scholarly journals Characterization of RNA synthesis during a one-step growth curve and of the replication mechanism of bovine viral diarrhoea virus

1996 ◽  
Vol 77 (11) ◽  
pp. 2729-2736 ◽  
Author(s):  
Y. Gong ◽  
R. Trowbridge ◽  
T. B. Macnaughton ◽  
E. G. Westaway ◽  
A. D. Shannon ◽  
...  
Keyword(s):  
Growth Curve ◽  
Rna Synthesis ◽  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Replication Mechanism ◽  
Diarrhoea Virus ◽  
One Step ◽  
Step Growth

Detection of antibodies to bovine viral diarrhoea virus (BVDV) and characterization of genomes of BVDV from Brazil

1998 ◽  
Vol 63 (2-4) ◽  
pp. 85-97 ◽  
Author(s):  
Cláudio Wageck Canal ◽  
Marc Strasser ◽  
Christian Hertig ◽  
Aoi Masuda ◽  
Ernst Peterhans
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Diarrhoea Virus

Anti-Bovine Viral Diarrhoea Virus and Hepatitis C Virus Activity of the Cyclooxygenase Inhibitor SC-560

2009 ◽  
Vol 20 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Mika Okamoto ◽  
Masashi Sakai ◽  
Yukinori Goto ◽  
Mohammed TA Salim ◽  
Chiaki Baba ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Antiviral Activity ◽  
Rna Synthesis ◽  
Bovine Viral Diarrhoea Virus ◽  
Inhibitory Effect ◽  
Host Cells ◽  
Bovine Viral Diarrhoea ◽  
Diarrhoea Virus ◽  
Mdbk Cells

Background: A number of compounds were examined for their inhibitory effect on bovine viral diarrhoea virus (BVDV) replication in cell cultures and found that some cyclooxygenase (COX) inhibitors had antiviral activity against the virus. Methods: Determination of compounds for their anti-BVDV activity was on the basis of the inhibition of virus-induced cytopathogenicity in Mardin–Darby bovine kidney (MDBK) cells. Anti-hepatitis C virus (HCV) activity was assessed by the inhibition of viral RNA synthesis in the subgenomic HCV RNA replicon cells. Results: Among the test compounds, 5-(4-chlorophenyl)-1-(4-methoxyphenyl)-3-(trifluoromethyl)-1 H-pyrazole (SC-560) was the most active against BVDV, and its 50% effective and cytotoxic concentrations were 10.9 ±2.8 and 93.9 ±24.5 μM in virus and mock-infected MDBK cells, respectively. The compound also suppressed BVDV RNA synthesis in a dose-dependent fashion. Studies on the mechanism of action revealed that SC-560 did not interfere with viral entry to the host cells. Furthermore, it was assumed that the antiviral activity of SC-560 was not associated with its inhibitory effect on COX. The combination of SC-560 and interferon-α was additive to synergistic in inhibiting BVDV replication. More importantly, the compound proved to be a selective inhibitor of HCV replication. Conclusions: SC-560 and its derivative might have potential as novel antiviral agents against HCV.

Genetic and antigenic characterization of bovine viral diarrhoea virus type 2 isolated from cattle in India

2011 ◽  
Vol 34 (2) ◽  
pp. 189-196 ◽  
Author(s):  
Sthita Pragnya Behera ◽  
Niranjan Mishra ◽  
Stefan Vilcek ◽  
Katherukamem Rajukumar ◽  
Ram Kumar Nema ◽  
...  
Keyword(s):  
Virus Type ◽  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Antigenic Characterization ◽  
Diarrhoea Virus

scholarly journals Quantification of bovine viral diarrhoea virus ribonucleic acid in serum of infected animals by one-step reverse transcriptase quantitative real-time polymerase chain reaction

2019 ◽  
Vol 88 (4) ◽  
pp. 361-367
Author(s):  
Věra Fichtelová ◽  
Kamil Kovařčík
Keyword(s):  
Polymerase Chain Reaction ◽  
Persistent Infection ◽  
Ribonucleic Acid ◽  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Chain Reaction ◽  
Persistently Infected ◽  
Diarrhoea Virus ◽  
One Step ◽  
Polymerase Chain

Bovine viral diarrhoea virus (BVDV) can cause either acute transient or persistent infection. Identification and removal of persistently infected animals from infected herds is a crucial component to control BVDV infection. Only limited data on serum virus concentration in infected animals are available to date. Using one-step reverse transcriptase quantitative real-time polymerase chain reaction, we quantified the serum viral load in 40 BVDV infected animals. To control nucleic acid extraction, complementary DNA synthesis and polymerase chain reaction amplification, each serum sample was spiked with a known small amount of reference canine coronavirus. Detected ribonucleic acid copy number ranged from 2.2 × 106 to 7.4 × 108 per 1 ml of serum of persistently infected animals and from 6.6 × 104 to 3.3 × 107 of transiently infected animals. These findings support the idea that it is impossible to accurately distinguish between transiently and persistently infected animals just from a single blood sample. To use this testing as a means of declining costs of BVDV control programmes cannot be recommended and paired serum samples have to be investigated to confirm persistent infection.

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