Determination of Cellular Mechanical Properties by Cell Poking, With an Application to Leukocytes

1990 ◽  
Vol 112 (3) ◽  
pp. 283-294 ◽  
Author(s):  
G. I. Zahalak ◽  
W. B. McConnaughey ◽  
E. L. Elson
Keyword(s):  
Mechanical Properties ◽  
Final Section ◽  
Mechanical Response ◽  
Living Cells ◽  
Micropipette Aspiration ◽  
Current Status ◽  
Technical Description ◽  
Analytical Results ◽  
Simple Models

In this paper we review the cell-poking technique as an approach for investigating the mechanical properties of living cells. We first summarize the rationale for the technique and the mainly qualitative results obtained so far. Then we provide a technical description of the instrument as it is configured at present. This is followed by a discussion of the current status of analytical results available for interpreting cell-poking measurements. In the final section we apply these results to an analysis of unmodulated and modulated lymphocytes and neutrophils, and conclude that the mechanical response of these leukocytes to indentation is not consistent with simple models developed by previous investigators on the basis of micropipette-aspiration experiments.

Determination of Natural Frequencies of Spherical Cells

2008 ◽  
Author(s):  
Marjan Molavi ◽  
Ali Bonakdar ◽  
Ion Stiharu
Keyword(s):  
Mechanical Properties ◽  
Finite Element ◽  
Modal Analysis ◽  
Natural Frequencies ◽  
Living Cells ◽  
Health Condition ◽  
Comprehensive Analysis ◽  
Yeast Cells ◽  
Spherical Cells

In this paper, a finite element (FE) and scaled-up experimental modal analysis are employed to estimate the natural frequencies of the baker’s yeast cells. It is apparent the mechanical properties of the living cells and particularly the natural frequencies are highly related to the health condition of cells, and therefore a comprehensive analysis is carried out to determine the natural frequencies of individual cells.

Validity Range of Micropipette Radius in Using Hemispherical Cap Model

2013 ◽  
Vol 419 ◽  
pp. 587-592
Author(s):  
G.Y. Jiao ◽  
Ming Dao ◽  
C.H. Sow ◽  
C.T. Lim
Keyword(s):  
Mechanical Properties ◽  
Mathematical Model ◽  
Mechanical Model ◽  
Living Cells ◽  
Micropipette Aspiration ◽  
Post Processing ◽  
Element Simulation ◽  
Validity Range ◽  
Cap Model ◽  
Experiment Technique

Micropipette aspiration is a widely used experiment technique for studying mechanical properties of living cells. Its data can be processed in many different ways. Among them, a mathematical model called hemispherical cap model has been developed to evaluate the elastic modulus of the cell membrane undergoing micropipette aspiration. It has been commonly used in cell studies due to its simplicity and convenience. However, this mechanical model has its own assumptions and limitations. In this article, the authors used finite element simulation to obtain the validity range of micropipette radius used in hemispherical cap model. The results provide reliable size instructions in the preparation and post-processing of micropipette experiments.

scholarly journals Determination of the viscoelastic properties of a single cell cultured on a rigid support by force microscopy

Nanoscale ◽  
2018 ◽  
Vol 10 (42) ◽  
pp. 19799-19809 ◽  
Author(s):  
Pablo D. Garcia ◽  
Ricardo Garcia
Keyword(s):  
Mechanical Properties ◽  
Single Cell ◽  
Viscoelastic Properties ◽  
Living Cells ◽  
Central Issue ◽  
Rigid Support ◽  
Force Microscopy ◽  
The Relationship

Understanding the relationship between the mechanical properties of living cells and physiology is a central issue in mechanobiology.

Basic set of experiments for determination of mechanical properties of sand

2014 ◽  
Vol 62 (1) ◽  
pp. 129-137
Author(s):  
A. Sawicki ◽  
J. Mierczyński
Keyword(s):  
Mechanical Properties ◽  
Soil Mechanics ◽  
Physical And Mechanical Properties ◽  
Local Strain ◽  
Initial State ◽  
Laboratory Equipment ◽  
Additional Information ◽  
Basic Set ◽  
Initial Anisotropy

Abstract A basic set of experiments for the determination of mechanical properties of sands is described. This includes the determination of basic physical and mechanical properties, as conventionally applied in soil mechanics, as well as some additional experiments, which provide further information on mechanical properties of granular soils. These additional experiments allow for determination of steady state and instability lines, stress-strain relations for isotropic loading and pure shearing, and simple cyclic shearing tests. Unconventional oedometric experiments are also presented. Necessary laboratory equipment is described, which includes a triaxial apparatus equipped with local strain gauges, an oedometer capable of measuring lateral stresses and a simple cyclic shearing apparatus. The above experiments provide additional information on soil’s properties, which is useful in studying the following phenomena: pre-failure deformations of sand including cyclic loading compaction, pore-pressure generation and liquefaction, both static and caused by cyclic loadings, the effect of sand initial anisotropy and various instabilities. An important feature of the experiments described is that they make it possible to determine the initial state of sand, defined as either contractive or dilative. Experimental results for the “Gdynia” model sand are shown.

Sign in / Sign up

Export Citation Format

Share Document