scholarly journals Live imaging YAP signaling in vivo with fusion reporter mice

2021 ◽  
Author(s):  
Bin Gu ◽  
Brian Bradshaw ◽  
Min Zhu ◽  
Yu Sun ◽  
Sevan Hopyan ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Cell Polarity ◽  
Near Infrared ◽  
Kinase Activity ◽  
Live Imaging ◽  
Mouse Line ◽  
Reporter Mouse ◽  
Reporter Mice ◽  
Mammalian Embryonic Development

YAP protein is a critical regulator of mammalian embryonic development. By generating a near-infrared fusion YAP reporter mouse line, we have achieved high-resolution live imaging of YAP localization during mouse embryonic development. We have validated the reporter by demonstrating its predicted responses to blocking Lats kinase activity or blocking cell polarity. The YAP fusion reporter mice and imaging methods will open new opportunities for understanding dynamic YAP signaling in vivo in many different situations.

scholarly journals Tissue fluidity mediated by adherens junction dynamics promotes planar cell polarity-driven ommatidial rotation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nabila Founounou ◽  
Reza Farhadifar ◽  
Giovanna M. Collu ◽  
Ursula Weber ◽  
Michael J. Shelley ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cell Polarity ◽  
Planar Cell Polarity ◽  
Adherens Junction ◽  
Live Imaging ◽  
Cellular Migration ◽  
Imaging Analysis ◽  
Ommatidial Rotation ◽  
Eye Morphogenesis

AbstractThe phenomenon of tissue fluidity—cells’ ability to rearrange relative to each other in confluent tissues—has been linked to several morphogenetic processes and diseases, yet few molecular regulators of tissue fluidity are known. Ommatidial rotation (OR), directed by planar cell polarity signaling, occurs during Drosophila eye morphogenesis and shares many features with polarized cellular migration in vertebrates. We utilize in vivo live imaging analysis tools to quantify dynamic cellular morphologies during OR, revealing that OR is driven autonomously by ommatidial cell clusters rotating in successive pulses within a permissive substrate. Through analysis of a rotation-specific nemo mutant, we demonstrate that precise regulation of junctional E-cadherin levels is critical for modulating the mechanical properties of the tissue to allow rotation to progress. Our study defines Nemo as a molecular tool to induce a transition from solid-like tissues to more viscoelastic tissues broadening our molecular understanding of tissue fluidity.

scholarly journals Effect of Bacterial Endotoxins on Superovulated Mouse Embryos In Vivo: Is CSF-1 Involved in Endotoxin-Induced Pregnancy Loss?

2006 ◽  
Vol 2006 ◽  
pp. 1-9 ◽  
Author(s):  
Yogesh Kumar Jaiswal ◽  
Madan Mohan Chaturvedi ◽  
Kaushik Deb
Keyword(s):  
Embryonic Development ◽  
Pregnancy Loss ◽  
Mouse Embryos ◽  
Preimplantation Embryos ◽  
Implantation Failure ◽  
Normal Pattern ◽  
Bacterial Endotoxins ◽  
Mammalian Embryonic Development ◽  
Lps Treatment

Mammalian embryonic development is regulated by several cytokines and growth factors from embryonic or maternal origins. Since CSF-1 plays important role in embryonic development and implantation, we investigated its role in gram-negative bacterial LPS-induced implantation failure. The effect of LPS on normal (nonsuperovulated) and superovulated in vivo-produced embryos was assessed by signs of morphological degeneration. A significantly similar number of morphologically degenerated embryos recovered from both nonsuperovulated and superovulated LPS treated animals on day 2.5 of pregnancy onwards were morphologically and developmentally abnormal as compared to their respective controls (P<.001. Normal CSF-1 expression level and pattern were also altered through the preimplantation period in the mouse embryos and uterine horns after LPS treatment. This deviation from the normal pattern and level of CSF-1 expression in the preimplantation embryos and uterine tissues suggest a role for CSF-1 in LPS-induced implantation failure.

scholarly journals The mito::mKate2 mouse: A far-red fluorescent reporter mouse line for tracking mitochondrial dynamics in vivo

genesis ◽  
2017 ◽  
Vol 56 (2) ◽  
pp. e23087 ◽  
Author(s):  
Anthony P. Barrasso ◽  
Xuefei Tong ◽  
Ross A. Poché
Keyword(s):  
Mitochondrial Dynamics ◽  
Mouse Line ◽  
Fluorescent Reporter ◽  
Reporter Mouse

scholarly journals Generation and live imaging of an endogenous Cdx2 reporter mouse line

genesis ◽  
2012 ◽  
Vol 50 (10) ◽  
pp. 775-782 ◽  
Author(s):  
Katie McDole ◽  
Yixian Zheng
Keyword(s):  
Live Imaging ◽  
Mouse Line ◽  
Reporter Mouse

scholarly journals Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter mouse strains in vitro and in vivo

Haematologica ◽  
2021 ◽  
Author(s):  
Leo Nicolai ◽  
Rainer Kaiser ◽  
Raphael Escaig ◽  
Marie-Louise Hoffknecht ◽  
Afra Anjum ◽  
...  
Keyword(s):  
Single Cell ◽  
Platelet Function ◽  
Cell Tracking ◽  
Mouse Strains ◽  
Single Cell Level ◽  
Cell Level ◽  
Reporter Mouse ◽  
Reporter Mice

