Retinoic Acid Treatment Induces Cell Death and the Protein Expression of Retinoic Acid Receptor beta in the Mesenchymal Cells of Mouse Facial Primordia in Vitro. (RA/facial mesenchymal cells/chondrogenesis/cell death/RAR b and g protein expression/ micromass culture)

1994 ◽  
Vol 36 (3) ◽  
pp. 281-288 ◽  
Author(s):  
Jun Motoyama ◽  
Keiko Taki ◽  
Noriko Osumi-Yamashita ◽  
Kazuhiro Eto
Keyword(s):  
Cell Death ◽  
Retinoic Acid ◽  
Protein Expression ◽  
Acid Treatment ◽  
Retinoic Acid Receptor ◽  
Mesenchymal Cells ◽  
Micromass Culture ◽  
Retinoic Acid Treatment ◽  
Receptor Beta

scholarly journals Inducible Gene Trapping with Drug-Selectable Markers and Cre/loxP To Identify Developmentally Regulated Genes

2004 ◽  
Vol 24 (22) ◽  
pp. 9930-9941 ◽  
Author(s):  
You-Tzung Chen ◽  
Pentao Liu ◽  
Allan Bradley
Keyword(s):  
Retinoic Acid ◽  
Acid Treatment ◽  
Gene Trap ◽  
In Vitro Differentiation ◽  
Retinoic Acid Treatment ◽  
Developmentally Regulated ◽  
Gene Trapping ◽  
Gene Traps ◽  
Inducible Gene

ABSTRACT Gene trapping in mouse embryonic stem cells is an important genetic approach that allows simultaneous mutation of genes and generation of corresponding mutant mice. We designed a selection scheme with drug selection markers and Cre/loxP technology which allows screening of gene trap events that responded to a signaling molecule in a 96-well format. Nine hundred twenty gene trap clones were assayed, and 258 were classified as gene traps induced by in vitro differentiation. Sixty-five of the in vitro differentiation-inducible gene traps were also responsive to retinoic acid treatment. In vivo analysis revealed that 85% of the retinoic acid-inducible gene traps trapped developmentally regulated genes, consistent with the observation that genes induced by retinoic acid treatment are likely to be developmentally regulated. Our results demonstrate that the inducible gene trapping system described here can be used to enrich in vitro for traps in genes of interest. Furthermore, we demonstrate that the cre reporter is extremely sensitive and can be used to explore chromosomal regions that are not detectable with neo as a selection cassette.

Short-Term Retinoic Acid Treatment Increases In Vivo, but Decreases In Vitro, Epidermal Transglutaminase-K Enzyme Activity and Immunoreactivity

1992 ◽  
Vol 99 (3) ◽  
pp. 283-288 ◽  
Author(s):  
Christopher E M Griffiths ◽  
Dean S Rosenthal ◽  
Ambati P Reddy ◽  
James T Elder ◽  
Anders. Astrom ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Retinoic Acid ◽  
Acid Treatment ◽  
Short Term ◽  
Retinoic Acid Treatment ◽  
Epidermal Transglutaminase

scholarly journals A novel model of in vitro osteocytogenesis induced by retinoic acid treatment

2012 ◽  
Vol 24 ◽  
pp. 403-425 ◽  
Author(s):  
D Mattinzoli ◽  
◽  
P Messa ◽  
A Corbelli ◽  
M Ikehata ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Acid Treatment ◽  
Retinoic Acid Treatment ◽  
Novel Model
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