scholarly journals Nutritional efficacy of Chlorella supplementation depends on the individual gut environment: randomized control study

2020 ◽  
Author(s):  
Yuichiro Nishimoto ◽  
Tatsuhiro Nomaguchi ◽  
Yuka Mori ◽  
Masaki Ito ◽  
Yuya Nakamura ◽  
...  
Keyword(s):  
Dicarboxylic Acids ◽  
Blood Parameters ◽  
Integrated Analysis ◽  
Strongly Correlated ◽  
Double Blind ◽  
Intestinal Environment ◽  
Low Concentrations ◽  
Host Diet ◽  
Defecation Frequency ◽  
The Individual

Recent studies have accumulated evidence that the intestinal environment is strongly correlated with host diet, which influences host health. A number of dietary products whose mechanisms of influence operate via the gut microbiota have been revealed, but they are still limited. Here, we investigated the dietary influence of Chlorella, a green alga commercially available as a dietary supplement. A randomized, double-blind, placebo-controlled crossover trial including 40 Japanese participants with constipation was performed and followed by integrated analysis of the gut microbiome, gut metabolome and blood parameters based on a metabologenomics approach. We revealed that the consumption of Chlorella increased the level of several dicarboxylic acids in faeces. Furthermore, the analysis showed that individuals with low concentrations of faecal propionate increased its concentration by Chlorella intake. In addition, increasing of blood folate levels were negatively correlated with defecation frequency at baseline. Our study suggested that the effect of Chlorella consumption varies by individuals depending on their intestinal environment, which illustrates the importance of stratified dietary management based on the intestinal environment in individuals.

scholarly journals The Nutritional Efficacy of Chlorella Supplementation Depends on the Individual Gut Environment: A Randomised Control Study

2021 ◽  
Vol 8 ◽  
Author(s):  
Yuichiro Nishimoto ◽  
Tatsuhiro Nomaguchi ◽  
Yuka Mori ◽  
Masaki Ito ◽  
Yuya Nakamura ◽  
...  
Keyword(s):  
Dicarboxylic Acids ◽  
Blood Parameters ◽  
Secondary Outcome ◽  
Strongly Correlated ◽  
Double Blind ◽  
Intestinal Environment ◽  
Low Concentrations ◽  
Host Diet ◽  
Defecation Frequency ◽  
The Individual

Recent studies have accumulated evidence that the intestinal environment is strongly correlated with host diet, which influences host health. A number of dietary products whose mechanisms of influence operate via the gut microbiota have been revealed, but they are still limited. Here, we investigated the dietary influence of Chlorella, a green alga commercially available as a dietary supplement. A randomised, double-blind, placebo-controlled crossover trial including 40 Japanese participants with constipation was performed. In this study, the primary outcome and secondary outcome were set as defecation frequency and blood folate level, respectively. In both outcomes, no significant differences were detected compared to the control intake. Therefore, we analysed the gut microbiome, gut metabolome, and blood parameters in an integrated manner as an exploratory analysis. We revealed that the consumption of Chlorella increased the level of several dicarboxylic acids in faeces. Furthermore, the analysis showed that individuals with low concentrations of faecal propionate showed an increase in propionate concentration upon Chlorella intake. In addition, increasing blood folate levels were negatively correlated with defecation frequency at baseline. Our study suggested that the effect of Chlorella consumption varies among individuals depending on their intestinal environment, which illustrates the importance of stratified dietary management based on the intestinal environment in individuals.

scholarly journals Refined characterization of circulating tumor DNA through biological feature integration

2021 ◽  
Author(s):  
Havell Markus ◽  
Dineika Chandrananda ◽  
Elizabeth Moore ◽  
Florent Mouliere ◽  
James Morris ◽  
...  
Keyword(s):  
Cancer Patients ◽  
Fragment Size ◽  
Circulating Tumor Dna ◽  
Integrated Analysis ◽  
Fragmentation Pattern ◽  
Nucleosome Core ◽  
Low Concentrations ◽  
Healthy State ◽  
The Individual ◽  
Tumor Dna

Circulating tumor DNA (ctDNA) in blood plasma is present at very low concentrations compared to cell-free DNA (cfDNA) of non-tumor origin. To enhance detection, recent studies have been focused on understanding the non-random fragmentation pattern of cfDNA. These studies have investigated fragment sizes, genomic position of fragment end points, and fragment end motifs. Although these features have been described and shown to be aberrant in cancer patients, there is a lack of understanding of how the individual and integrated analysis of these features enrich ctDNA fraction and enhance ctDNA detection. Using whole genome sequencing and copy number analysis of plasma samples from 5 high-grade serious ovarian cancer patients, we observed that 1)ctDNA is enriched not only in fragments shorter than mono-nucleosomes (~167bp), but also in those shorter than di-nucleosomes (~240-330bp) (28-159% enrichment). 2)fragments that start and end at the border or within the nucleosome core are enriched in ctDNA (5-46% enrichment). 3)certain DNA motifs conserved in regions 10bp up- and down- stream of fragment ends (i.e. cleavage sites) could be used to detect tumor-derived fragments (10-44% enrichment). We further show that the integrated analysis of these three features resulted in a higher enrichment of ctDNA when compared to using fragment size alone (additional 7-25% enrichment after fragment size selection). We believe these genome wide features, which are independent of genetic mutational changes, could allow new ways to analyze and interpret cfDNA data, as significant aberrations of these features from a healthy state could improve its utility as a diagnostic biomarker.

