SIZE AND SHAPE OF MACROMOLECULES IN PLASMA EXPANDERS

1956 ◽  
Vol 8 (1) ◽  
pp. 93-109 ◽  
Author(s):  
H. Campbell
Author(s):  
H.J.G. Gundersen

Previously, all stereological estimation of particle number and sizes were based on models and notoriously gave biased results, were very inefficient to use and difficult to justify. For all references to old methods and a direct comparison with unbiased methods see recent reviews.The publication in 1984 of the DISECTOR, the first unbiased stereological probe for sampling and counting 3—D objects irrespective of their size and shape, signalled the new era in stereology — and give rise to a number of remarkably simple and efficient techniques based on its distinct property: It is the only known way to obtain an unbiased sample of 3-D objects (cells, organelles, etc). The principle is simple: within a 2-D unbiased frame count or sample only cells which are not hit by a parallel plane at a known, small distance h.The area of the frame and h must be known, which might sometimes in itself be a problem, albeit usually a small one. A more severe problem may arise because these constants are known at the scale of the fixed, embedded and sectioned tissue which is often shrunken considerably.


Author(s):  
C J R Sheppard

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.


1984 ◽  
Vol 45 (C9) ◽  
pp. C9-29-C9-37
Author(s):  
Vu Thien Binh ◽  
M. Drechsler
Keyword(s):  

2020 ◽  
Vol 78 (4) ◽  
pp. 479-486
Author(s):  
Marcela Tatiana Fernandes Beserra ◽  
◽  
Ricardo Tadeu Lopes ◽  
Davi Ferreira de Oliveira ◽  
Claudio Carvalho Conti ◽  
...  

2017 ◽  
Vol 14 (2-3) ◽  
pp. 155-167
Author(s):  
Penny Brown

This paper considers the merit of manga versions of Shakespeare's Hamlet and Cervantes' Don Quijote de la Mancha which employ the impressionistic techniques of the Japanese comic format to create new, dynamic texts. Such multimodal texts demand different verbal and visual skills to decode the synergy between word and image and elements like the page layout, the size and shape of images and speech balloons and the style of lettering. Far from debasing the cultural authority of the originals by blurring the boundaries between high and popular culture, these versions can be seen as an act of salvage of the original texts from the perceived difficulties of challenging language and content, reinvigorating them with a vibrant immediacy. By making demands on the imagination and intellect in exciting ways, they may also salvage the act of reading itself by encouraging a young or reluctant readership, as well as the already enthusiastic, to explore new ways of engaging with a text.


Sign in / Sign up

Export Citation Format

Share Document