AN ASSOCIATION OF PHYTOCHROME WITH THE CHLOROPLAST ENVELOPE MEMBRANES OF SPINACIA OLERACEA L.: A PRELIMINARY OBSERVATION

1983 ◽  
Vol 95 (2) ◽  
pp. 175-178 ◽  
Author(s):  
JANET R. HILTON
Keyword(s):  
Spinacia Oleracea ◽  
Preliminary Observation ◽  
Chloroplast Envelope ◽  
Spinacia Oleracea L ◽  
Envelope Membranes

Distribution of Chlorophylls, Carotenoids and Quinones in Chloroplasts of Higher Plants

1983 ◽  
Vol 38 (11-12) ◽  
pp. 996-1002 ◽  
Author(s):  
K. H. Grumbach
Keyword(s):  
Raphanus Sativus ◽  
Spinacia Oleracea ◽  
Higher Plants ◽  
Chloroplast Envelope ◽  
Spinacia Oleracea L ◽  
Raphanus Sativus L ◽  
Chlorophyll A And B ◽  
Isolated Chloroplasts ◽  
Β Carotene

Leaves, cotyledons, isolated chloroplasts and subplastid fractions (thylakoids and envelopes) of radish (Raphanus sativus L. cv. Saxa) and spinach (Spinacia oleracea L. cv. M atador) were assayed for their pigment and quinone content and com position. Virtually all the chlorophylls, carotenoids and quinones were contained in the thylakoids. Envelopes prepared by the method described contained very low amounts o f chlorophyll a and b, violaxanthin and neoxanthin, but no β-carotene, lutein, zeaxanthin and antheraxanthin. Among the quinones trace amounts of plastoquinone and a-tocopherol but no plastohydroquinone, a-tocoquinone and phylloquinone were detected. Presented data may be taken as evidence that in vivo the chloroplast envelope is not a location site of carotenoids and quinones as generally accepted. Possible im plications for the biosyntheses of quinones and pigments are discussed

Versuche zur präparativen Isolierung pflanzlicher Zellorganellen mittels der trägerfreien, kontinuierlichen Ablenkungselektrophorese

1964 ◽  
Vol 19 (11) ◽  
pp. 1072-1075 ◽  
Author(s):  
K. Hannig ◽  
W. Klofat ◽  
H. Endres
Keyword(s):  
Spinacia Oleracea ◽  
Taraxacum Officinale ◽  
Wird Eine ◽  
Spinacia Oleracea L ◽  
Helianthus Annus

Es wird eine präparative Methode zur Isolierung pflanzlicher Zellbestandteile mittels der trägerfreien, kontinuierlichen Ablenkungselektrophorese nach HANNIG 1, 2 kurz skizziert. Als Versuchsmaterial dienen Blätter von Spinat (Spinacia oleracea L.), Sonnenblumen (Helianthus annus L.) und Löwenzahn (Taraxacum officinale Web.).Die Verteilungskurven werden durch Extinktionsmessung der einzelnen Fraktionen in den Auffanggläschen erhalten. Die Definition der Teilchen erfolgt vorläufig morphologisch durch Anfärben und lichtmikroskopische Prüfung als auch durch elektronenmikroskopische Kontrolle nach vorheriger Präparation nach dem „negativ staining“ Verfahren 3,4 oder Kontrastierung mit Phosphorwolframsäure.Bei der Trennung werden Zellkerne und Zellkernfragmente, ganze Chloroplasten, „Mitochondrien“ und plasmatische Strukturen erhalten.

scholarly journals A review on the genetic resources, domestication and breeding history of spinach (Spinacia oleracea L.)

Euphytica ◽  
2020 ◽  
Vol 216 (3) ◽  
Author(s):  
Arnau Ribera ◽  
Yuling Bai ◽  
Anne-Marie A. Wolters ◽  
Rob van Treuren ◽  
Chris Kik
Keyword(s):  
Genetic Resources ◽  
Spinacia Oleracea ◽  
Spinacia Oleracea L ◽  
History Of ◽  
Breeding History

Nitrate metabolism in relation to the aspartate-type C-4 pathway of photosynthesis in Eleusine coracana

1974 ◽  
Vol 52 (12) ◽  
pp. 2599-2605 ◽  
Author(s):  
C. K. M. Rathnam ◽  
V. S. R. Das
Keyword(s):  
Glutamate Dehydrogenase ◽  
Eleusine Coracana ◽  
Bundle Sheath ◽  
Chloroplast Envelope ◽  
Mesophyll Cells ◽  
Bundle Sheath Cells ◽  
Nitrate Metabolism ◽  
Type C ◽  
Envelope Membranes ◽  
Sheath Cells

The intercellular and intracellular distributions of nitrate assimilating enzymes were studied. Nitrate reductase was found to be localized on the chloroplast envelope membranes. The chloroplastic NADPH – glutamate dehydrogenase was concentrated in the mesophyll cells. The extrachloroplastic NADH – glutamate dehydrogenase was localized in the bundle sheath cells. Glutamate synthesized in the mesophyll chloroplasts was interpreted to be utilized exclusively in the synthesis of aspartate, while in the bundle sheath cells it was thought to be consumed in other cellular metabolic processes. Based on the results, a scheme is proposed to account for the nitrate metabolism in the leaves of Eleusine coracana Gaertn. in relation to its aspartate-type C-4 pathway of photosynthesis.

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