scholarly journals Kinetical Study, Thermo-Mechanical Characteristics and Recyclability of Epoxidized Camelina Oil Cured with Antagonist Structure (Aliphatic/Aromatic) or Functionality (Acid/Amine) Hardeners

Polymers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2503
Author(s):  
Chiara Di Mauro ◽  
Aratz Genua ◽  
Alice Mija
Keyword(s):  
Disulfide Bonds ◽  
Mechanical Characteristics ◽  
Dicarboxylic Acids ◽  
Thermomechanical Properties ◽  
Chemical Recycling ◽  
Camelina Oil ◽  
Ft Ir ◽  
Tan Δ ◽  
The Individual ◽  
First Time

In an attempt to prepare sustainable epoxy thermosets, this study introduces for the first time the idea to use antagonist structures (aromatic/aliphatic) or functionalities (acid/amine) as hardeners to produce reprocessable resins based on epoxidized camelina oil (ECMO). Two kinds of mixtures were tested: one combines aromatic/aliphatic dicarboxylic acids: 2,2′-dithiodibenzoic acid (DTBA) and 3,3′-dithiodipropionic acid (DTDA); another is the combination of two aromatic structures with acid/amine functionality: DTBA and 4-aminophenyl disulfide (4-AFD). DSC and FT-IR analyses were used as methods to analyze the curing reaction of ECMO with the hardeners. It was found that the thermosets obtained with the dual crosslinked mechanism needed reduced curing temperatures and reprocessing protocols compared to the individual crosslinked thermosets. Thanks to the contribution of disulfide bonds in the network topology, the obtained thermosets showed recycling ability. The final thermomechanical properties of the virgin and mechanical reprocessed materials were analyzed by DMA and TGA. The obtained thermosets range from elastomeric to rigid materials. As an example, the ECMO/DTBA704-AFD30 virgin or reprocessed thermosets have tan δ values reaching 82–83 °C. The study also investigates the chemical recycling and the solvent resistance of these vitrimer-like materials.

scholarly journals Characteristic Microbiomes Correlate with Polyphosphate Accumulation of Marine Sponges in South China Sea Areas

2019 ◽  
Vol 8 (1) ◽  
pp. 63
Author(s):  
Huilong Ou ◽  
Mingyu Li ◽  
Shufei Wu ◽  
Linli Jia ◽  
Russell T. Hill ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Inorganic Phosphorus ◽  
Marine Sponges ◽  
Strongly Correlated ◽  
Mantel Tests ◽  
Low Concentrations ◽  
Enrichment Rate ◽  
Total Dna ◽  
Polyphosphate Accumulation ◽  
Sequence Similarities

Some sponges have been shown to accumulate abundant phosphorus in the form of polyphosphate (polyP) granules even in waters where phosphorus is present at low concentrations. But the polyP accumulation occurring in sponges and their symbiotic bacteria have been little studied. The amounts of polyP exhibited significant differences in twelve sponges from marine environments with high or low dissolved inorganic phosphorus (DIP) concentrations which were quantified by spectral analysis, even though in the same sponge genus, e.g., Mycale sp. or Callyspongia sp. PolyP enrichment rates of sponges in oligotrophic environments were far higher than those in eutrophic environments. Massive polyP granules were observed under confocal microscopy in samples from very low DIP environments. The composition of sponge symbiotic microbes was analyzed by high-throughput sequencing and the corresponding polyphosphate kinase (ppk) genes were detected. Sequence analysis revealed that in the low DIP environment, those sponges with higher polyP content and enrichment rates had relatively higher abundances of cyanobacteria. Mantel tests and canonical correspondence analysis (CCA) examined that the polyP enrichment rate was most strongly correlated with the structure of microbial communities, including genera Synechococcus, Rhodopirellula, Blastopirellula, and Rubripirellula. About 50% of ppk genes obtained from the total DNA of sponge holobionts, had above 80% amino acid sequence similarities to those sequences from Synechococcus. In general, it suggested that sponges employed differentiated strategies towards the use of phosphorus in different nutrient environments and the symbiotic Synechococcus could play a key role in accumulating polyP.

