scholarly journals Low copy number plasmid vectors for gene cloning and for monitoring gene expression

1986 ◽  
Vol 37 (2) ◽  
pp. 193-197 ◽  
Author(s):  
Hilde Reuse ◽  
Eliette Touati ◽  
Philippe Glaser ◽  
Antoine Danchin
Keyword(s):  
Gene Expression ◽  
Gene Cloning ◽  
Copy Number ◽  
Plasmid Vectors ◽  
Copy Number Plasmid ◽  
Low Copy Number ◽  
Monitoring Gene Expression

Development of Small High-Copy-Number Plasmid Vectors for Gene Expression in Caulobacter crescentus

Plasmid ◽  
2001 ◽  
Vol 46 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Elizabeth Umelo-Njaka ◽  
John F. Nomellini ◽  
Harry Yim ◽  
John Smit
Keyword(s):  
Gene Expression ◽  
Copy Number ◽  
Caulobacter Crescentus ◽  
High Copy Number ◽  
Plasmid Vectors ◽  
Copy Number Plasmid ◽  
High Copy Number Plasmid

Versatile low-copy-number plasmid vectors for cloning in Escherichia coli

Gene ◽  
1982 ◽  
Vol 18 (3) ◽  
pp. 335-341 ◽  
Author(s):  
Neil G. Stoker ◽  
Neu F. Fairweathe ◽  
Brian G. Spratt
Keyword(s):  
Escherichia Coli ◽  
Copy Number ◽  
Plasmid Vectors ◽  
Copy Number Plasmid ◽  
Low Copy Number

High-copy-number and low-copy-number plasmid vectors for lacZα-complementation and chloramphenicol- or kanamycin-resistance selection

Gene ◽  
1987 ◽  
Vol 61 (1) ◽  
pp. 63-74 ◽  
Author(s):  
Sunao Takeshita ◽  
Masahiro Sato ◽  
Mari Toba ◽  
Wakako Masahashi ◽  
Tamotsu Hashimoto-Gotoh
Keyword(s):  
Copy Number ◽  
Kanamycin Resistance ◽  
High Copy Number ◽  
Plasmid Vectors ◽  
Resistance Selection ◽  
Copy Number Plasmid ◽  
Low Copy Number

scholarly journals Targeted Isolation of a Designated Region of the Bacillus subtilis Genome by Recombinational Transfer

2004 ◽  
Vol 70 (4) ◽  
pp. 2508-2513 ◽  
Author(s):  
Satoshi Tomita ◽  
Kenji Tsuge ◽  
Yo Kikuchi ◽  
Mitsuhiro Itaya
Keyword(s):  
Bacillus Subtilis ◽  
Copy Number ◽  
Positional Cloning ◽  
Deletion Mutant ◽  
Essential Gene ◽  
Transfer System ◽  
Copy Number Plasmid ◽  
Low Copy Number ◽  
Subtilis Genome

ABSTRACT A method for positional cloning of the Bacillus subtilis genome was developed. The method requires a set of two small DNA fragments that flank the region to be copied. A 38-kb segment that carries genes ppsABCDE encoding five enzymes for antibiotic plipastatin synthesis and another genome locus as large as 100 kb including one essential gene were examined for positional cloning. The positional cloning vector for ppsABCDE was constructed using a B. subtilis low-copy-number plasmid that faithfully copied the precise length of the 38-kb DNA in vivo via the recombinational transfer system of this bacterium. Structure of the copied DNA was confirmed by restriction enzyme analyses. Furthermore, the unaltered structure of the 38-kb DNA was demonstrated by complementation of a ppsABCDE deletion mutant.

scholarly journals A vector for studying plasmid stability functions in Streptomyces

1988 ◽  
Vol 51 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Kevin Kendall ◽  
John Cullum
Keyword(s):  
Copy Number ◽  
Stability Region ◽  
Selectable Marker ◽  
Plasmid Stability ◽  
Stability Function ◽  
Melanin Production ◽  
Stability Functions ◽  
Tyrosinase Gene ◽  
Copy Number Plasmid ◽  
Low Copy Number

SummaryWe constructed a cloning vector (pMT603) based on the low copy number plasmid SCP2*. pMT6O3 is unstable because it lacks the SCP2* stability region and carries the selectable marker thiostrepton-resistance and a tyrosinase gene which results in melanin production. This allows easy testing of plasmid stability and we demonstrated its usefulness by cloning a plasmid stability function.

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