A rediscovered corpuscle in the fresh preparation of blood

2009 ◽  
Vol 60 (1) ◽  
pp. 12-21 ◽  
Author(s):  
ROBIN FÅHRAEUS
Keyword(s):  
Fresh Preparation

scholarly journals Ligusticum sinense Nanoemulsion Gel as Potential Repellent against Aedes aegypti, Anopheles minimus, and Culex quinquefasciatus (Diptera: Culicidae)

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 596
Author(s):  
Anuluck Junkum ◽  
Wanchai Maleewong ◽  
Atiporn Saeung ◽  
Danita Champakaew ◽  
Arpaporn Chansang ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Disease Transmission ◽  
Skin Irritation ◽  
Mosquito Vectors ◽  
Plant Family ◽  
Anopheles Minimus ◽  
Human Volunteers ◽  
Nanoemulsion Gel ◽  
Fresh Preparation

Ligusticum sinense Oliv. cv. is a species of Umbelliferae (Apiaceae), a large plant family in the order Apiales. In this study, L. sinense hexane extract nanoemulsion gel (LHE-NEG) was investigated for mosquito repellency and compared to the standard chemical, N,N-diethyl-3-methylbenzamide (DEET), with the goal of developing a natural alternative to synthetic repellents in protecting against mosquito vectors. The results demonstrated that LHE-NEG afforded remarkable repellency against Aedes aegypti, Anopheles minimus, and Culex quinquefasciatus, with median protection times (MPTs) of 5.5 (4.5–6.0), 11.5 (8.5–12.5), and 11.25 (8.5–12.5) h, respectively, which was comparable to those of DEET-nanoemulsion gel (DEET-NEG: 8.5 (7.0–9.0), 12.0 (10.0–12.5), and 12.5 (10.0–13.5) h, respectively). Evaluation of skin irritation in 30 human volunteers revealed no potential irritant from LHE-NEG. The physical and biological stability of LHE-NEG were determined after being kept under heating/cooling cycle conditions. The stored samples of LHE-NEG exhibited some changes in appearance and differing degrees of repellency between those kept for 3 and 6 heating/cooling cycles, thus providing slightly shorter MPTs of 4.25 (4.0–4.5) and 3.25 (2.5–3.5) h, respectively, when compared to those of 5.0 (4.5–6.0) h in fresh preparation. These findings encourage commercially developed LHE-based products as an alternative to conventional synthetic repellents in preventing mosquito bites and helping to interrupt mosquito-borne disease transmission.

Intranodular Treatment with BCG of Cutaneous and Subcutaneous Metastases of Malignant Melanoma.

1974 ◽  
Vol 60 (4) ◽  
pp. 345-352 ◽  
Author(s):  
Gian Paolo Balzarini ◽  
Natale Cascinelli ◽  
Vincenzo Fontana ◽  
Umberto Veronesi
Keyword(s):  
Side Effects ◽  
Malignant Melanoma ◽  
Therapeutic Action ◽  
Caseous Necrosis ◽  
Subcutaneous Metastases ◽  
Short Time ◽  
Fresh Preparation

A fresh preparation of BCG (Istituto Vaccinogeno Antitubercolare, Milano) having a bacterial charge of 0.5 mg/ml was injected intranodularly in cutaneous and subcutaneous metastases of malignant melanoma of 22 patients. A total of 85 nodules were treated. Caseous necrosis was observed in 17 patients equal to 68 nodules. The side-effects were modest and cleared up in a short time. The results obtained were localized at the treated nodules, without giving rise to a therapeutic action on those not injcted. The evolution of the disease was not modified.

scholarly journals Effect of Fresh Preparation and Industrial Processing on Stability of Potato Phytochemicals

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
A Furrer ◽  
D Cladis ◽  
A Kurilich ◽  
G Hawkins ◽  
R Manoharan ◽  
...  
Keyword(s):  
Industrial Processing ◽  
Fresh Preparation

The Morphological and Anatomical Studies of The Aerial Parts of Abroma augusta L. from Semarang

2021 ◽  
Vol 6 (2) ◽  
pp. 222-234
Author(s):  
Rita Ariyana Nur Khasanah ◽  
Niken Kusumarini
Keyword(s):  
Glandular Trichomes ◽  
Plant Identification ◽  
Simple Method ◽  
Anatomical Studies ◽  
Aerial Parts ◽  
Palisade And Spongy Parenchyma ◽  
Conservation Effort ◽  
Binocular Microscope ◽  
Black Seeds ◽  
Fresh Preparation

