ANTIGEN FROM COLON OF GERMFREE RATS AND ANTIBODIES IN HUMAN ULCERATIVE COLITIS*

2006 ◽  
Vol 124 (1) ◽  
pp. 377-394 ◽  
Author(s):  
Peter Perlmann ◽  
Sten Hammarström ◽  
Rutger Lagercrantz ◽  
Bengt E. Gustafsson
Keyword(s):  
Ulcerative Colitis ◽  
Germfree Rats

scholarly journals IMMUNOLOGICAL STUDIES IN ULCERATIVE COLITIS

1965 ◽  
Vol 122 (6) ◽  
pp. 1075-1086 ◽  
Author(s):  
Sten Hammarström ◽  
Rutger Lagercrantz ◽  
Peter Perlmann ◽  
Bengt E. Gustafsson
Keyword(s):  
Ulcerative Colitis ◽  
Blood Group ◽  
Hemagglutination Inhibition ◽  
Fluorescent Antibody ◽  
Slight Reduction ◽  
Blood Group Antigens ◽  
Antibody Staining ◽  
Human Sera ◽  
Group B ◽  
Germfree Rats

Sera from patients with ulcerative colitis contain antibodies which hemagglutinate sheep red cells, sensitized with phenol-water extracts from. colon, cecum, or feces of germfree rats. Minor concentrations of such antibodies are also present in a certain fraction of normal human sera. Hemagglutination and hemagglutination inhibition experiments with human erythrocytes and with the rat extracts showed that the latter contained an antigen similar to human blood group A antigen. In contrast, a blood group B-like antigen could not be detected in these extracts. However, experiments with eel serum indicated that these extracts also contained an antigen similar to the H antigen of the human ABO system. Absorption of ulcerative colitis sera with human A1 erythrocytes but not that with B or O erythrocytes gave, in a few cases, a slight reduction of the hemagglutinating titers against rat cecum-sensitized sheep erythrocytes. In contrast, this treatment considerably reduced such titers when found in sera from healthy persons or from patients with unrelated diseases. It could be concluded that the rat extracts also contained a "colon" antigen, detected with antibodies, present at elevated titers, in the sera of ulcerative colitis patients, but not in those of the controls. This colon antigen is immunologically distinct from the blood group antigens studied. Hemagglutination inhibition experiments indicated that A, H and colon antigen were widely distributed throughout the gastrointestinal tract of the germfree rats. The colon antigen was found to be enriched in the extracts from colon, cecum, and feces. Fluorescent antibody staining provided evidence that both the colon antigen and the A antigen were present in similar sites of the colon and cecum mucosa, particularly in goblet cells of the crypts, and in the mucus.

scholarly journals AUTOANTIBODIES TO COLON IN GERMFREE RATS MONOCONTAMINATED WITH CLOSTRIDIUM DIFFICILE

1969 ◽  
Vol 129 (4) ◽  
pp. 747-756 ◽  
Author(s):  
Sten Hammarström ◽  
Peter Perlmann ◽  
Bengt E. Gustafsson ◽  
Rutger Lagercrantz
Keyword(s):  
Ulcerative Colitis ◽  
Clostridium Difficile ◽  
Bacterial Degradation ◽  
Rat Colon ◽  
Bacterial Antigen ◽  
Sprague Dawley ◽  
Autoantibody Production ◽  
Passive Hemagglutination ◽  
Rabbit Colon ◽  
Germfree Rats

Germfree rats monocontaminated with the anaerobic microorganisms Clostridium difficile or another Clostridium species (strain G 62) produce auto-antibodies to colon antigen. The antigen can be extracted with phenol water from the feces of germfree rats. Antibodies, demonstrable by means of passive hemagglutination of antigen sensitized sheep erythrocytes appear after monocontamination for 35 days or longer. The indirect immunofluorescence techniques, applied to sections of germfree rat colon, gave positive mucosal staining. The staining was similar to that obtained with sera from patients with ulcerative colitis or from rats immunized with rabbit colon. No antibodies were found in the sera of germfree rats, germfree rats monocontaminated with various other bacteria, conventional rats of germfree origin, or conventional Sprague-Dawley rats. Although the anti-colon antibodies of the Clostridium infected rats reacted with the same feces extract as the antibodies of ulcerative colitis patients or of rabbit colon immunized rats, their specificity was different. While the latter cross-react with polysaccharide from E. coli O14, those from the Clostridium-infected exgermfree rats did not. Rats monocontaminated with Cl. difficile also developed antibodies to this organism, but no cross-reaction between Cl. difficile antigen and colon antigen could be demonstrated. This speaks against breakage of tolerance by cross-reacting bacterial antigen as the cause of autoimmunity in these rats. Other possible mechanisms for autoantibody production in this model are immunogenic alteration of gastrointestinal mucins by bacterial degradation, adjuvant effects of bacterial products, or both.

49. The Reaction of Antibodies in Human Ulcerative Colitis with Antigen from Colon of Germfree Rats and from E. Coli

1965 ◽  
Vol 54 (s159) ◽  
pp. 108-109
Author(s):  
R. LAGERCRANTZ ◽  
P. PERLMANN ◽  
S. HAMMARSTRÖM ◽  
B. E. GUSTAFSSON
Keyword(s):  
Ulcerative Colitis ◽  
E Coli ◽  
Germfree Rats

Histological and macroscopic changes in the pelvic pouch: long-term follow up after restorative proctocolectomy for ulcerative colitis (UC)

2001 ◽  
Vol 3 (1) ◽  
pp. 28-32 ◽  
Author(s):  
J. Tiainen ◽  
M. Matikainen ◽  
P. Aitola ◽  
K-M. Hiltunen ◽  
J. Mattila
Keyword(s):  
Ulcerative Colitis ◽  
Restorative Proctocolectomy ◽  
Pelvic Pouch ◽  
Long Term Follow Up

Mannan-binding lectin gene polymorphisms in ulcerative colitis and Crohn's disease

2001 ◽  
Vol 120 (5) ◽  
pp. A459-A459
Author(s):  
A RECTOR ◽  
P LEMEY ◽  
W LAFFUT ◽  
E KEYAERTS ◽  
F STRUYF ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Gene Polymorphisms ◽  
Lectin Gene ◽  
Mannan Binding Lectin ◽  
Binding Lectin

Small area variations and socio-demographic correlates for the incidence of Crohn s disease and ulcerative colitis

2001 ◽  
Vol 120 (5) ◽  
pp. A458-A458
Author(s):  
J BLANCHARD ◽  
A WAJDA ◽  
P RAWSTHORNE ◽  
C BERNSTEIN
Keyword(s):  
Ulcerative Colitis ◽  
Small Area ◽  
Demographic Correlates

Phase II study of safety, efficacy and pharmacokinetics of BXT-51072 in patients with mild-moderately active ulcerative colitis

2001 ◽  
Vol 120 (5) ◽  
pp. A280-A280
Author(s):  
S HANAUER ◽  
P MINER ◽  
A KESHAVARZIAN ◽  
E MORRIS ◽  
B SALZBERG ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Phase Ii ◽  
Phase Ii Study ◽  
Active Ulcerative Colitis

Deletion mapping on chromosome 6 in colorectal cancer associated with ulcerative colitis

2001 ◽  
Vol 120 (5) ◽  
pp. A121-A122
Author(s):  
T EZAKI ◽  
M WATANABE ◽  
S FUNAKOSHI ◽  
M NAGANUMA ◽  
T AZUMA ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Ulcerative Colitis ◽  
Deletion Mapping ◽  
Chromosome 6
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