scholarly journals TACSTD2 upregulation is an early reaction to lung infection

2021 ◽  
Author(s):  
Sara Lenart ◽  
Peter Lenart ◽  
Hana Kotasova ◽  
Vendula Pelkova ◽  
Veronika Sedlakova ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Physiological Role ◽  
Lung Epithelial Cells ◽  
Antibody Drug Conjugate ◽  
Epithelial Barrier Function ◽  
Transcriptomic Data ◽  
Transmembrane Glycoprotein ◽  
Lung Epithelial ◽  
Evolutionarily Conserved

TACSTD2 encodes a transmembrane glycoprotein Trop2 commonly overexpressed in carcinomas. While the Trop2 protein was discovered already in 1981 and first antibody-drug conjugate targeting Trop2 were recently approved cancer therapy, the physiological role of Trop2 is still not fully understood. In this article, we show that TACSTD2/Trop2 expression is evolutionarily conserved in lungs of various vertebrates. By analysis of publicly available transcriptomic data we demonstrate that TACSTD2 level consistently increases in lungs infected with miscellaneous pathogens. Single cell and subpopulation based transcriptomic data revealed that the major source of TACSTD2 transcript are lung epithelial cells and their progenitors and that TACSTD2 is induced directly in lung epithelial cells following infection. This increase may represent a mechanism to maintain/restore epithelial barrier function and contribute to regeneration process in infected/damaged lungs.

MC1568 inhibits HDAC6/8 activity and influenza A virus replication in lung epithelial cells: role of Hsp90 acetylation

2016 ◽  
Vol 8 (17) ◽  
pp. 2017-2031 ◽  
Author(s):  
Simona Panella ◽  
Maria Elena Marcocci ◽  
Ignacio Celestino ◽  
Sergio Valente ◽  
Clemens Zwergel ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Influenza A Virus ◽  
Virus Replication ◽  
Influenza A ◽  
Lung Epithelial Cells ◽  
Lung Epithelial

scholarly journals HIV-1 Productively Infects and Integrates in Bronchial Epithelial Cells

2021 ◽  
Vol 10 ◽  
Author(s):  
Dinesh Devadoss ◽  
Shashi P. Singh ◽  
Arpan Acharya ◽  
Kieu Chinh Do ◽  
Palsamy Periyasamy ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Bronchial Epithelial Cells ◽  
Lung Epithelial Cells ◽  
People Living With Hiv ◽  
Sequencing Analysis ◽  
Hiv Reservoirs ◽  
Bronchial Epithelial ◽  
Lung Epithelial ◽  
Hiv 1

BackgroundThe role of lung epithelial cells in HIV-1-related lung comorbidities remains unclear, and the major hurdle in curing HIV is the persistence of latent HIV reservoirs in people living with HIV (PLWH). The advent of combined antiretroviral therapy has considerably increased the life span; however, the incidence of chronic lung diseases is significantly higher among PLWH. Lung epithelial cells orchestrate the respiratory immune responses and whether these cells are productively infected by HIV-1 is debatable.MethodsNormal human bronchial epithelial cells (NHBEs) grown on air–liquid interface were infected with X4-tropic HIV-1LAV and examined for latency using latency-reversing agents (LRAs). The role of CD4 and CXCR4 HIV coreceptors in NHBEs were tested, and DNA sequencing analysis was used to analyze the genomic integration of HIV proviral genes, Alu-HIVgag-pol, HIV-nef, and HIV-LTR. Lung epithelial sections from HIV-infected humans and SHIV-infected macaques were analyzed by FISH for HIV-gag-pol RNA and epithelial cell-specific immunostaining.Results and DiscussionNHBEs express CD4 and CXCR4 at higher levels than A549 cells. NHBEs are infected with HIV-1 basolaterally, but not apically, by X4-tropic HIV-1LAV in a CXCR4/CD4-dependent manner leading to HIV-p24 antigen production; however, NHBEs are induced to express CCR5 by IL-13 treatment. In the presence of cART, HIV-1 induces latency and integration of HIV provirus in the cellular DNA, which is rescued by the LRAs (endotoxin/vorinostat). Furthermore, lung epithelial cells from HIV-infected humans and SHIV-infected macaques contain HIV-specific RNA transcripts. Thus, lung epithelial cells are targeted by HIV-1 and could serve as potential HIV reservoirs that may contribute to the respiratory comorbidities in PLWH.

