Magnetic Epidermal Growth Factor Conjugate for Targeted Delivery to Grafted Tumor in Mouse Model

2013 ◽  
Vol 49 (1) ◽  
pp. 429-435 ◽  
Author(s):  
Boris. P. Nikolaev ◽  
Yaroslav Yu Marchenko ◽  
Liudmila Yu Yakovleva ◽  
Tatiana M. Zimina ◽  
Alexei V. Soloviev ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Mouse Model ◽  
Targeted Delivery ◽  
Epidermal Growth

scholarly journals Ectodomain of Coxsackievirus and Adenovirus Receptor Genetically Fused to Epidermal Growth Factor Mediates Adenovirus Targeting to Epidermal Growth Factor Receptor-Positive Cells

2000 ◽  
Vol 74 (15) ◽  
pp. 6875-6884 ◽  
Author(s):  
Igor Dmitriev ◽  
Elena Kashentseva ◽  
Buck E. Rogers ◽  
Victor Krasnykh ◽  
David T. Curiel
Keyword(s):  
Growth Factor ◽  
Gene Transfer ◽  
Epidermal Growth Factor ◽  
Targeted Delivery ◽  
Attachment Site ◽  
Soluble Form ◽  
Target Cells ◽  
Epidermal Growth ◽  
Coxsackievirus And Adenovirus Receptor ◽  
Adenovirus Receptor

ABSTRACT Human adenovirus (Ad) is extensively used for a variety of gene therapy applications. However, the utility of Ad vectors is limited due to the low efficiency of Ad-mediated gene transfer to target cells expressing marginal levels of the Ad fiber receptor. Therefore, the present generation of Ad vectors could potentially be improved by modification of Ad tropism to target the virus to specific organs and tissues. The fact that coxsackievirus and adenovirus receptor (CAR) does not play any role in virus internalization, but functions merely as the virus attachment site, suggests that the extracellular part of CAR might be utilized to block the receptor recognition site on the Ad fiber knob domain. We proposed to design bispecific fusion proteins formed by a recombinant soluble form of truncated CAR (sCAR) and a targeting ligand. In this study, we derived sCAR genetically fused with human epidermal growth factor (EGF) and investigated its ability to target Ad infection to the EGF receptor (EGFR) overexpressed on cancer cell lines. We have demonstrated that sCAR-EGF protein is capable of binding to Ad virions and directing them to EGFR, thereby achieving targeted delivery of reporter gene. These results show that sCAR-EGF protein possesses the ability to effectively retarget Ad via a non-CAR pathway, with enhancement of gene transfer efficiency.

scholarly journals Epidermal Growth Factor Receptor: Key to Selective Intracellular Delivery

2020 ◽  
Vol 85 (9) ◽  
pp. 967-993 ◽  
Author(s):  
A. A. Rosenkranz ◽  
T. A. Slastnikova
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cancer Cells ◽  
Targeted Delivery ◽  
Growth Factor Receptor ◽  
Chemotherapeutic Agents ◽  
Egfr Signaling Pathway ◽  
Egfr Activation ◽  
Epidermal Growth

Abstract Epidermal growth factor receptor (EGFR) is an integral surface protein mediating cellular response to a number of growth factors. Its overexpression and increased activation due to mutations is one of the most common traits of many types of cancer. Development and clinical use of the agents, which block EGFR activation, became a prime example of the personalized targeted medicine. However, despite the obvious success in this area, cancer cure remains unattainable in most cases. Because of that, as well as the result of the search for possible ways to overcome the difficulties of treatment, a huge number of new treatment methods relying on the use of EGFR overexpression and its changes to destroy cancer cells. Modern data on the structure, functioning, and intracellular transport of EGFR, its natural ligands, as well as signaling cascades triggered by the EGFR activation, peculiarities of the EGFR expression and activation in oncological disorders, as well as applied therapeutic approaches aimed at blocking EGFR signaling pathway are summarized and analyzed in this review. Approaches to the targeted delivery of various chemotherapeutic agents, radionuclides, immunotoxins, photosensitizers, as well as the prospects for gene therapy aimed at cancer cells with EGFR overexpression are reviewed in detail. It should be noted that increasing attention is being paid nowadays to the development of multifunctional systems, either carrying several different active agents, or possessing several environment-dependent transport functions. Potentials of the systems based on receptor-mediated endocytosis of EGFR and their possible advantages and limitations are discussed.

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