Point Spread Function Measurement of a Home-Made Structured Illumination Microscope using Non-Fluorescent Large Object Imaging

2019 ◽  
Author(s):  
Mahdieh Chartab Jabbari ◽  
Amir H. Baradaran Ghasemi ◽  
Mohammad Ghandi Monfared ◽  
Hamid Latifi
Keyword(s):  
Point Spread Function ◽  
Structured Illumination ◽  
Function Measurement ◽  
Large Object ◽  
Point Spread ◽  
Spread Function ◽  
Object Imaging

scholarly journals High-fidelity structured illumination microscopy by point-spread-function engineering

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Gang Wen ◽  
Simin Li ◽  
Linbo Wang ◽  
Xiaohu Chen ◽  
Zhenglong Sun ◽  
...  
Keyword(s):  
Point Spread Function ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
High Fidelity ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Imaging Tool ◽  
Point Spread ◽  
Spread Function ◽  
Point Spread Function Engineering

AbstractStructured illumination microscopy (SIM) has become a widely used tool for insight into biomedical challenges due to its rapid, long-term, and super-resolution (SR) imaging. However, artifacts that often appear in SIM images have long brought into question its fidelity, and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly seen artifacts without loss of fine structures and improve the axial sectioning for samples with strong background. In particular, HiFi-SIM is not sensitive to the commonly used PSF and reconstruction parameters; hence, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool.

scholarly journals Point Spread Function Measurement Based on Single-Pixel Imaging

2018 ◽  
Vol 10 (6) ◽  
pp. 1-15
Author(s):  
Hongzhi Jiang ◽  
Yangchenxu Liu ◽  
Xudong Li ◽  
Huijie Zhao ◽  
Feng Liu
Keyword(s):  
Point Spread Function ◽  
Function Measurement ◽  
Point Spread ◽  
Spread Function ◽  
Single Pixel

2D Sub-pixel Point Spread Function Measurement Using a Virtual Point-Like Source

2016 ◽  
Vol 121 (3) ◽  
pp. 391-402 ◽  
Author(s):  
Jurij Jemec ◽  
Franjo Pernuš ◽  
Boštjan Likar ◽  
Miran Bürmen
Keyword(s):  
Point Spread Function ◽  
Function Measurement ◽  
Point Spread ◽  
Spread Function ◽  
Virtual Point
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