Abroma augusta L. known as Devil’s cotton belongs to Malvaceae. The exploratory study aimed to study the morphological and anatomical characteristics of the aerial parts of A. augusta L. from Semarang. The transverse section of the aerial parts was made by a simple method (fresh preparation) and then observed under a binocular microscope with an optilab. All characteristics were observed and then compared with the references. The collected data were analyzed descriptively and quantitatively. In summary, the results showed that A. augusta L. was an evergreen shrub (small tree) with orthotropic and plagiotropic branches and polymorphous leaves. The inflorenscence was found in the terminal and axillar plagiotropic branching with bisex, actinomorphic, and pentamerous flowers. The fruit was unique (obconical capsule with a rounded base and truncate-tip with 5 angled wings) including cotton fibers and numerous black seeds. The petiole was composed of epidermis, collenchyma, cortical parenchyma, sclerenchyma, vascular bundle, mucilaginous ducts, and pith. The dorsiventral leaf was composed of upper and lower epidermis, palisade, and spongy parenchyma. The stomata type was ranunculaceous (anomocytic) while the guard cell was kidney-shaped. The stomata density on the abaxial leaf was higher than that of the adaxial leaf. The stellate and unicellular non-glandular trichomes, and capitate glandular trichomes were found abundantly on the petiole and leaf blade. These morphological and anatomical studies are important to support the identification as a part of the conservation effort of the plant. Further studies are recommended to investigate the root morphology and anatomy and also biochemical characteristics of each part of the plant in order to obtain  complete plant identification.

Note on the Occurrence of a Herpetomonad in Glossina morsitans

1924 ◽  
Vol 15 (2) ◽  
pp. 185-186 ◽  
Author(s):  
Ll. Lloyd
Keyword(s):  
Salivary Glands ◽  
Northern Nigeria ◽  
Glossina Morsitans ◽  
Free Swimming ◽  
The Third ◽  
Fresh Preparation

In a recent number of this Bulletin, Lloyd and Johnson (1) attempted to describe and illustrate all the known types of flagellate infections encountered in Glossina. Since this account was written a flagellate, which it is believed has not previously been described, has been encountered in three individuals of G. morsitans at Sherifuri, Northern Nigeria. In the first two cases the worker at the microscope was new to the infections of tsetse and did not notice anything peculiar about the parasite in the proboscides that were being examined. The appearance of the parasites in the stained film caused one slide to be retained with a note, but it was at that time regarded as an aberrant T. vivax infection. In the second instance T. vivax was present, and the unusual forms accompanying it were remarked upon, but unfortunately the slide was not retained. In the third instance it was noticed in the fresh preparation that none of the parasites were fixed to the labrum, as are the vast majority of those in T. vivax infections, nor were they clumped as in this species and in T. congolense. Each individual was free-swimming and many were escaping from the labial cavity, the power of translatory movement being great. The hypo-pharynx was not invaded. The remainder of the fly was then examined and the mid gut was found to be heavily infected, while the salivary glands were negative.

scholarly journals Spinal Reflexes of the Dogfish, Scyllium canicula

1936 ◽  
Vol 13 (2) ◽  
pp. 210-218
Author(s):  
J. GRAY ◽  
A. SAND
Keyword(s):  
Spinal Cord ◽  
Rhythmic Activity ◽  
Spinal Reflexes ◽  
Striking Resemblance ◽  
Reflex Responses ◽  
Peripheral Stimulation ◽  
Fresh Preparation

1. The persistent undulatory rhythm of the spinal dogfish can be reversibly augmented, accelerated and arrested by peripheral stimulation. 2. These reflexes are a property of the whole spinal cord, and are not localised in any particular level. 3. In a state of fatigue the reflex responses become modified, in particular a stimulus which, in the fresh preparation, caused excitation, inhibits the rhythm in the fatigued fish. The significance of this is discussed. 4. The behaviour of the fatigued intact dogfish approximates to that of the spinal fish. A striking resemblance in the response to inhibitory stimuli is described, and the thesis is developed that the persistent rhythm of the spinal fish is the expression of a basic rhythmic activity of the spinal cord.

Kandungan Escherichia coli dan Higiene Sanitasi Pengolahan pada Beberapa Pedagang Minuman Es Kelapa di Kecamatan Oebobo Kota Kupang

2020 ◽  
Vol 2 (1) ◽  
pp. 29-39
Author(s):  
Damelia Mandala ◽  
Yuliana Radja Riwu ◽  
Sarci M. Toy ◽  
Muntasir Basri
Keyword(s):  
Escherichia Coli ◽  
Raw Materials ◽  
Total Sample ◽  
Collection Method ◽  
Food Ingredients ◽  
Food Borne ◽  
The Republic ◽  
Escherichia Coli Bacteria ◽  
Ministerial Decree ◽  
Fresh Preparation