scholarly journals Responsiveness of the Scripps Neurologic Rating Scale During a Multiple Sclerosis Clinical Trial

1999 ◽  
Vol 26 (4) ◽  
pp. 283-289 ◽  
Author(s):  
James A. Koziol ◽  
Adriana Lucero ◽  
Jack C. Sipe ◽  
John S. Romine ◽  
Ernest Beutler
Keyword(s):  
Multiple Sclerosis ◽  
Clinical Trial ◽  
Rating Scale ◽  
Effect Sizes ◽  
Active Therapy ◽  
Double Blind ◽  
Treatment Groups ◽  
Negative Effect ◽  
The Individual ◽  
Positive Effect

Objective:The Scripps neurologic rating scale (SNRS) is a summary measure of individual components comprising a neurological examination, designed for use in multiple sclerosis (MS). Our objective is to evaluate the responsiveness of the SNRS, within the context of a 2-year, randomized, double-blind crossover study of the efficacy of cladribine for treatment of secondary progressive MS.Methods:Effect sizes were determined for the SNRS and its components, separately for each treatment group (initial placebo, and initial cladribine) over both years of the clinical trial, using a standard random effects model.Results:Individual components tended to show positive effect sizes (improvement) during periods of active therapy in both treatment groups, and negative effect sizes (deterioration) during periods of no active therapy. Summation indices derived from the individual components of the SNRS seemed somewhat more stable than the individual components. The two components mentation and mood, and bladder, bowel, or sexual dysfunction, were rather unresponsive in our clinical trial.Conclusion:Changes in the components of the SNRS over the course of our clinical trial were consistent between the two treatment groups. Most components were moderately responsive; and, the summary SNRS score appropriately summarized the moderate magnitudes of change evinced in the individual components.

The effect of fungal colonization on the morphine production of poppy (Papaver somniferum L.) capsules

1982 ◽  
Vol 98 (3) ◽  
pp. 679-687 ◽  
Author(s):  
J. C. Laughlin ◽  
D. Munro
Keyword(s):  
Field Experiment ◽  
Papaver Somniferum ◽  
Fungal Colonization ◽  
Capsule Wall ◽  
Surface Cover ◽  
Morphine Concentration ◽  
Commercial Harvest ◽  
Low Concentrations ◽  
The Individual

SUMMARYAbnormally low concentrations of morphine in capsules of poppy (Papaver somniferum L.) in the 1970–1 season were associated with heavy fungal colonization. The effect of fungal colonization on the morphine production of capsules was later studied in a series of field, glasshouse and in vitroexperiments.In a field experiment morphine concentration of severely colonized (> 30% surface cover) intact capsules was 20% less (P <0·01) than slightly colonized (< 10% surface cover) capsules. Colonization of these field-grown capsules was generally localized in the top half and the morphine concentration of the top half was about 20% less than the bottom half for all colonization categories. In contrast, glasshouse-grown capsules were free of fungal colonization and the top and bottom halves had similar morphine concentrations.In a field experiment studying the effect of fungicides, 2 kg benomyl (50% a.i.) + 2 kg mancozeb (80% a.i.)/ha were applied as a spray at 10-day intervals from flowering till 1 month after commercial harvest and plants were harvested at weekly intervals from 10 days after full bloom. The mean dry-matter yield of sprayed capsules was 11% greater (P <0·01) than non-sprayed with a similar trend for morphine concentration and morphine yield. In addition, the sprayed treatment significantly reduced the area covered by sporulating lesions on the surface of the capsule after dry maturity. This superficial fungal cover had a NNE orientation in both sprayed and non-sprayed capsules.In an in vitroexperiment using capsules from the field fungicide study, fungi were isolated from the interior of green capsule wall tissue as early as 17 days after flowering. Colonization increased with successive harvests and culturing of fungi from the interior of capsule wall tissue showed the presence of fungi in both sprayed and non-sprayed capsules with no difference in the degree of colonization.Two of the major fungi isolated from the field experiment were identified as Dendryphion penidllatum (Corda) Fr. and Alternaria alternata (Fr.) Keissler and the individual effect of these was assessed in an in vitro experiment using ground capsule material. D. penidllatuvi and A. alternata reduced the morphine concentration of ground capsules in 24 days to 7 and 11% respectively of non-inoculated controls.

