Reliability of cytological evaluation of mast cells from bronchoalveolar lavage fluid in horses: Intraobserver agreement and mast cell identification

2019 ◽  
Vol 32 (S11) ◽  
pp. 47-52 ◽  
Author(s):  
S. Hansen ◽  
J. Fjeldborg ◽  
A. J. Hansen ◽  
K. E. Baptiste
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Intraobserver Agreement ◽  
Cell Identification ◽  
Cytological Evaluation

Cytological evaluation of bronchoalveolar lavage fluid in Osmanabadi goats

2021 ◽  
Vol 27 (2) ◽  
pp. 305-308
Author(s):  
R.R. Deshpande ◽  
P.D. Gadhave ◽  
K.H. Ingole ◽  
V.D. Thorat ◽  
D.P. Kadam ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Cytological Evaluation

Adenosine induces histamine release from human bronchoalveolar lavage mast cells

1999 ◽  
Vol 96 (4) ◽  
pp. 349-355 ◽  
Author(s):  
P. FORSYTHE ◽  
L. P. A. MCGARVEY ◽  
L. G. HEANEY ◽  
J. MACMAHON ◽  
M. ENNIS
Keyword(s):  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Histamine Release ◽  
Adenosine Receptor ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Maximal Response ◽  
Adenosine Receptor Agonists ◽  
Receptor Agonists ◽  
Endogenous Adenosine

Previous studies have shown that in vitroadenosine enhances histamine release from activated human lung mast cells obtained by enzymic dispersion of lung parenchyma. However, adenosine alone has no effect on histamine release from these cells. Given the evidence for direct activation of mast cells after endobronchial challenge with adenosine and previous studies indicating that mast cells obtained at bronchoalveolar lavage are a better model for asthma studies than those obtained by enzymic dispersion of lung tissue, the histamine-releasing effect of adenosine was examined on lavage mast cells. Bronchoalveolar lavage fluid was obtained from patients attending hospital for routine bronchoscopy (n = 54). Lavage cells were challenged with adenosine or adenosine receptor agonists (20 min, 37 °C) and histamine release determined using an automated fluorometric assay. Endogenous adenosine levels were also measured in lavage fluid (n = 9) via an HPLC method. Adenosine alone caused histamine release from lavage mast cells in 37 of 54 patients with a maximal histamine release of 20.56±2.52% (range 5.2–61%). The adenosine receptor agonists (R)-N6-(2-phenylisopropyl)adenosine, 5'-N-ethylcarboxamidoadenosine and CGS21680 also induced histamine release from lavage mast cells. Preincubation of lavage mast cells with the adenosine receptor antagonist xanthine amine congener caused significant inhibition of the response to adenosine (P = 0.007). There was an inverse correlation between endogenous adenosine levels in the lavage fluid and the maximal response to in vitro adenosine challenge of the lavage cells. The findings of the present study indicate a means by which adenosine challenge of the airways can induce bronchoconstriction and support a role for adenosine in the pathophysiology of asthma. The results also suggest that cells obtained from bronchoalveolar lavage fluid may provide the ideal model for the testing of novel, adenosine receptor, targeted therapies for asthma.

Identification of mast cells in bronchoalveolar lavage fluid.

Allergy ◽  
1991 ◽  
Vol 46 (3) ◽  
pp. 222-227 ◽  
Author(s):  
A. Xaubet ◽  
J. A. Moisés ◽  
C. Agustí ◽  
J. A. Martos ◽  
C. Picado
Keyword(s):  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid

Pretreatment with Methylprednisolone in Coronary Artery Bypass Grafting Influences the Levels of Histamine and Tryptase in Serum but Not in Bronchoalveolar Lavage Fluid

1994 ◽  
Vol 86 (1) ◽  
pp. 49-53 ◽  
Author(s):  
F. J. van Overveld ◽  
R. F. De Jongh ◽  
P. G. Jorens ◽  
P. Walter ◽  
L. Bossaert ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Coronary Artery Bypass Grafting ◽  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Coronary Artery Bypass ◽  
Extracorporeal Circulation ◽  
Bronchoalveolar Lavage Fluid ◽  
Artery Bypass ◽  
Lavage Fluid ◽  
Bypass Grafting

1. The presence of histamine and tryptase in serum during and after coronary artery bypass grafting may be an indication of the induction of inflammation. 2. One group of patients received no glucocorticoids and a second group received methylprednisolone before extracorporeal circulation. In the steroid group no effects were seen on the basal levels of histamine (2.84 + 0.12 ng/ml) and tryptase (030 + 0.05 ng/ml) during and after surgery. In the other group two peak levels of histamine were observed: one at 10 min after starting extracorporeal circulation (4.19 +1.79 ng/ml) and another at 4h after surgery (8.26 +4.85 ng/ml). In this group tryptase was only elevated during the period of extracorporeal circulation (1.54+ 0.16 ng/ml). 3. There were no differences between the two groups in complement activation. C3a levels rose to 170 +8% and 180 +10% of the initial value in the steroid and non-steroid group, respectively. 4. It was concluded that during surgery mast cells were activated, but since tryptase levels decreased in the post-operative period, the second increase in the histamine level can be explained by activation of basophils or by an unknown mechanism for the release of histamine but not tryptase by mast cells. 5. In the bronchoalveolar lavage fluid the levels of histamine and tryptase showed no differences between the two groups of patients, but histamine was enhanced compared with normal levels.

scholarly journals Hyaluronic acid in bronchoalveolar lavage fluid in patients with sarcoidosis: relationship to lavage mast cells.

Thorax ◽  
1987 ◽  
Vol 42 (12) ◽  
pp. 933-938 ◽  
Author(s):  
L Bjermer ◽  
A Engstrom-Laurent ◽  
M Thunell ◽  
R Hallgren
Keyword(s):  
Hyaluronic Acid ◽  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid

INCREASED NUMBERS OF MAST CELLS IN BRONCHOALVEOLAR LAVAGE FLUID IN ASTHMATIC DOGS

1985 ◽  
Vol 63 (Supplement) ◽  
pp. A538
Author(s):  
C. A. Hirshman ◽  
D. R. Austin ◽  
J. M. Hanifin ◽  
W. Hulbert
Keyword(s):  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid
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