Young coconut ice drink is one of the snacks known for its delicious taste and its practical and fresh preparation in Kupang. Young coconut ice drinks have the potential to be contaminated so that they can cause food-borne illnesses (food borne diseases). One of the causes is the Escherichia coli bacterium, which occurs because of the hygienic treatment of foods that do not meet the health requirements. This type of research is descriptive by examining the contents of Escherichia coli and the hygiene description of young coconut drinks suitable for street food snacks in the Oebobo district from 17 to 22 July 2019. The research involves grocers and coconut ice-creams, as well as raw materials for the production of water in all young coconut ice drink producers in the Oebobo district. The number of young coconut ice drink producers in the Oebobo district is 10, so the total sample was used in this study. The data collection method uses observational sheets. The results of this study indicate that there are 6 out of 10 positive samples containing Escherichia coli bacteria, based on the Ministerial Decree of the Republic of Indonesia No. 492/Menkes/Per/IV/2010. The presence of Escherichia coli is said to be contaminated. There are 4 out of 10 manufacturers that meet the health requirements for food handling, equipment, water, food ingredients, additives and service, outlets and dealer centers, based on the Health Minister Decree No. 942 / MENKES / SK / VII / 2003 of the Republic of Indonesia in Terms of hygiene food for snacks.

scholarly journals High engraftment capacity of frozen ready-to-use human fecal microbiota transplants assessed in germ-free mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Magali Berland ◽  
Julie Cadiou ◽  
Florence Levenez ◽  
Nathalie Galleron ◽  
Benoît Quinquis ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
Fecal Microbiota Transplant ◽  
Shotgun Metagenomics ◽  
Germ Free ◽  
Important Group ◽  
Freeze Dried ◽  
The Family ◽  
Fresh Preparation

AbstractThe number of indications for fecal microbiota transplantation is expected to rise, thus increasing the needs for production of readily available frozen or freeze-dried transplants. Using shotgun metagenomics, we investigated the capacity of two novel human fecal microbiota transplants prepared in maltodextrin-trehalose solutions (abbreviated MD and TR for maltodextrin:trehalose, 3:1, w/w, and trehalose:maltodextrin 3:1, w/w, respectively), to colonize a germ-free born mouse model. Gavage with frozen-thawed MD or TR suspensions gave the taxonomic profiles of mouse feces that best resembled those obtained with the fresh inoculum (Spearman correlations based on relative abundances of metagenomic species around 0.80 and 0.75 for MD and TR respectively), while engraftment capacity of defrosted NaCl transplants most diverged (Spearman correlations around 0.63). Engraftment of members of the family Lachnospiraceae and Ruminoccocaceae was the most challenging in all groups of mice, being improved with MD and TR transplants compared to NaCl, but still lower than with the fresh preparation. Improvement of engraftment of this important group in maintaining health represents a challenge that could benefit from further research on fecal microbiota transplant manufacturing.

scholarly journals Purification and properties of bovine caeruloplasmin

1981 ◽  
Vol 199 (3) ◽  
pp. 667-673 ◽  
Author(s):  
L Calabrese ◽  
F Malatesta ◽  
D Barra
Keyword(s):  
Copper Content ◽  
General Structure ◽  
Molecular Weight Determination ◽  
Copper Determination ◽  
Purification And Properties ◽  
Different Types ◽  
Novel Method ◽  
Fresh Preparation

A novel method is reported for isolation of bovine caeruloplasmin from plasma; it involves a rapid and mild procedure, namely two column chromatographies with stepwise elution and one (NH4)2SO4 precipitation, and results in a proteolytically undegraded homogeneous protein. The general structure of the protein, as evaluated by molecular-weight determination and amino acid composition, is very similar to that established for human and rat caeruloplasmin. Copper determination and e.p.r. spectral analysis on the native and NO-treated protein gave a metal-to-protein stoichiometry of six atoms of copper per molecule. Three copper atoms were detectable by e.p.r., with Type 2/Type 1 ratio = 1 : 3 in most samples. The protein is very sensitive to storage and/or handling. A component was isolated from aged samples, which was found to contain approximately four copper atoms per 125000 daltons, two of which were detectable by e.p.r. with the characters of Type 2 copper. However, the same component was found to be present, although to a lesser extent, in the fresh preparation and does not seem to be related to proteolytic degradation. This component has no oxidase activity. On the basis of these results it is suggested that caeruloplasmin molecules are intrinsically heterogeneous with respect to both copper content and copper type, and this can explain the intriguing stoichiometry regarding the different types of copper centres.

Fresh Preparation of Subretinal Fluid Aspirations in Coats' Disease

1985 ◽  
Vol 100 (2) ◽  
pp. 327-328 ◽  
Author(s):  
Barrett G. Haik ◽  
June Koizumi ◽  
Mary E. Smith ◽  
Robert M. Ellsworth
Keyword(s):  
Subretinal Fluid ◽  
Coats Disease ◽  
Fresh Preparation
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