Denaturing Stop Buffer:

doi:10.1101/pdb.rec078725

20 kV 4H-SiC N-IGBTs

Materials Science Forum ◽

10.4028/www.scientific.net/msf.778-780.1030 ◽

2014 ◽

Vol 778-780 ◽

pp. 1030-1033 ◽

Cited By ~ 17

Author(s):

Sei Hyung Ryu ◽

Craig Capell ◽

Charlotte Jonas ◽

Michael J. O'Loughlin ◽

Jack Clayton ◽

...

Keyword(s):

Buffer Layer ◽

Leakage Current ◽

Supply Voltage ◽

Boost Converter ◽

Power Switching ◽

Switching Device ◽

Blocking Voltage ◽

Off Time ◽

Drift Layer ◽

Stop Buffer

A 1 cm x 1 cm 4H-SiC N-IGBT exhibited a blocking voltage of 20.7 kV with a leakage current of 140 μA, which represents the highest blocking voltage reported from a semiconductor power switching device to this date. The device used a 160 μm thick drift layer and a 1 μm thick Field-Stop buffer layer, and showed a VF of 6.4 V at an IC of 20 A, and a differential Ron,sp of 28 mΩ-cm2. Switching measurements with a supply voltage of 8 kV were performed, and a turn-off time of 1.1 μs and turn-off losses of 10.9 mJ were measured at 25°C, for a 8.4 mm x 8.4 mm device with 140 μm drift layer and 2 μm F-S buffer layer. The turn-off losses were reduced by approximately 50% by using a 5 μm F-S buffer layer. A 55 kW, 1.7 kV to 7 kV boost converter operating at 5 kHz was demonstrated using the 4H-SiC N-IGBT, and an efficiency value of 97.8% was reported.

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Rapid, high throughput library preparation of SARS-CoV2 using Illumina’s DNA Prep Library Preparation Kit v2

10.17504/protocols.io.b3vgqn3w ◽

2022 ◽

Author(s):

Jason Nguyen ◽

Rebecca Hickman ◽

Tracy Lee ◽

Natalie Prystajecky ◽

John Tyson

Keyword(s):

Whole Genome Sequencing ◽

Genome Sequencing ◽

High Throughput ◽

Illumina Miseq ◽

Whole Genome ◽

Library Preparation ◽

Dna Libraries ◽

Reaction Volumes ◽

Single Size ◽

Stop Buffer

This procedure provides instructions on how to prepare DNA libraries for whole genome sequencing on an Illumina MiSeq or NextSeq using Illumina’s DNA Prep Library Preparation Kit scaled to half reaction volumes with modifications to the post-PCR procedures; tagmentation stop buffer and associated washes are removed and libraries are pooled post PCR then a single size selection is performed. This protocol is used to sequence SARS-CoV-2 using the cDNA/PCR protocol: https://dx.doi.org/10.17504/protocols.io.b3viqn4e

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Stop buffer I

Cold Spring Harbor Protocols ◽

10.1101/pdb.rec11246 ◽

2009 ◽

Vol 2009 (12) ◽

pp. pdb.rec11246-pdb.rec11246

Keyword(s):

Stop Buffer

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Transcription stop buffer

Cold Spring Harbor Protocols ◽

10.1101/pdb.rec10897 ◽

2007 ◽

Vol 2007 (3) ◽

pp. pdb.rec10897

Keyword(s):

Stop Buffer

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Stop buffer (17-4)

Cold Spring Harbor Protocols ◽

10.1101/pdb.rec8829 ◽

2006 ◽

Vol 2006 (1) ◽

pp. pdb.rec8829

Keyword(s):

Stop Buffer

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Surfactants Attenuate Gas Embolism-induced Thrombin Production

Anesthesiology ◽

10.1097/00000542-200401000-00015 ◽

2004 ◽

Vol 100 (1) ◽

pp. 77-84 ◽

Cited By ~ 25

Author(s):

David M. Eckmann ◽

Scott L. Diamond

Keyword(s):

Shear Rate ◽

Blood Clot ◽

Specific Substrate ◽

Bonferroni Correction ◽

Gas Embolism ◽

Blood Samples ◽

Mean Values ◽

Surface Active ◽

Surface Active Compounds ◽

Stop Buffer

Background There are no pharmacologic strategies to prevent embolism bubble-induced blood clot formation. The authors conducted experiments to measure thrombin production in sheared whole blood in the presence and absence of bubbles and three surface-active compounds. Methods Blood samples were obtained from six volunteers seven times. The thrombin-specific substrate Boc-VPR-MCA was added to citrated blood diluted with HEPES-buffered saline. Experimental groups were as follows: sparging (air microbubble embolization) with surfactant present; sparging alone; surfactant alone; and neither surfactant nor sparging. The surfactants were Dow Corning Antifoam 1510US, Perftoran, and Pluronic F-127. Blood was sheared by a cone-plate viscometer at 100 and 500 s-1 for 5, 10, and 20 min at 37 degrees C, pipetted into excess stop buffer, and evaluated fluorimetrically. Mean values of fluorescence intensity +/- SDs for each group were compared using ANOVA. Differences were considered significant at P < 0.05 using the Bonferroni correction. Results For fixed shear rate, thrombin production increased 2.3- to 5.7-fold (P < 0.05) as shear duration lengthened. For fixed shear duration, thrombin production increased 1.9- to 3.9-fold (P < 0.05) with increasing shear rate. For fixed shear rate and duration, sparging increased thrombin production 2.1- to 3.7-fold (P < 0.05). Surfactant addition without sparging did not change thrombin production (P > 0.05). Surfactants attenuated thrombin production in sparged samples 31.8-70.9% (P < 0.05). Conclusions Thrombin production is shear rate and duration-dependent. Sparging increases thrombin production. Surfactants added before sparging attenuate thrombin production. Surfactants may have a clinical application to attenuate gas embolism-induced clotting.

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