scholarly journals A cellular isolation system for real-time single-cell oxygen consumption monitoring

2008 ◽  
Vol 5 (suppl_2) ◽  
Author(s):  
Joe Dragavon ◽  
Tim Molter ◽  
Cody Young ◽  
Tim Strovas ◽  
Sarah McQuaide ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Single Cell ◽  
Real Time ◽  
Physiological State ◽  
Isolation System ◽  
Consumption Rates ◽  
Actuator System ◽  
The University ◽  
Oxygen Measurements

The development of a cellular isolation system (CIS) that enables the monitoring of single-cell oxygen consumption rates in real time is presented. The CIS was developed through a multidisciplinary effort within the Microscale Life Sciences Center (MLSC) at the University of Washington. The system comprises arrays of microwells containing Pt-porphyrin-embedded polystyrene microspheres as the reporter chemistry, a lid actuator system and a gated intensified imaging camera, all mounted on a temperature-stabilized confocal microscope platform. Oxygen consumption determination experiments were performed on RAW264.7 mouse macrophage cells as proof of principle. Repeatable and consistent measurements indicate that the oxygen measurements did not adversely affect the physiological state of the cells measured. The observation of physiological rates in real time allows studies of cell-to-cell heterogeneity in oxygen consumption rate to be performed. Such studies have implications in understanding the role of mitochondrial function in the progression of inflammatory-based diseases, and in diagnosing and treating such diseases.

scholarly journals A microwell array device capable of measuring single-cell oxygen consumption rates

2009 ◽  
Vol 135 (2) ◽  
pp. 678-686 ◽  
Author(s):  
Timothy W. Molter ◽  
Sarah C. McQuaide ◽  
Martin T. Suchorolski ◽  
Tim J. Strovas ◽  
Lloyd W. Burgess ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Single Cell ◽  
Microwell Array ◽  
Consumption Rates

scholarly journals A New Approach for Measuring Single-Cell Oxygen Consumption Rates

2008 ◽  
Vol 5 (1) ◽  
pp. 32-42 ◽  
Author(s):  
Timothy W. Molter ◽  
Mark R. Holl ◽  
Joseph M. Dragavon ◽  
Sarah C. McQuaide ◽  
Judith B. Anderson ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Single Cell ◽  
New Approach ◽  
Consumption Rates

Oxygen Consumption by Red Wines. Part I: Consumption Rates, Relationship with Chemical Composition, and Role of SO2

2015 ◽  
Vol 63 (51) ◽  
pp. 10928-10937 ◽  
Author(s):  
Vicente Ferreira ◽  
Vanesa Carrascon ◽  
Mónica Bueno ◽  
Maurizio Ugliano ◽  
Purificación Fernandez-Zurbano
Keyword(s):  
Oxygen Consumption ◽  
Chemical Composition ◽  
Red Wines ◽  
Consumption Rates

scholarly journals ASSESSMENT OF A MICROPLATE SYSTEM FOR MEASURING INDIVIDUAL REAL-TIME RESPIRATION IN SMALL MODEL ORGANISMS OF AGING

2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S918-S919
Author(s):  
Ashley N Turner ◽  
Jessica M Hoffman ◽  
Mickie L Powell ◽  
Melissa J Sammy ◽  
Douglas R Moellering ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Real Time ◽  
Mitochondrial Function ◽  
Genetic Model ◽  
Living Organism ◽  
Preliminary Evidence ◽  
Model Organisms ◽  
Zebrafish Embryos ◽  
Clinical Genetic ◽  
Consumption Rates

Abstract The ability to measure oxygen consumption rates of a living organism in real-time provides an indirect method of monitoring dynamic changes in metabolism reflecting organismal level mitochondrial function. In this study, we assessed the Loligo Systems microplate system for measuring individual respiration in small organisms. This included adult nematodes (Caenorhabditis elegans, N2), zebrafish embryos (Danio rerio, AB), and adult fruit flies (Drosophila melanogaster, w1118). Organisms were placed inside 80 µL glass chambers on a 24-well microplate atop a 24-channel optical fluorescence oxygen reading device. Adult nematodes and zebrafish embryos were in liquid culture, M9 buffer and egg water respectively, and the adult flies were in room air. The microplate and reader were placed inside an incubator for temperature control. A silicone gasket with a thin liner was used to seal the chambers. Reference standard oxygen consumption (respiration) of single and multiple adult nematodes (n=1–4 animals/well), zebrafish embryos (n=1–4 animals/well), and adult flies (n=1–2 animals/well) in the microplate system were achieved. Significant differences across numbers of animals/well and by sex were observed. Validation experiments of the oxygen consumption rates measured in C. elegans in parallel with Seahorse extracellular flux (XF) experiments are underway. The Loligo Systems microplate system offers a non-invasive, non-destructive method to measure real-time respiration in smaller organisms. These data provide preliminary evidence for utility of the system for a variety of biomedical applications that relate to organismal and mitochondrial function/dysfunction, including research in the basic biology of aging in these highly-utilized, pre-clinical, genetic model organisms.