Visualizing cell behavior and effector function on a single cell level has been crucial for understanding key aspects of mammalian biology. Due to their small size, large number and rapid recruitment into thrombi, there is a lack of data on fate and behavior of individual platelets in thrombosis and hemostasis. Here we report the use of platelet lineage restricted multi-color reporter mouse strains to delineate platelet function on a single cell level. We show that genetic labeling allows for single platelet and megakaryocyte tracking and morphological analysis in vivo and in vitro, while not affecting lineage functions. Using Credriven Confetti expression, we provide insights into temporal gene expression patterns as well as spatial clustering of megakaryocytes in the bone marrow. In the vasculature, shape analysis of activated platelets recruited to thrombi identifies ubiquitous filopodia formation with no evidence of lamellipodia formation. Single cell tracking in complex thrombi reveals prominent myosin-dependent motility of platelets and highlights thrombus formation as a highly dynamic process amenable to modification and intervention of the acto-myosin cytoskeleton. Platelet function assays combining flow cytrometry, as well as in vivo, ex vivo and in vitro imaging show unaltered platelet functions of multicolor reporter mice compared to WT controls. In conclusion, platelet lineage multicolor reporter mice prove useful in furthering our understanding of platelet and megakaryocyte biology on a single cell level.

scholarly journals A novel mouse line with epididymal initial segment-specific expression of Cre recombinase driven by the endogenous Lcn9 promoter

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254802
Author(s):  
Qian-qian Gong ◽  
Xiao Wang ◽  
Zhi-lin Dou ◽  
Ke-yi Zhang ◽  
Xiang-guo Liu ◽  
...  
Keyword(s):  
Initial Segment ◽  
Stop Codon ◽  
Dna Recombination ◽  
Cre Recombinase ◽  
Functional Study ◽  
The Novel ◽  
Mouse Line ◽  
Specific Expression ◽  
Reporter Mice

Spermatozoa released from testes undergo a maturation process and acquire the capacity to fertilize ova through epididymal transit. The epididymis is divided into four regions, each with unique morphology, gene profile, luminal microenvironment and distinct function. To study the functions of relevant genes in the epididymal initial segment (IS), a novel IS-specific mouse model, Lcn9-Cre knock-in (KI) mouse line was generated via CRISPR/Cas9 technology. The TAG stop codon was replaced by a 2A-NLS-Cre cassette, resulting in the co-expression of Lcn9 and Cre recombinase. IS-specific Cre expression was first observed from postnatal day 17. Using the Rosa26tdTomato reporter mice, the Cre-mediated DNA recombination was detected exclusively in principal cells. The epididymal IS-specific Cre activity in vivo was further confirmed using Lcn9-Cre mice crossed with a mouse strain carrying Tsc1 floxed alleles (Tsc1flox/+). Cre expression did not affect either normal development or male fecundity. Different from any epididymis-specific Cre mice reported previously, the novel Lcn9-Cre mouse line can be used to introduce entire IS-specific conditional gene editing for gene functional study.

scholarly journals Live imaging of follicle stimulating hormone receptors in gonads and bones using near infrared II fluorophore

2017 ◽  
Vol 8 (5) ◽  
pp. 3703-3711 ◽  
Author(s):  
Yi Feng ◽  
Shoujun Zhu ◽  
Alexander L. Antaris ◽  
Hao Chen ◽  
Yuling Xiao ◽  
...  
Keyword(s):  
Hormone Receptors ◽  
Near Infrared ◽  
Follicle Stimulating Hormone ◽  
Live Imaging ◽  
Hormonal Control ◽  
Stimulating Hormone

In vivoimaging of hormone receptors provides the opportunity to visualize target tissues under hormonal control in live animals.

scholarly journals Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo

2014 ◽  
Vol 2 ◽  
Author(s):  
Yoshinori Makino ◽  
Erina Inoue ◽  
Masashi Hada ◽  
Keisuke Aoshima ◽  
Satsuki Kitano ◽  
...  
Keyword(s):  
Mouse Line ◽  
Reporter Mouse ◽  
Dual Color

scholarly journals The LRRK2-related Roco kinase Roco2 is regulated by Rab1A and controls the actin cytoskeleton

2011 ◽  
Vol 22 (13) ◽  
pp. 2198-2211 ◽  
Author(s):  
Sebastian Kicka ◽  
Zhouxin Shen ◽  
Sarah J. Annesley ◽  
Paul R. Fisher ◽  
Susan Lee ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Cell Polarity ◽  
Actin Polymerization ◽  
Kinase Activity ◽  
Cell Polarization ◽  
Small Gtpase ◽  
Actin Binding ◽  
Leucine Rich Repeat ◽  
Mediate Cell

We identify a new pathway that is required for proper pseudopod formation. We show that Roco2, a leucine-rich repeat kinase 2 (LRRK2)-related Roco kinase, is activated in response to chemoattractant stimulation and helps mediate cell polarization and chemotaxis by regulating cortical F-actin polymerization and pseudopod extension in a pathway that requires Rab1A. We found that Roco2 binds the small GTPase Rab1A as well as the F-actin cross-linking protein filamin (actin-binding protein 120, abp120) in vivo. We show that active Rab1A (Rab1A-GTP) is required for and regulates Roco2 kinase activity in vivo and that filamin lies downstream from Roco2 and controls pseudopod extension during chemotaxis and random cell motility. Therefore our study uncovered a new signaling pathway that involves Rab1A and controls the actin cytoskeleton and pseudopod extension, and thereby, cell polarity and motility. These findings also may have implications in the regulation of other Roco kinases, including possibly LRRK2, in metazoans.

Live imaging of mammalian embryonic development

SPIE Newsroom ◽  
2011 ◽  
Author(s):  
Kirill Larin ◽  
Irina V. Larina ◽  
Mary E. Dickinson
Keyword(s):  
Embryonic Development ◽  
Live Imaging ◽  
Mammalian Embryonic Development
Sign in / Sign up

Export Citation Format

Share Document