scholarly journals Broad HIV neutralization by a vaccine-induced cow antibody

2020 ◽  
Vol 76 (a1) ◽  
pp. a113-a113
Author(s):  
Robyn Stanfield
Keyword(s):  
Hiv Neutralization

Implications from the SIV model in understanding HIV neutralization

2008 ◽  
Vol 2 (1) ◽  
pp. 47-58 ◽  
Author(s):  
Anna Laurén ◽  
Eva Maria Fenyö
Keyword(s):  
Hiv Neutralization

scholarly journals Designed oligomers of cyanovirin-N show enhanced HIV neutralization

2011 ◽  
Vol 108 (34) ◽  
pp. 14079-14084 ◽  
Author(s):  
J. R. Keeffe ◽  
P. N. P. Gnanapragasam ◽  
S. K. Gillespie ◽  
J. Yong ◽  
P. J. Bjorkman ◽  
...  
Keyword(s):  
Hiv Neutralization

scholarly journals MPER-specific antibodies induce gp120 shedding and irreversibly neutralize HIV-1

2011 ◽  
Vol 208 (3) ◽  
pp. 439-454 ◽  
Author(s):  
Claudia R. Ruprecht ◽  
Anders Krarup ◽  
Lucy Reynell ◽  
Axel M. Mann ◽  
Oliver F. Brandenberg ◽  
...  
Keyword(s):  
Long Term Effects ◽  
Specific Antibodies ◽  
Molecular Events ◽  
Slow Kinetics ◽  
Cd4 Binding Site ◽  
Hiv Neutralization ◽  
Antibody Inhibition ◽  
Hiv 1 ◽  
Gp41 Envelope

Interference with virus entry is known to be the principle mechanism of HIV neutralization by antibodies, including 2F5 and 4E10, which bind to the membrane-proximal external region (MPER) of the gp41 envelope protein. However, to date, the precise molecular events underlying neutralization by MPER-specific antibodies remain incompletely understood. In this study, we investigated the capacity of these antibodies to irrevocably sterilize HIV virions. Long-term effects of antibodies on virions can differ, rendering neutralization either reversible or irreversible. MPER-specific antibodies irreversibly neutralize virions, and this capacity is associated with induction of gp120 shedding. Both processes have similar thermodynamic properties and slow kinetics requiring several hours. Antibodies directed to the CD4 binding site, V3 loop, and the MPER can induce gp120 shedding, and shedding activity is detected with high frequency in plasma from patients infected with divergent genetic HIV-1 subtypes. Importantly, as we show in this study, induction of gp120 shedding is closely associated with MPER antibody inhibition, constituting either a primary event leading to virion neutralization or representing an immediate consequence thereof, and thus needs to be factored into the mechanistic processes underlying their activity.

Detection of very early antibody to native HIV antigens by HIV neutralization and live-cell immunofluorescence assays

2006 ◽  
Vol 70 (2) ◽  
pp. 21-27
Author(s):  
J PAN ◽  
J CHEN ◽  
H LEE ◽  
G SAHU ◽  
J POAST ◽  
...  
Keyword(s):  
Live Cell ◽  
Hiv Neutralization

scholarly journals Automated image-based assay for evaluation of HIV neutralization and cell-to-cell fusion inhibition

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Enas Sheik-Khalil ◽  
Mark-Anthony Bray ◽  
Gülsen Özkaya Şahin ◽  
Gabriella Scarlatti ◽  
Marianne Jansson ◽  
...  
Keyword(s):  
Cell Fusion ◽  
Fusion Inhibition ◽  
Hiv Neutralization

Turning a corner on HIV neutralization?

2000 ◽  
Vol 2 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Jack H Nunberg ◽  
Kathryn E Follis ◽  
Meg Trahey ◽  
Rachel A LaCasse
Keyword(s):  
Hiv Neutralization

scholarly journals Preparation of functional IgY that potently neutralizes HIV-1 in TZM-bl Cell line

2021 ◽  
Author(s):  
Jinchen Wei ◽  
Yanqun Zhang ◽  
Yonglian Zeng ◽  
Yang Yang ◽  
Ronggan Liang ◽  
...  
Keyword(s):  
Cell Line ◽  
Neutralizing Antibodies ◽  
Evaluation System ◽  
Salting Out ◽  
Novel Technology ◽  
Public Health Challenges ◽  
Hiv Neutralization ◽  
Hiv 1

ABSTRACTAIDS caused by HIV is one of the most serious public health challenges in the world. As we all know, Antiretroviral therapy (ART) is the most effective way to treat AIDS so far, however, forthe reasons of drug resistance, side effects, compliance, economy, limited its using widely. On the other hand, AIDS cannot be completely cured by ART. While the characterization of bnAbs (broadly neutralizing antibodies) in potent HIV neutralization provides considerable insight into HIV curing, it also can be used for passive immunotherapy or combination with ART for HIV-1 treatment. Here we report a novel technology to produce an neutralized activity bnAbs named HIV-1-IgY, which was extracted from the immunized Chicken egg by pNL4-3 virus antigens, and further purified using Water dilution and Salting out method. The specificity, titer and neutralizing activity of HIV-1-IgY was analyzed by Western blotting, ELISA and TZM-bl cell line evaluation system respectively. The results showed that theHIV-1-IgY has high neutralized activity to HIV in vitro; nearly 90% of HIV-1 were neutralized at 1.89μM in TZM-blsystem, which indicated that IgY may be a source of antibodies for AIDS prevention and treatment. Despite its needs to further consider and evaluate neutralized activity in-vivo and the potential mechanisms, Our data showed that we obtained an HIV-1-IgY which could effectively neutralize HIV-l IIIB virus in vitro.

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