scholarly journals Mapping of the occurrence of grapevine viruses: GFLV and ArMV in vineyards of the grapevine breeding station Polešovice

2007 ◽  
Vol 55 (5) ◽  
pp. 117-122
Author(s):  
Pavel Pavloušek
Keyword(s):  
Czech Republic ◽  
Mosaic Virus ◽  
Elisa Test ◽  
Grapevine Fanleaf Virus ◽  
Virus Diseases ◽  
Arabis Mosaic Virus ◽  
Good Basis ◽  
Worldwide Distribution ◽  
Breeding Station ◽  
Positive Results

Due to their worldwide distribution and also detrimental potential virus diseases of grapevine are economically very important. In grapevine plants Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV) is one of the most harmful virus pathogens. The aim of this study was evaluate the occurrence of GFLV and ArMV in vineyards of the Grapevine Breeding Station Polešovice (Czech Republic) using the ELISA test and visual symptoms. In every year from 2001–2004 altogether 225 grapevine plants were tested for the occurrence of GFLV and ArMV. In case of GFLV positive results were obtained in 43 plants, i. e. in 19.11% of all samples. In case of ArMV there were 51 positive tests, i.e. 22.66%. Our results demonstrated a occurrence of GFLV and ArMV in propagation vineyards, which was comparable with results obtained abroad. It was also demonstrated that a professional visual selection could provide a good basis for the identification of virus diseases and their subsequent tests.

scholarly journals Suitability of the date of grapevine tissue sampling for tests of GFLV and ArMV presence using ELISA method

2007 ◽  
Vol 55 (5) ◽  
pp. 223-228
Author(s):  
Pavel Pavloušek
Keyword(s):  
Mosaic Virus ◽  
Growth Stages ◽  
Tissue Sampling ◽  
Grapevine Fanleaf Virus ◽  
Elisa Method ◽  
Arabis Mosaic Virus ◽  
Young Leaves ◽  
Beginning Of Flowering ◽  
Positive Results ◽  
Intensive Growth

Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV) are two important agents from the Nepoviruses genus. At present, serodiagnostic method is one of routine methods of virus pathogens testing also in grapevines. The aim of this study was to determine a suitable date of grapevine tissue sampling using the ELISA method and to follow visible symptoms of GFVL and ArMV infestation of vines. Positive results of tests for the occurrence of GFLV were obtained in the following growth stages: B (intensive growth – growth apex), C (beginning of flowering – young leaves) and D (beginning of flowering – inflorescence). Samples evaluated by ELISA method as positive for ArMV were found out in growth stages B (intensive growth – growth apex) and C (beginning of flowering – inflorescence). In all other stages the results of performed tests were negative. It is concluded that the stage B (intensive growth – growth apex) seems to be the most suitable for sampling of grapevine tissue.

Arabis mosaic virus. [Distribution map].

2010 ◽  
Author(s):  
Keyword(s):  
South Africa ◽  
South Carolina ◽  
Czech Republic ◽  
Northern Ireland ◽  
Mosaic Virus ◽  
Distribution Map ◽  
Arabis Mosaic Virus ◽  
Virus Distribution ◽  
Distribution In Europe ◽  
Xiphinema Diversicaudatum

Abstract A new ditribution map is provided for Arabis mosaic virus (ArMV; Comoviridae: Nepovirus). It has a wide host range. Information is given on the geographical distribution in Europe (Austria, Belarus, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Finland, France, Germany, Hungary, Ireland, Italy, Latvia, Lithuania, Luxembourg, Moldova, Netherlands, Norway, Poland, Romania, Russia, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, UK, England, Wales, Northern Ireland, Scotland and Ukraine), Asia (China, India, Himachal Pradesh, Iran, Israel, Japan, Honshu, Kazakhstan, Lebanon, Syria and Turkey), Africa (South Africa), North America (Canada, British Columbia, Nova Scotia, Ontario, Quebec, Mexico, USA, Connecticut, Florida, Michigan, Minnesota, Missouri, Nebraska and South Carolina), South America (Chile) and Oceania (Australia, Tasmania, Victoria and New Zealand). The main vector of ArMV is the nematode Xiphinema diversicaudatum. Other dorylaimid nematodes may also be vectors. ArMV is also seed-transmitted.

scholarly journals Effect of fungicide farmayod on agrotechnical and technological indicators of grapevine, on viral diseases and oidium

2021 ◽  
Vol 273 ◽  
pp. 01020
Author(s):  
Nadezda Sirotkina
Keyword(s):  
Mosaic Virus ◽  
Drug Concentration ◽  
Viral Diseases ◽  
Cabernet Sauvignon ◽  
Virus Concentration ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Drug Concentrations ◽  
Leaf Virus ◽  
Oidium Tuckeri

The paper presents the study on the effect of Farmayod’s GR (100 g/l of iodine) spraying on vineyards of Cabernet Sauvignon and Baklanovsky varieties on the degree of viral and oidium prevalence as well as on agrobiological and technological indicators. According to the aggregate agrobiological and technological indicators, the best results on Cabernet Sauvignon variety were obtained when the drug was used at a concentration of 0.06 %. On the Baklanovsky variety the best indicators were obtained at a drug concentration of 0.04%. Testing of plant samples for the presence of Grapevine fan leaf virus, Arabis mosaic virus and Oidium tuckeri showed that after two years of applying the drug, the prevalence of infected plants (P, %) with Grapevine fanleaf virus on the Cabernet Sauvignon cultivar varied from 0% (fungicide concentration 0.04 and 0.05 %) to 0.8 % (0.06 %) and 2.65 % (control). For Baklanovsky variety: Grapevine fanleaf virus - concentration 0.04 % - 1.8; 0.05 % - 0.4; 0.06 % - 2.0; control - 2.65 %. Arabis mosaic virus – 0; 0; 3.0; 12.1 %, respectively. Oidium tuckeri was 0 % in all variants with any drug concentrations. Control variant and later 80 % for 29.09.