scholarly journals Role of protein kinase C in cytokine secretion by lung epithelial cells during infection withParacoccidioides brasiliensis

2015 ◽  
Vol 73 (7) ◽  
pp. ftv045 ◽  
Author(s):  
Cristiane Alcantara ◽  
Paloma Korehisa Maza ◽  
Bianca Carla Silva Campitelli Barros ◽  
Erika Suzuki
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Cytokine Secretion ◽  
Kinase C ◽  
Lung Epithelial

Expression and role of E- and P-cadherin adhesion molecules in embryonic histogenesis. I. Lung epithelial morphogenesis

Development ◽  
1989 ◽  
Vol 105 (2) ◽  
pp. 263-270 ◽  
Author(s):  
Y. Hirai ◽  
A. Nose ◽  
S. Kobayashi ◽  
M. Takeichi
Keyword(s):  
Monoclonal Antibody ◽  
Cell Adhesion ◽  
Epithelial Cells ◽  
Adhesion Molecules ◽  
Lung Epithelial Cells ◽  
Early Developmental Stage ◽  
Subtle Effect ◽  
Lung Epithelial ◽  
Cell Cell

The role of Ca2+-dependent cell-cell adhesion molecules, E- and P-cadherins, in the histogenesis of mouse embryonic lung was studied. All epithelial cells of the lung express both E- and P-cadherin at the early developmental stage. P-cadherin, however, gradually disappears during development, initially from the main bronchi and eventually from all epithelial cells. When a monoclonal antibody to E-cadherin (ECCD-1) was added to monolayer cultures of lung epithelial cells, it induced a partial disruption of their cell-cell adhesion, while a monoclonal antibody to P-cadherin (PCD-1) showed a subtle effect. A mixture of the two antibodies, however, displayed a synergistic effect. We then tested the effect of the antibodies on the morphogenesis of lung primordia using an organ culture system. In control media, the explants formed typical bronchial trees. In the presence of ECCD-1, the explants grew up at the same rate as in the control, but their morphogenesis was affected. The control explants formed round epithelial lobules with an open luminal space at the tips of the bronchial trees, whereas the lobules of explants incubated with ECCD-1 tended to be flat and devoid of the luminal space. PCD-1 showed a similar but very small effect. A mixture of the two antibodies, however, showed a stronger effect: the branching of epithelia was partially suppressed and the arrangement of epithelial cells was distorted in many places. These results suggest that E- and P-cadherin have a synergistic role in the organization of epithelial cells in lung morphogenesis.

scholarly journals Role of human neutrophil peptides in the initial interaction between lung epithelial cells and CD4+lymphocytes

2007 ◽  
Vol 81 (4) ◽  
pp. 1022-1031 ◽  
Author(s):  
Rosanna Vaschetto ◽  
Jonathan Grinstein ◽  
Lorenzo Del Sorbo ◽  
Aye Aye Khine ◽  
Stefanos Voglis ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Neutrophil ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Initial Interaction ◽  
Cd4 Lymphocytes

The Potential Role of Lung Epithelial Cells and β-defensins in Experimental Latent Tuberculosis

2008 ◽  
Vol 67 (5) ◽  
pp. 448-452 ◽  
Author(s):  
B. Rivas-Santiago ◽  
J. C. L. Contreras ◽  
E. Sada ◽  
R. Hernández-Pando
Keyword(s):  
Epithelial Cells ◽  
Potential Role ◽  
Latent Tuberculosis ◽  
Lung Epithelial Cells ◽  
Lung Epithelial

Dual role of TGF-β1 on Fas-induced apoptosis in lung epithelial cells

2011 ◽  
Vol 177 (3) ◽  
pp. 241-246 ◽  
Author(s):  
Li Bai ◽  
Zubin Yu ◽  
Changzheng Wang ◽  
Guisheng Qian ◽  
Guansong Wang
Keyword(s):  
Epithelial Cells ◽  
Dual Role ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Induced Apoptosis ◽  
Tgf Β1
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