Genetic variability in environmental isolates of Legionella pneumophila from Comunidad Valenciana (Spain)

2006 ◽  
Vol 8 (6) ◽  
pp. 1056-1063 ◽  
Author(s):  
Mireia Coscolla ◽  
Maria Jose Gosalbes ◽  
Vicente Catalan ◽  
Fernando Gonzalez-Candelas
Keyword(s):  
Genetic Variability ◽  
Legionella Pneumophila ◽  
Environmental Isolates

scholarly journals Australian Isolates of Legionella longbeachae Are Not a Clonal Population

1999 ◽  
Vol 37 (10) ◽  
pp. 3249-3254 ◽  
Author(s):  
J. C. Montanaro-Punzengruber ◽  
L. Hicks ◽  
W. Meyer ◽  
G. L. Gilbert
Keyword(s):  
Genetic Variability ◽  
Legionella Pneumophila ◽  
Gel Electrophoresis ◽  
Type Strain ◽  
Pulsed Field Gel Electrophoresis ◽  
Epidemiological Investigation ◽  
Environmental Isolates ◽  
Clonal Population ◽  
Pulsed Field ◽  
Major Cluster

Legionella longbeachae is almost as frequent a cause of legionellosis in Australia as Legionella pneumophila, but epidemiological investigation of possible environmental sources and clinical cases has been limited by the lack of a discriminatory subtyping method. The purpose of this study was to examine the genetic variability among Australian isolates of L. longbeachaeserogroup 1. Pulsed-field gel electrophoresis (PFGE) ofSfiI fragments revealed three distinct pulsotypes among 57 clinical and 11 environmental isolates and the ATCC control strains of L. longbeachae serogroups 1 and 2. Each pulsotype differed by four bands, corresponding to <65% similarity. A clonal subgroup within each pulsotype was characterized by >88% similarity. The largest major cluster was pulsotype A, which included 43 clinical isolates and 9 environmental isolates and was divided into five subgroups. Pulsotypes B and C comprised smaller numbers of clinical and environmental isolates, which could each be further divided into three subgroups. The ATCC type strain of L. longbeachae serogroup 1 was classified as pulsotype B, subtype B3, while the ATCC type strain of L. longbeachae serogroup 2 was identified as a different pulsotype, LL2. SfiI macrorestriction analysis followed by PFGE showed that the AustralianL. longbeachae strains are not a single clonal population as previously reported.

scholarly journals Lessons From an Outbreak of Legionnaires’ Disease on a Hematology-Oncology Unit

2016 ◽  
Vol 38 (3) ◽  
pp. 306-313 ◽  
Author(s):  
Louise K. Francois Watkins ◽  
Karrie-Ann E. Toews ◽  
Aaron M. Harris ◽  
Sherri Davidson ◽  
Stephanie Ayers-Millsap ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Potable Water ◽  
Single Case ◽  
Healthcare Providers ◽  
Water System ◽  
Positive Sequence ◽  
Environmental Isolates ◽  
Legionnaires Disease ◽  
Oncology Unit ◽  
Healthcare Associated

OBJECTIVESTo define the scope of an outbreak of Legionnaires’ disease (LD), to identify the source, and to stop transmission.DESIGN AND SETTINGEpidemiologic investigation of an LD outbreak among patients and a visitor exposed to a newly constructed hematology-oncology unit.METHODSAn LD case was defined as radiographically confirmed pneumonia in a person with positive urinary antigen testing and/or respiratory culture forLegionellaand exposure to the hematology-oncology unit after February 20, 2014. Cases were classified as definitely or probably healthcare-associated based on whether they were exposed to the unit for all or part of the incubation period (2–10 days). We conducted an environmental assessment and collected water samples for culture. Clinical and environmental isolates were compared by monoclonal antibody (MAb) and sequence-based typing.RESULTSOver a 12-week period, 10 cases were identified, including 6 definite and 4 probable cases. Environmental sampling revealedLegionella pneumophilaserogroup 1 (Lp1) in the potable water at 9 of 10 unit sites (90%), including all patient rooms tested. The 3 clinical isolates were identical to environmental isolates from the unit (MAb2-positive, sequence type ST36). No cases occurred with exposure after the implementation of water restrictions followed by point-of-use filters.CONCLUSIONSContamination of the unit’s potable water system with Lp1 strain ST36 was the likely source of this outbreak. Healthcare providers should routinely test patients who develop pneumonia at least 2 days after hospital admission for LD. A single case of LD that is definitely healthcare associated should prompt a full investigation.Infect Control Hosp Epidemiol2017;38:306–313

scholarly journals Complete Genome Sequences of Six Legionella pneumophila Isolates from Two Collocated Outbreaks of Legionnaires’ Disease in 2005 and 2008 in Sarpsborg/Fredrikstad, Norway

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Marius Dybwad ◽  
Tone Aarskaug ◽  
Else-Marie Fykse ◽  
Elisabeth Henie Madslien ◽  
Janet Martha Blatny
Keyword(s):  
Comparative Genomics ◽  
Genetic Relationship ◽  
Legionella Pneumophila ◽  
Complete Genome ◽  
Sequence Similarity ◽  
Secretion Systems ◽  
Environmental Isolates ◽  
Genome Sequences ◽  
High Sequence Similarity ◽  
Legionnaires Disease