Using Activities of Daily Living Assessments to Measure the Effectiveness of Vitamin D Supplements in Elderly Long-Stay Patients

1987 ◽  
Vol 50 (2) ◽  
pp. 60-62 ◽  
Author(s):  
D Corless ◽  
M Ellis ◽  
E Dawson ◽  
F Fraser ◽  
S Evans ◽  
...  
Keyword(s):  
Vitamin D ◽  
Elderly Patients ◽  
Activities Of Daily Living ◽  
Daily Living ◽  
Controlled Trial ◽  
Double Blind ◽  
Hydroxyvitamin D ◽  
Low Concentrations ◽  
25 Hydroxyvitamin D ◽  
Vitamin D Supplements

Selected activities of daily living were used to measure improvement in independence of long-stay elderly patients known to have low concentrations of plasma 25-hydroxyvitamin D. This was a double-blind random controlled trial lasting between 8 and 40 weeks. No significant changes were found in either group.

scholarly journals The role of bovine colostrum on recovery time and length of hospital stay of acute diarrhea in infants and children: a double-blind randomized controlled trial

2016 ◽  
Vol 46 (3) ◽  
pp. 127
Author(s):  
IGN Suwarba ◽  
Sudaryat S ◽  
Hendra S ◽  
IKG Suandi ◽  
Raka Widiana
Keyword(s):  
Hospital Stay ◽  
Acute Diarrhea ◽  
Recovery Time ◽  
Controlled Trial ◽  
Length Of Hospital Stay ◽  
Bovine Colostrum ◽  
Mean Difference ◽  
Infants And Children ◽  
Double Blind ◽  
Defecation Frequency

Background WHO standard treatment for acute diarrhea remainsunsatisfying to the parents of acute diarrhea patients, particularlythe need of medical treatment. Bovine colostrum contains immuneand growth factors that is thought able to neutralize some agentscausing acute diarrhea in infants and children.Objective To evaluate the efficacy of bovine colostrum as adju-vant therapy on recovery time and length of hospital stay for acutediarrhea in infants and children.Methods A double-blind randomized controlled trial was conductedon infants and children with acute diarrhea admitted to SanglahHospital. Treatment group received standard therapy with bovinecolostrums and control group received standard therapy plus pla-cebo. The primary outcomes were achievement of recovery timeand length of hospital stay. Recovery time was determined by thenumber of days needed to achieve defecation frequency <3 times/day and needed to achieve normal stool consistency.Results Seventy infants and children were enrolled. The treatmentgroup significantly achieved recovery time earlier than the control groupin regard to the time of achieving defecation frequency to <3 times/day [2.31 (0.76) vs 3.34 (1.45); mean difference of -1.03; P= 0.001; CI95% -1.58;-0.48] and normal stool consistency [2.40 (0.77) vs 3.43(1.48); mean difference of -1.03; P = 0.001; CI 95% -1.59;-0.46]. Lengthof hospital stay was shorter in the treatment group than the controlgroup [2.89 (0.78) vs 3.94 (1.53); mean difference of -1.05; P= 0.001;CI 95% (-1.3;-0.7)]. No significant difference was found in mean ofbody weight recovery in two groups [0.47 (0.16) vs 0.49 (0.20); meandifference of -0.03; P=0.556; CI 95%: -0.11;0.06]. Age, nutritionalstatus, breastfeeding, and diarrhea before admission did not influ-ence the study outcome.Conclusion Bovine colostrums as an adjuvant in standard therapyfor acute diarrhea in infants and children is effective in regard toachieve earlier recovery time and shorter length of hospital stay

scholarly journals Effect of Bifidobacterium longum BB536 Administration on the Intestinal Environment, Defecation Frequency and Fecal Characteristics of Human Volunteers

1997 ◽  
Vol 16 (2) ◽  
pp. 53-58 ◽  
Author(s):  
Tomohiro OGATA ◽  
Teiichi NAKAMURA ◽  
Katsue ANJITSU ◽  
Tomoko YAESHIMA ◽  
Sachiko TAKAHASHI ◽  
...  
Keyword(s):  
Bifidobacterium Longum ◽  
Intestinal Environment ◽  
Human Volunteers ◽  
Defecation Frequency

scholarly journals The impact of the artery geometry with the sewn surgical patch on the blood flow disorders

2019 ◽  
Vol 128 ◽  
pp. 02005
Author(s):  
Natalia Lewandowska ◽  
Michal Cialkowski
Keyword(s):  
Blood Flow ◽  
Input Data ◽  
Polynomial Function ◽  
Blood Parameters ◽  
Atherosclerotic Plaques ◽  
Fluid Density ◽  
The Common ◽  
The Individual ◽  
The Impact ◽  
Patch Length

The research concerns the development of geometric variants of patches sewn into the common carotid artery during surgery of the atherosclerotic plaques removal. Based on analytical methods, thegeometry of the patch described by the polynomial function has been developed. The simulations of blood flow in the arteries with the sewn patch were performed. The study included the influence of the patient’s diameter and the width of the chosen patch on blood flow disorders. The result of the research is the algorithm of selecting the geometry of the arterial patch to the individual geometrical featuresof the patient’s artery. The studies result will comprise the development of software, which, upon introduction of input data related to arterial geometry, patch length and patient’s blood parameters (affecting the fluid density and viscosity), shall generate an accurate contour of the patch of width causing no flow disorders.

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