scholarly journals Effects of Proteolysis on the Photochemical Activity and Polypeptide Composition of the Photosystem II Core Complex Isolated from Spinach Chloroplasts

1981 ◽  
Vol 68 (5) ◽  
pp. 1008-1013 ◽  
Author(s):  
Joseph A. Laszlo ◽  
Elizabeth L. Gross
Keyword(s):  
Photosystem Ii ◽  
Photochemical Activity ◽  
Polypeptide Composition ◽  
Core Complex ◽  
Spinach Chloroplasts

Polypeptide composition of a Photosystem II core complex. Presence of a herbicide-binding protein

1983 ◽  
Vol 724 (1) ◽  
pp. 142-150 ◽  
Author(s):  
K. Satoh ◽  
H.Y. Nakatani ◽  
K.E. Steinback ◽  
J. Watson ◽  
C.J. Arntzen
Keyword(s):  
Photosystem Ii ◽  
Binding Protein ◽  
Polypeptide Composition ◽  
Core Complex ◽  
Herbicide Binding

Photoactivation of the water-oxidizing complex of photosystem II core complex depleted of functional Mn

1991 ◽  
Vol 1060 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Noriaki Tamura ◽  
Hiroyuki Kamachi ◽  
Nobuyuki Hokari ◽  
Harutoshi Masumoto ◽  
Hiroshi Inoué
Keyword(s):  
Photosystem Ii ◽  
Core Complex ◽  
Water Oxidizing Complex

scholarly journals Crystallization of the Photosystem II core complex and its chlorophyll binding subunit CP43 from transplastomic plants of Nicotiana tabacum

2010 ◽  
Vol 106 (3) ◽  
pp. 221-226 ◽  
Author(s):  
Dario Piano ◽  
Sabah El Alaoui ◽  
Henryk J. Korza ◽  
Renata Filipek ◽  
Izabela Sabala ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Photosystem Ii ◽  
Core Complex ◽  
Transplastomic Plants ◽  
Binding Subunit

scholarly journals Subunit composition of CP43-less photosystem II complexes of Synechocystis sp. PCC 6803: implications for the assembly and repair of photosystem II

2012 ◽  
Vol 367 (1608) ◽  
pp. 3444-3454 ◽  
Author(s):  
M. Boehm ◽  
J. Yu ◽  
V. Reisinger ◽  
M. Beckova ◽  
L. A. Eichacker ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Gel Electrophoresis ◽  
Photochemical Activity ◽  
Subunit Composition ◽  
Null Mutant ◽  
Trap Potential ◽  
Native Gel ◽  
Oxygen Evolving ◽  
Pcc 6803

Photosystem II (PSII) mutants are useful experimental tools to trap potential intermediates involved in the assembly of the oxygen-evolving PSII complex. Here, we focus on the subunit composition of the RC47 assembly complex that accumulates in a psbC null mutant of the cyanobacterium Synechocystis sp. PCC 6803 unable to make the CP43 apopolypeptide. By using native gel electrophoresis, we showed that RC47 is heterogeneous and mainly found as a monomer of 220 kDa. RC47 complexes co-purify with small Cab-like proteins (ScpC and/or ScpD) and with Psb28 and its homologue Psb28-2. Analysis of isolated His-tagged RC47 indicated the presence of D1, D2, the CP47 apopolypeptide, plus nine of the 13 low-molecular-mass (LMM) subunits found in the PSII holoenzyme, including PsbL, PsbM and PsbT, which lie at the interface between the two momomers in the dimeric holoenzyme. Not detected were the LMM subunits (PsbK, PsbZ, Psb30 and PsbJ) located in the vicinity of CP43 in the holoenzyme. The photochemical activity of isolated RC47-His complexes, including the rate of reduction of P680 + , was similar to that of PSII complexes lacking the Mn 4 CaO 5 cluster. The implications of our results for the assembly and repair of PSII in vivo are discussed.

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