scholarly journals Hyaluronic acid in bronchoalveolar lavage fluid in patients with sarcoidosis: relationship to lavage mast cells.

Thorax ◽  
1987 ◽  
Vol 42 (12) ◽  
pp. 933-938 ◽  
Author(s):  
L Bjermer ◽  
A Engstrom-Laurent ◽  
M Thunell ◽  
R Hallgren
Keyword(s):  
Hyaluronic Acid ◽  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid

Adenosine induces histamine release from human bronchoalveolar lavage mast cells

1999 ◽  
Vol 96 (4) ◽  
pp. 349-355 ◽  
Author(s):  
P. FORSYTHE ◽  
L. P. A. MCGARVEY ◽  
L. G. HEANEY ◽  
J. MACMAHON ◽  
M. ENNIS
Keyword(s):  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Histamine Release ◽  
Adenosine Receptor ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Maximal Response ◽  
Adenosine Receptor Agonists ◽  
Receptor Agonists ◽  
Endogenous Adenosine

Previous studies have shown that in vitroadenosine enhances histamine release from activated human lung mast cells obtained by enzymic dispersion of lung parenchyma. However, adenosine alone has no effect on histamine release from these cells. Given the evidence for direct activation of mast cells after endobronchial challenge with adenosine and previous studies indicating that mast cells obtained at bronchoalveolar lavage are a better model for asthma studies than those obtained by enzymic dispersion of lung tissue, the histamine-releasing effect of adenosine was examined on lavage mast cells. Bronchoalveolar lavage fluid was obtained from patients attending hospital for routine bronchoscopy (n = 54). Lavage cells were challenged with adenosine or adenosine receptor agonists (20 min, 37 °C) and histamine release determined using an automated fluorometric assay. Endogenous adenosine levels were also measured in lavage fluid (n = 9) via an HPLC method. Adenosine alone caused histamine release from lavage mast cells in 37 of 54 patients with a maximal histamine release of 20.56±2.52% (range 5.2–61%). The adenosine receptor agonists (R)-N6-(2-phenylisopropyl)adenosine, 5'-N-ethylcarboxamidoadenosine and CGS21680 also induced histamine release from lavage mast cells. Preincubation of lavage mast cells with the adenosine receptor antagonist xanthine amine congener caused significant inhibition of the response to adenosine (P = 0.007). There was an inverse correlation between endogenous adenosine levels in the lavage fluid and the maximal response to in vitro adenosine challenge of the lavage cells. The findings of the present study indicate a means by which adenosine challenge of the airways can induce bronchoconstriction and support a role for adenosine in the pathophysiology of asthma. The results also suggest that cells obtained from bronchoalveolar lavage fluid may provide the ideal model for the testing of novel, adenosine receptor, targeted therapies for asthma.

Identification of mast cells in bronchoalveolar lavage fluid.

Allergy ◽  
1991 ◽  
Vol 46 (3) ◽  
pp. 222-227 ◽  
Author(s):  
A. Xaubet ◽  
J. A. Moisés ◽  
C. Agustí ◽  
J. A. Martos ◽  
C. Picado
Keyword(s):  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid

Clinical utility of hyaluronic acid values in serum and bronchoalveolar lavage fluid as tumor marker for bronchogenic carcinoma

1995 ◽  
Vol 10 (3) ◽  
pp. 149-155 ◽  
Author(s):  
J.R. Hernández Hernández ◽  
J.M. García García ◽  
M.A. Martínez Muñíz ◽  
M.T. Allende Monclus ◽  
A. Ruibal Morell
Keyword(s):  
Hyaluronic Acid ◽  
Bronchoalveolar Lavage ◽  
Tumor Marker ◽  
Cell Carcinoma ◽  
Bronchogenic Carcinoma ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Small Cell ◽  
Limited Disease ◽  
Benign Diseases

Various authors have proposed the use of hyaluronic acid (HA) as a tumor marker. In order to analyze its usefulness as a marker in bronchogenic carcinoma, the most common carcinoma in men, we determined the HA values in serum and bronchoalveolar lavage fluid (BAL). We performed prospective studies on two groups of patients: 81 diagnosed as having bronchial carcinoma and 34 with benign respiratory diseases. HA values were higher in patients with cancer than in those with benign diseases (serum: 79.8 ng/ml vs 63.7 ng/ml; BAL: 927 ng/mg vs 522 ng/mg). Also, the percentage of patients with levels exceeding the established cutoff was greater in the group with cancer than in the group with benign diseases (serum: 24.6 vs 17.6; BAL: 25.3 vs 3). Statistically significant differences in these percentages were found in BAL (p<0.01). Patients with extended small cell carcinoma had higher HA values (p=0.04) than those with limited disease, and the percentage of patients with abnormal HA values was larger in the group with extended disease than in the group with limited disease (p=0.004). The serial determinations of HA values in serum reflected the clinical evolution after treatment in 73% of the small cell carcinomas. Most of the patients with benign diseases whose HA values exceeded the cutoff level suffered from acute infectious dis-eases. Once these cases were excluded, the specificity of HA value determination in the diagnosis of carcinoma was very high (serum 96%, BAL 100%). The determination of HA levels in serum or BAL did not have any prognostic value in this study. We conclude that the HA levels in serum and BAL could be of interest as a tumor marker, especially in patients with small cell carcinoma.

Pretreatment with Methylprednisolone in Coronary Artery Bypass Grafting Influences the Levels of Histamine and Tryptase in Serum but Not in Bronchoalveolar Lavage Fluid

1994 ◽  
Vol 86 (1) ◽  
pp. 49-53 ◽  
Author(s):  
F. J. van Overveld ◽  
R. F. De Jongh ◽  
P. G. Jorens ◽  
P. Walter ◽  
L. Bossaert ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Coronary Artery Bypass Grafting ◽  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Coronary Artery Bypass ◽  
Extracorporeal Circulation ◽  
Bronchoalveolar Lavage Fluid ◽  
Artery Bypass ◽  
Lavage Fluid ◽  
Bypass Grafting

1. The presence of histamine and tryptase in serum during and after coronary artery bypass grafting may be an indication of the induction of inflammation. 2. One group of patients received no glucocorticoids and a second group received methylprednisolone before extracorporeal circulation. In the steroid group no effects were seen on the basal levels of histamine (2.84 + 0.12 ng/ml) and tryptase (030 + 0.05 ng/ml) during and after surgery. In the other group two peak levels of histamine were observed: one at 10 min after starting extracorporeal circulation (4.19 +1.79 ng/ml) and another at 4h after surgery (8.26 +4.85 ng/ml). In this group tryptase was only elevated during the period of extracorporeal circulation (1.54+ 0.16 ng/ml). 3. There were no differences between the two groups in complement activation. C3a levels rose to 170 +8% and 180 +10% of the initial value in the steroid and non-steroid group, respectively. 4. It was concluded that during surgery mast cells were activated, but since tryptase levels decreased in the post-operative period, the second increase in the histamine level can be explained by activation of basophils or by an unknown mechanism for the release of histamine but not tryptase by mast cells. 5. In the bronchoalveolar lavage fluid the levels of histamine and tryptase showed no differences between the two groups of patients, but histamine was enhanced compared with normal levels.

INCREASED NUMBERS OF MAST CELLS IN BRONCHOALVEOLAR LAVAGE FLUID IN ASTHMATIC DOGS

1985 ◽  
Vol 63 (Supplement) ◽  
pp. A538
Author(s):  
C. A. Hirshman ◽  
D. R. Austin ◽  
J. M. Hanifin ◽  
W. Hulbert
Keyword(s):  
Mast Cells ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid
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