scholarly journals Identification of the glycosaminoglycan-binding site on the glycoprotein Erns of bovine viral diarrhoea virus by site-directed mutagenesis

2002 ◽  
Vol 83 (9) ◽  
pp. 2153-2159 ◽  
Author(s):  
Munir Iqbal ◽  
John W. McCauley
Keyword(s):  
Cell Surface ◽  
Binding Site ◽  
Bovine Viral Diarrhoea Virus ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Bovine Viral Diarrhoea ◽  
Surface Binding ◽  
C Terminus ◽  
Diarrhoea Virus ◽  
Lysine Residues

Bovine viral diarrhoea virus (BVDV) envelope glycoprotein Erns interacts with highly sulphated heparin-like glycosaminoglycans (GAGs) located on the cell surface as an early step in virus infection of cells. Site-directed mutagenesis of recombinant Erns was undertaken and analysis of mutants by heparin-affinity chromatography and cell surface binding showed that a cluster of basic amino acids (480KKLENKSK487) near the C terminus of Erns was essential for binding. Mutants with amino acid substitutions of lysine residues 481 and 485 in Erns reduced the binding of Erns to immobilized heparin and cellular GAGs but retained ribonuclease activity. In contrast to normal Erns, Erns that was unable to bind to cells also failed to inhibit BVDV infection of cells when the cells were pre-incubated with Erns. It is proposed that the cluster of basic residues (480KKLENKSK487) localized at the C-terminal end of Erns constitutes a GAG-binding site.

scholarly journals Localization of viral proteins in cells infected with bovine viral diarrhoea virus

2001 ◽  
Vol 82 (11) ◽  
pp. 2597-2605 ◽  
Author(s):  
B. Grummer ◽  
M. Beer ◽  
E. Liebler-Tenorio ◽  
I. Greiser-Wilke
Keyword(s):  
Cell Surface ◽  
Protein Localization ◽  
Bovine Viral Diarrhoea Virus ◽  
Viral Envelope ◽  
Structural Proteins ◽  
Envelope Glycoproteins ◽  
Bovine Viral Diarrhoea ◽  
Diarrhoea Virus ◽  
Viral Glycoproteins ◽  
Infected Cells

Bovine viral diarrhoea virus (BVDV) is a member of the genus Pestivirus within the family Flaviviridae. In this report, protein localization studies were performed to assess the mechanism for the release of mature virus particles from infected cells. Since BVDV is an enveloped virus, budding from either intra- or extracellular membranes is feasible. A prerequisite for the latter mechanism is the integration of viral glycoproteins into the host cell membrane. Using monoclonal antibodies (MAbs) directed against the viral envelope glycoproteins E2 and ERNS, no specific signals were detected on the surface of BVDV-infected cells by indirect fluorescence, confocal microscopy or fluorescence-activated cell sorter analyses. Furthermore, biotin-labelled cell surface proteins of virus-infected and non-infected cells were not detected by immunoprecipitation using MAbs directed against ERNS and E2 or the non-structural protein NS2-3. None of these proteins was detected on the cell surface. In addition, to analyse the intracellular localization of the two viral glycoproteins ERNS and E2 and the non-structural proteins NS2-3 and NS3, subcellular fractionation of virus-infected cells followed by radioimmunoprecipitation with the MAbs were performed. These results led to the conclusion that the BVDV envelope glycoproteins ERNS and E2 as well as the non-structural proteins NS2-3 and NS3 were almost quantitatively associated with intracellular membranes. These findings indicate that BVDV is released by budding into the cisternae of the endoplasmic reticulum and that there seems to be no correlation between the location and function of the analysed proteins.

Pathological lesions of lambs infected in utero with bovine viral diarrhoea virus type 1c (BVDV-1c)

2021 ◽  
Vol 188 (3) ◽  
pp. 187-196
Author(s):  
Caitlin A. Evans ◽  
Lucy Woolford ◽  
Farhid Hemmatzadeh ◽  
Michael P. Reichel ◽  
Peter D. Cockcroft
Keyword(s):  
Virus Type ◽  
Bovine Viral Diarrhoea Virus ◽  
In Utero ◽  
Bovine Viral Diarrhoea ◽  
Pathological Lesions ◽  
Diarrhoea Virus

Bovine viral diarrhoea virus 1b infection associated with congenital tremor and hypomyelination in Holstein calves

2021 ◽  
pp. 109047
Author(s):  
Laura Gallina ◽  
Michel C. Koch ◽  
Arcangelo Gentile ◽  
Ida Treglia ◽  
Cristiano Bombardi ◽  
...  
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Diarrhoea Virus ◽  
Holstein Calves ◽  
Congenital Tremor

A survey for bvdv antibodies in cattle farms in Slovakia and genetic typing of bvdv isolates from imported animals

2003 ◽  
Vol 51 (2) ◽  
pp. 229-236 ◽  
Author(s):  
Š. Vilček ◽  
Jana Mojžišová ◽  
Viera Bajová ◽  
Š. Paulík ◽  
L. Strojný ◽  
...  
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Computer Assisted ◽  
Serological Survey ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Serum Samples ◽  
Genetic Typing ◽  
Diarrhoea Virus ◽  
Pcr Products ◽  
Cattle Farms

A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5´-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1h group.

Anti-Bovine Viral Diarrhoea Virus Antibodies in Adult Zambian Patients Infected with the Human Immunodeficiency Virus

1993 ◽  
Vol 4 (5) ◽  
pp. 300-302 ◽  
Author(s):  
M Giangaspero ◽  
G Vacirca ◽  
D Morgan ◽  
K S Baboo ◽  
N P Luo ◽  
...  
Keyword(s):  
Synergistic Effects ◽  
Bovine Viral Diarrhoea Virus ◽  
Hiv Infections ◽  
Chronic Diarrhoea ◽  
Bovine Viral Diarrhoea ◽  
Diarrhoea Virus ◽  
Immunodeficiency Virus ◽  
Mechanism Of Interaction ◽  
Asymptomatic Individuals ◽  
Hiv Seropositive

Bovine viral diarrhoea (BVD) virus is a cosmopolitan pestivirus of animals which is associated with diarrhoea, immunosuppression and synergy with other pathogens. This study was conducted to establish the prevalence of anti-BVD virus antibodies in healthy Zambian adults and those with asymptomatic and symptomatic HIV disease. Sera from 1159 adults were tested for anti-BVD virus antibodies using the indirect immunofluorescence test and the confirmatory Western blot. Of the 1159 sera examined, 180 (15.5%) showed significantly elevated titres of anti-BVD antibodies. These included 70 out of 477 (14.7%) HIV-negative healthy adults; 73 out of 480 (15.2%) of HIV-positive asymptomatic individuals; 23 out of 129 (17.8%) HIV-seropositive patients with associated illnesses excluding diarrhoea; and 14 out of 73 (19.2%) of HIV-seropositive patients with chronic diarrhoea. HIV-seropositive patients with chronic diarrhoea or associated illnesses appear to have significantly increased seroprevalence of anti-BVD virus antibodies ( P = >0.01). The mechanism of interaction between BVD virus and HIV infections and the synergistic effects with other opportunistic pathogens in humans requires definition.

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