scholarly journals Genetic Structure and Molecular Variability of Grapevine Fanleaf Virus in Sicily

Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 496
Author(s):  
Stefano Panno ◽  
Andrea Giovanni Caruso ◽  
Sofia Bertacca ◽  
Antonino Pisciotta ◽  
Rosario Di Lorenzo ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Phylogenetic Analyses ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Preliminary Screening ◽  
Molecular Variability ◽  
Italian Regions ◽  
Almost All ◽  
Positive Results ◽  
Das Elisa

Grapevine fanleaf virus (GFLV) is one of the main causes of grapevine fanleaf degeneration disease (GFDD) and is present in almost all areas where grapevine is cultivated. In this work, we ascertained the presence and spread of GFLV in different commercial vineyards in four Sicilian provinces (Italy), and its genetic structure and molecular variability were studied. In detail, a total of 617 grapevine samples of 11 autochthonous grapevine cultivars were collected in 20 commercial vineyards. Preliminary screening by serological (DAS-ELISA) and molecular (RT-PCR) analyses for ArMV (arabis mosaic virus) and GFLV detection were conducted. Results obtained showed the absence of ArMV in all the samples analyzed, while 48 out of 617 samples gave positive results to GFLV, for a total of 9 out of 11 cultivars analyzed. Phylogenetic analyses carried out on the GFLV-CP gene of 18 Sicilian GFLV sequences selected in this study showed a certain degree of variability among the Sicilian isolates, suggesting a different origin, probably as a consequence of the continuous interchange of GFLV-infected propagating material with other Italian regions or viticultural areas located in other countries.

Grapevine deformation virus: Completion of the sequence and evidence on its origin from recombination events between Grapevine fanleaf virus and Arabis mosaic virus

2012 ◽  
Vol 166 (1-2) ◽  
pp. 136-140 ◽  
Author(s):  
Toufic Elbeaino ◽  
Michele Digiaro ◽  
Saba Ghebremeskel ◽  
Giovanni P. Martelli
Keyword(s):  
Mosaic Virus ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus

scholarly journals Serological study of toxoplasmosis spread among unmarried female university students using LAT, ELISA and IgG avidity

2016 ◽  
Vol 13 (4) ◽  
pp. 714-720
Author(s):  
Baghdad Science Journal
Keyword(s):  
Toxoplasma Gondii ◽  
Elisa Test ◽  
Latex Agglutination ◽  
Female Students ◽  
Infectious Agents ◽  
Serum Samples ◽  
Serological Tests ◽  
Worldwide Distribution ◽  
Igm Elisa ◽  
Positive Results

Toxoplasma gondii has a worldwide distribution and it is one of the most prevalent infectious agents in Iraq. The study was conducted on 200 serum samples of unmarried female university of students age ranged between 18 to 26 years to detect Toxoplasma gondii antibodies. The aim of this study was to detect T. gondii antibodies among unmarried female students in Iraqi universities using different serological tests. Seventy six (38%) serum samples out of 200 subjects were positive for toxoplasma antibodies by Latex agglutination test (LAT). Among 76 LAT sera positive ,only 58 (29%) serum samples were positive with toxoplasma IgG ELISA test , however , the results of IgM ELISA assay were positive only for 3 (1.5%) unmarried female sample .None of negative LAT serum samples gave positive results with neither IgG nor IgM ELISA.

scholarly journals Sanitary status of slovenian indigenous grapevine cultivar Refosk

OENO One ◽  
2005 ◽  
Vol 39 (1) ◽  
pp. 19 ◽  
Author(s):  
Irma Tomazic ◽  
Zora Korosec-Koruza ◽  
Natasa Petrovic
Keyword(s):  
Vitis Vinifera ◽  
Mosaic Virus ◽  
Vitis Vinifera L ◽  
Grapevine Virus ◽  
Grapevine Fanleaf Virus ◽  
Arabis Mosaic Virus ◽  
Disease Symptoms ◽  
Grapevine Cultivar ◽  
Grapevine Virus A ◽  
Grapevine Virus B

<p style="text-align: justify;">Sanitary status of visually selected and nonselected indigenous grapevine cultivar 'Refosk' (Vitis vinifera L. cv. 'Refosk') was evaluated. Nine viruses were tested by ELISA: Nepoviruses Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV), Closteroviruses Grapevine leafroll associated viruses 1 (GLRaV-1), 2 (GLRaV-2), 3 (GLRaV-3), and 6 (GLRaV-6), Grapevine fleck virus (GFkV) and Vitiviruses Grapevine virus A (GVA) and Grapevine virus B (GVB). All viruses except ArMV and GVB were detected. The vines of cv. ‘Refosk’ passing the visual selection were 50 % free of tested viruses in comparison to only 24 % virus-free vines of non-visually selected material. Visually selected plants revealed the highest incidence of virus GLRaV- 1 (37 %) and in addition to that a 15 % incidence of rugose wood (RW) disease symptoms.</p>

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