, was 0.17%, while a transmission frequency of 0.025% was obtained from 8,322 seedlings tested in 242 pools by real-time RT-PCR. Seeds from plants mechanically inoculated with MCMV had an overall seed transmission rate of 0.04% in 7,846 seedlings tested in 197 pools. The study showed that even with substantial contamination of maize seed with MCMV, the transmission of the virus from the seed to seedlings was low. Nevertheless, even low rates of transmission can be significant under field conditions where insect vectors can further spread the disease from infected seedlings, unless diseased plants are detected in time and properly managed.