Thermogenesis after a mixed meal: role of leg and splanchnic tissues in men and women

1995 ◽  
Vol 268 (3) ◽  
pp. E433-E438 ◽  
Author(s):  
M. D. Jensen ◽  
C. M. Johnson ◽  
P. E. Cryer ◽  
M. J. Murray
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
Muscle Metabolism ◽  
Mixed Meal ◽  
Relative Contribution ◽  
Energy Needs ◽  
Consumption Rates ◽  
Postprandial Thermogenesis

To determine the relative contribution of splanchnic and leg tissues to postprandial thermogenesis, systemic and regional oxygen consumption rates were measured in nine women and eight men before and for 6 h after the consumption of a mixed meal that provided one-third of the daily energy needs. In women, the increase in splanchnic oxygen uptake accounted for 63 +/- 12% of the postprandial increase in oxygen consumption, whereas in men it accounted for 35 +/- 14% (P = not significant between women and men). Leg oxygen uptake accounted for 11 +/- 4 and 10 +/- 3% of the increase in postprandial oxygen consumption in women and men, respectively. The combined data suggest that approximately 48% of postprandial thermogenesis over 6 h occurs in splanchnic tissues, whereas 30-35% occurs in skeletal muscle. Thus the increase in oxygen consumption after a mixed meal is primarily localized to splanchnic tissues, and major reductions in postprandial thermogenesis are unlikely to be attributable solely to abnormalities of skeletal muscle metabolism.

Role of membrane charge and semiquinone structure on oxygen consumption rates

2002 ◽  
pp. 1823-1828 ◽  
Author(s):  
Antonio E. Alegría ◽  
Lizmarie Rivera ◽  
Emelyn Cordones ◽  
Veronica Castro ◽  
Pedro Sanchez-Cruz
Keyword(s):  
Oxygen Consumption ◽  
Membrane Charge ◽  
Consumption Rates

MATERIAL HANDLING MACHINES AND SYSTEMS – UMI-TWINN PROJECT CONTRIBUTION

2019 ◽  
Vol 12 (1) ◽  
pp. 7-20
Author(s):  
Péter Telek ◽  
Béla Illés ◽  
Christian Landschützer ◽  
Fabian Schenk ◽  
Flavien Massi
Keyword(s):  
Material Handling ◽  
Digital Data ◽  
Flow Process ◽  
Research Directions ◽  
Scientific Excellence ◽  
Research Activities ◽  
New Research ◽  
Eu Project ◽  
The University

Nowadays, the Industry 4.0 concept affects every area of the industrial, economic, social and personal sectors. The most significant changings are the automation and the digitalization. This is also true for the material handling processes, where the handling systems use more and more automated machines; planning, operation and optimization of different logistic processes are based on many digital data collected from the material flow process. However, new methods and devices require new solutions which define new research directions. In this paper we describe the state of the art of the material handling researches and draw the role of the UMi-TWINN partner institutes in these fields. As a result of this H2020 EU project, scientific excellence of the University of Miskolc can be increased and new research activities will be started.

Tertiary English-majored Students’ Perceptions toward the Role of Pronunciation in English Language Learning and their Practicing Strategies

2020 ◽  
Vol 6 (2) ◽  
pp. 264-279
Keyword(s):  
Language Learning ◽  
English Language ◽  
Vital Role ◽  
English Language Learning ◽  
Structured Interviews ◽  
Efl Learners ◽  
The University

Accurate pronunciation has a vital role in English language learning as it can help learners to avoid misunderstanding in communication. However, EFL learners in many contexts, especially at the University of Phan Thiet, still encounter many difficulties in pronouncing English correctly. Therefore, this study endeavors to explore English-majored students’ perceptions towards the role of pronunciation in English language learning and examine their pronunciation practicing strategies (PPS). It involved 155 English-majored students at the University of Phan Thiet who answered closed-ended questionnaires and 18 English-majored students who participated in semi-structured interviews. The findings revealed that students strongly believed in the important role of pronunciation in English language learning; however, they sometimes employed PPS for their pronunciation improvement. Furthermore, the results showed that participants tended to use naturalistic practicing strategies and formal practicing strategies with sounds, but they overlooked strategies such as asking for help and cooperating with peers. Such findings could contribute further to the understanding of how students perceive the role of pronunciation and their PPS use in the research’s context and other similar ones. Received 10th June 2019; Revised 12th March 2020; Accepted 12th April 2020

Sign in / Sign up

Export Citation Format

Share Document