scholarly journals Properties of the larval neuromuscular junction in Drosophila melanogaster.

1976 ◽  
Vol 262 (1) ◽  
pp. 189-214 ◽  
Author(s):  
L Y Jan ◽  
Y N Jan
Keyword(s):  
Drosophila Melanogaster ◽  
Neuromuscular Junction ◽  
Larval Neuromuscular Junction

scholarly journals Input-Specific Plasticity and Homeostasis at the Drosophila Larval Neuromuscular Junction

Neuron ◽  
2017 ◽  
Vol 93 (6) ◽  
pp. 1388-1404.e10 ◽  
Author(s):  
Zachary L. Newman ◽  
Adam Hoagland ◽  
Krishan Aghi ◽  
Kurtresha Worden ◽  
Sabrina L. Levy ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Larval Neuromuscular Junction

scholarly journals Perlecan regulates bidirectional Wnt signaling at the Drosophila neuromuscular junction

2013 ◽  
Vol 200 (2) ◽  
pp. 219-233 ◽  
Author(s):  
Keisuke Kamimura ◽  
Kohei Ueno ◽  
Jun Nakagawa ◽  
Rie Hamada ◽  
Minoru Saitoe ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Neuromuscular Junction ◽  
Heparan Sulfate ◽  
Wnt Signaling ◽  
Nuclear Import ◽  
Heparan Sulfate Proteoglycans ◽  
Down Regulation ◽  
Bidirectional Signaling ◽  
Synaptic Boutons ◽  
Signaling Activity

Heparan sulfate proteoglycans (HSPGs) play pivotal roles in the regulation of Wnt signaling activity in several tissues. At the Drosophila melanogaster neuromuscular junction (NMJ), Wnt/Wingless (Wg) regulates the formation of both pre- and postsynaptic structures; however, the mechanism balancing such bidirectional signaling remains elusive. In this paper, we demonstrate that mutations in the gene of a secreted HSPG, perlecan/trol, resulted in diverse postsynaptic defects and overproduction of synaptic boutons at NMJ. The postsynaptic defects, such as reduction in subsynaptic reticulum (SSR), were rescued by the postsynaptic activation of the Frizzled nuclear import Wg pathway. In contrast, overproduction of synaptic boutons was suppressed by the presynaptic down-regulation of the canonical Wg pathway. We also show that Trol was localized in the SSR and promoted postsynaptic accumulation of extracellular Wg proteins. These results suggest that Trol bidirectionally regulates both pre- and postsynaptic activities of Wg by precisely distributing Wg at the NMJ.

scholarly journals The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function

2015 ◽  
Vol 26 (18) ◽  
pp. 3275-3288 ◽  
Author(s):  
Kate Koles ◽  
Emily M. Messelaar ◽  
Zachary Feiger ◽  
Crystal J. Yu ◽  
C. Andrew Frank ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Postsynaptic Membrane ◽  
Lipid Binding ◽  
Synaptic Function ◽  
Muscle Membrane ◽  
Membrane Remodeling ◽  
Cellular Functions ◽  
Synaptic Homeostasis ◽  
Larval Neuromuscular Junction ◽  
Lipid Binding Proteins

Membranes form elaborate structures that are highly tailored to their specialized cellular functions, yet the mechanisms by which these structures are shaped remain poorly understood. Here, we show that the conserved membrane-remodeling C-terminal Eps15 Homology Domain (EHD) protein Past1 is required for the normal assembly of the subsynaptic muscle membrane reticulum (SSR) at the Drosophila melanogaster larval neuromuscular junction (NMJ). past1 mutants exhibit altered NMJ morphology, decreased synaptic transmission, reduced glutamate receptor levels, and a deficit in synaptic homeostasis. The membrane-remodeling proteins Amphiphysin and Syndapin colocalize with Past1 in distinct SSR subdomains and collapse into Amphiphysin-dependent membrane nodules in the SSR of past1 mutants. Our results suggest a mechanism by which the coordinated actions of multiple lipid-binding proteins lead to the elaboration of increasing layers of the SSR and uncover new roles for an EHD protein at synapses.

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