Here, we report the complete genome sequences of Legionella pneumophila isolates from two collocated outbreaks of Legionnaires’ disease in 2005 and 2008 in Sarpsborg/Fredrikstad, Norway. One clinical and two environmental isolates were sequenced from each outbreak. The genome of all six isolates consisted of a 3.36 Mb-chromosome, while the 2005 genomes featured an additional 68 kb-episome sharing high sequence similarity with the L. pneumophila Lens plasmid. All six genomes contained multiple mobile genetic elements including novel combinations of type-IVA secretion systems. A comparative genomics study will be launched to resolve the genetic relationship between the L. pneumophila isolates.

scholarly journals Molecular Epidemiology ofLegionella pneumophilaInfection at a Canadian Tertiary Care Institution

1994 ◽  
Vol 5 (4) ◽  
pp. 157-162 ◽  
Author(s):  
LE Nicolle ◽  
H Bialkowska-Hobrzanska ◽  
B Dyck ◽  
L Sekla ◽  
S Parker
Keyword(s):  
Monoclonal Antibody ◽  
Molecular Epidemiology ◽  
Legionella Pneumophila ◽  
Tertiary Care ◽  
Environmental Isolates ◽  
Tertiary Care Centre ◽  
Clonal Type ◽  
Serotype 1 ◽  
Single Clone ◽  
Hospital Acquired

Objective: To characterize the molecular epidemiology ofLegionellaspecies infection at one Canadian tertiary care centre.Design: Twenty-eight clinical isolates and 12 environmental isolates obtained over a six-year period were analyzed by restriction fragment length polymorphism (rflp) of chromosomaldna. Isolates included 15 from 12 patients with hospital acquired illness and 13 from nine patients with community acquired infection.Results: One nosocomial strain wasLegionella micdadeiand one community strain wasLegionella pneumophilaserotype 6; all others wereL pneumophilaserotype 1.rflptyping revealed one clone for all cases of a 1985 single-ward outbreak and five of six nonoutbreakL pneumophilanosocomial cases. Anrflppattern identical or highly related to that of the nosocomial clonal type was noted among nine of 12L pneumophilaserotype 1 community isolates. The remaining three isolates had two relatedrflppatterns distinct from the institutional strain. The nosocomial and community strains were isolated from multiple institutional water samples in the institution. For the environmental isolates, monoclonal antibody typing was more discriminating thanrflptyping: seven monoclonal antibody subtypes were distinguished among 12 environmental isolates comprising three distinctrflppatterns.Conclusions: Despite multipleL pneumophilaserotype 1 strains isolated in the authors’ institutional water, a single clone ofL pneumophilaproduced most disease. Community acquired disease was caused by a wider variety of strains.

Differential Proteome Between Patient-Related and Non-related Environmental Isolates of Legionella pneumophila

2017 ◽  
Vol 74 (3) ◽  
pp. 344-355
Author(s):  
Sara Quero ◽  
Marian García-Núñez ◽  
Noemí Párraga-Niño ◽  
M. Luisa Pedro-Botet ◽  
Lourdes Mateu ◽  
...  
Keyword(s):  
Legionella Pneumophila ◽  
Environmental Isolates

scholarly journals Nosocomial legionella pneumonia: demonstration of potable water as the source of infection

1988 ◽  
Vol 101 (3) ◽  
pp. 647-654 ◽  
Author(s):  
B. Ruf ◽  
D. Schürmann ◽  
I. Horbrach ◽  
K. Seodel ◽  
H. D. Pohle
Keyword(s):  
Monoclonal Antibodies ◽  
Water Supply ◽  
Legionella Pneumophila ◽  
Water Samples ◽  
Potable Water ◽  
Supply System ◽  
University Hospital ◽  
Environmental Isolates ◽  
Legionella Pneumonia ◽  
Source Of Infection

SUMMARYFrom January 1983 until December 1985, 35 cases of sporadic nosocomial legionella pneumonia, all caused byLegionella pneumophila, were diagnosed in a university hospital.L. pneumophilaserogroup (SG) 1 was cultured from 12 of the 35 cases and compared to correspondingL. pneumophilaSG 1 isolates from water outlets in the patients' immediate environment by subtyping with monoclonal antibodies. The corresponding environmental isolates were identical to 9 out of 12 (75%) of those from the cases. However, even in the remaining three cases identical subtypes were found distributed throughout the hospital water supply. From the hospital water supply four different subtypes ofL. pneumophilaSG 1 were isolated, three of which were implicated in legionella pneumonia. Of 453 water samples taken during the study 298 (65.8%) were positive for legionellae. Species ofLegionellaother thanL. pneumophilahave not been isolated. This may explain the exclusiveness ofL.pneumophilaas the legionella pneumonia-causing agent. Our results suggest that the water supply system was the source of infection.

Sign in / Sign up

Export Citation Format

Share Document