Hepatitis A virus and polymerase chain reaction amplification

1995 ◽  
Vol 6 (Sup 2) ◽  
pp. S27-S31 ◽  
Author(s):  
B. H. Robertson ◽  
A. Normann ◽  
J. Graff ◽  
B. Flehmig ◽  
D. Friedberg ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Polymerase Chain Reaction Amplification ◽  
Chain Reaction ◽  
Reaction Amplification ◽  
Polymerase Chain

Polymerase chain reaction amplification and typing of rotavirus in environmental samples

1995 ◽  
Vol 31 (5-6) ◽  
pp. 371-374 ◽  
Author(s):  
R. Gajardo ◽  
R. M. Pintó ◽  
A. Bosch
Keyword(s):  
Polymerase Chain Reaction ◽  
Environmental Samples ◽  
Polymerase Chain Reaction Amplification ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Group A ◽  
Reaction Amplification ◽  
Stool Specimens ◽  
Polymerase Chain

A reverse transcription polymerase chain reaction (RT-PCR) assay is described that has been developed for the detection and serotyping of group A rotavirus in stool specimens and concentrated and non-concentrated sewage specimens.

scholarly journals Prolonged fecal excretion of hepatitis A virus in adult patients with hepatitis A as determined by polymerase chain reaction

Hepatology ◽  
1996 ◽  
Vol 24 (1) ◽  
pp. 10-13 ◽  
Author(s):  
H Yotsuyanagi ◽  
K Koike ◽  
K Yasuda ◽  
K Moriya ◽  
Y Shintani ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Adult Patients ◽  
Fecal Excretion ◽  
Chain Reaction ◽  
Polymerase Chain

Persistence of Hepatitis A Virus in Oysters†

2003 ◽  
Vol 66 (2) ◽  
pp. 331-334 ◽  
Author(s):  
DAVID H. KINGSLEY ◽  
GARY P. RICHARDS
Keyword(s):  
Polymerase Chain Reaction ◽  
Crassostrea Virginica ◽  
Reverse Transcription ◽  
Hepatitis A ◽  
Infectious Virus ◽  
Hepatitis A Virus ◽  
Complete Removal ◽  
Chain Reaction ◽  
Daily Feeding ◽  
Polymerase Chain

We investigated the ability of hepatitis A virus (HAV) to persist for up to 6 weeks in Eastern oysters (Crassostrea virginica). Viral RNA was detected by reverse transcription–polymerase chain reaction 6 weeks after 16 h of exposure to 90,000 PFU (180 PFU/ml of seawater) of HAV. Assaying for infectious virus in oysters that received a daily feeding of phytoplankton recovered 3,800, 650, and 500 PFU of HAV 1, 2, and 3 weeks after contamination with 90,000 PFU of HAV, respectively. However, no infectious HAV was isolated from oysters 4, 5, or 6 weeks after contamination. These results support the position that shellfish depuration is insufficient for the complete removal of infectious viruses. Extended relay times (in excess of 4 weeks) may be required to produce virologically safe shellfish.

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