Characterization of Micro-Valves for Lab-on-Chip Device

2007 ◽  
Author(s):  
S. Dutta ◽  
D. Banerjee
Keyword(s):  
Water Quality ◽  
Molecular Recognition ◽  
Reaction Chamber ◽  
Nano Particles ◽  
Reaction Products ◽  
Lab On Chip ◽  
External Power Source ◽  
Gold Nano Particles ◽  
On Chip

The objective of this study is to develop a portable hand held diagnostics platform for monitoring pollutants and water quality testing. We are developing a lab-on-chip (LOC) device for in-situ synthesis of gold nano-particles and for using a colorimetric peptide assay for water quality monitoring. The gold nano-particles are synthesized in-situ in our experiments. The gold nano-particles exhibit various optical properties due to their Surface Plasmon Resonance (SPR). These stabilized mono-disperse gold nano-particles are coated with bio-molecular recognition motifs on their surfaces. The stabilization and functionalization with bio-molecular recognition motif provides flexibility for various applications. For example, the gold nano-particles synthesized by this process are tested for their ability to be recognized by a surface coated with anti-Flg antibodies. The LOC consists of micro-wells housing different reagents and samples that feed to a common reaction chamber. The reaction products are delivered to several waste chambers in a pre-defined sequence to enable subsequent reagents/ samples to flow into the reaction chamber. Passive flow actuation is obtained by capillary driven flow (wicking). Dissolvable micro-structures are used as passive micro-valves that actuate at predefined intervals and do not require any external power source for actuation. The microfluidic chip (LOC) and the dissolvable microstructures are fabricated using soft lithography techniques. The passive valves are incorporated into the microfluidics platform by novel micro-fabrication and bonding techniques.

Design and Test Issues for Lab-on-Chip Ion Separation for In-Situ Water Quality Monitoring

2012 ◽  
Author(s):  
Etienne Joly ◽  
Ian M. Bell ◽  
Ahmed Fallatah ◽  
Gillian M. Greenway ◽  
Stephen J. Haswell ◽  
...  
Keyword(s):  
Water Quality ◽  
Water Quality Monitoring ◽  
Quality Monitoring ◽  
Lab On Chip ◽  
Ion Separation ◽  
On Chip

scholarly journals The Rise of the OM-LoC: Opto-Microfluidic Enabled Lab-on-Chip

Micromachines ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1467
Author(s):  
Harry Dawson ◽  
Jinane Elias ◽  
Pascal Etienne ◽  
Sylvie Calas-Etienne
Keyword(s):  
Low Cost ◽  
Optical Components ◽  
New Era ◽  
Lab On Chip ◽  
Highly Sensitive ◽  
Multiple Challenges ◽  
On Chip ◽  
Optical Circuits ◽  
Made In

The integration of optical circuits with microfluidic lab-on-chip (LoC) devices has resulted in a new era of potential in terms of both sample manipulation and detection at the micro-scale. On-chip optical components increase both control and analytical capabilities while reducing reliance on expensive laboratory photonic equipment that has limited microfluidic development. Notably, in-situ LoC devices for bio-chemical applications such as diagnostics and environmental monitoring could provide great value as low-cost, portable and highly sensitive systems. Multiple challenges remain however due to the complexity involved with combining photonics with micro-fabricated systems. Here, we aim to highlight the progress that optical on-chip systems have made in recent years regarding the main LoC applications: (1) sample manipulation and (2) detection. At the same time, we aim to address the constraints that limit industrial scaling of this technology. Through evaluating various fabrication methods, material choices and novel approaches of optic and fluidic integration, we aim to illustrate how optic-enabled LoC approaches are providing new possibilities for both sample analysis and manipulation.

An autonomous lab-on-chip sensor for in situ measurements of seawater total alkalinity

2021 ◽  
Author(s):  
Allison Schaap ◽  
Stathys Papadimitriou ◽  
Socratis Loucaides ◽  
Matthew Mowlem
Keyword(s):  
In Situ Measurements ◽  
Total Alkalinity ◽  
Lab On Chip ◽  
On Chip

Hybrid Microgels Containing Gold Nanoparticles

e-Polymers ◽  
2006 ◽  
Vol 6 (1) ◽  
Author(s):  
Andrij Pich ◽  
Arpita Karak ◽  
Yan Lu ◽  
Anup K. Ghosh ◽  
Hans-Juergen P. Adler
Keyword(s):  
Gold Nanoparticles ◽  
Sodium Citrate ◽  
Colloidal Stability ◽  
Nano Particles ◽  
Composite Particles ◽  
Temperature Sensitive ◽  
Chloroauric Acid ◽  
Gold Nano Particles ◽  
Hybrid Microgels

AbstractIn present paper we report on a preparation of hybrid microgels filled with gold nano-particles (AuNPs). Temperature-sensitive VCL/AAEM microgels have been used as containers for deposition of Au by in situ formation of gold nano-particles. Synthesis of AuNPs has been performed by two methods: a) reduction of chloroauric acid with sodium borhydride and b) reduction of chloroauric acid by sodium citrate under ultrasonic agitation. In both cases AuNPs were deposited directly into microgels leading to formation of composite particles which exhibit temperature-sensitive properties, tunable gold contents and high colloidal stability. The influence of Au load on microgel size, morphology, swellingdeswelling behaviour and stability is discussed.

scholarly journals Industry Partnership: Lab on Chip Chemical Sensor Technology for Ocean Observing

2021 ◽  
Vol 8 ◽  
Author(s):  
Matt Mowlem ◽  
Alexander Beaton ◽  
Robin Pascal ◽  
Allison Schaap ◽  
Socratis Loucaides ◽  
...  
Keyword(s):  
Chemical Sensor ◽  
Autonomous Underwater Vehicles ◽  
Product Line ◽  
The Arctic ◽  
Sensor Technology ◽  
Lab On Chip ◽  
Chip Technology ◽  
Wide Range ◽  
On Chip

We introduce for the first time a new product line able to make high accuracy measurements of a number of water chemistry parameters in situ: i.e., submerged in the environment including in the deep sea (to 6,000 m). This product is based on the developments of in situ lab on chip technology at the National Oceanography Centre (NOC), and the University of Southampton and is produced under license by Clearwater Sensors Ltd., a start-up and industrial partner in bringing this technology to global availability and further developing its potential. The technology has already been deployed by the NOC, and with their partners worldwide over 200 times including to depths of ∼4,800 m, in turbid estuaries and rivers, and for up to a year in seasonally ice-covered regions of the arctic. The technology is capable of making accurate determinations of chemical and biological parameters that require reagents and which produce an electrical, absorbance, fluorescence, or luminescence signal. As such it is suitable for a wide range of environmental measurements. Whilst further parameters are in development across this partnership, Nitrate, Nitrite, Phosphate, Silicate, Iron, and pH sensors are currently available commercially. Theses sensors use microfluidics and optics combined in an optofluidic chip with electromechanical valves and pumps mounted upon it to mix water samples with reagents and measure the optical response. An overview of the sensors and the underlying components and technologies is given together with examples of deployments and integrations with observing platforms such as gliders, autonomous underwater vehicles and moorings.

scholarly journals Development of a lab-on-chip electrochemical biosensor for water quality analysis based on microalgal photosynthesis

2016 ◽  
Vol 79 ◽  
pp. 568-573 ◽  
Author(s):  
A. Tsopela ◽  
A. Laborde ◽  
L. Salvagnac ◽  
V. Ventalon ◽  
E. Bedel-Pereira ◽  
...  
Keyword(s):  
Water Quality ◽  
Electrochemical Biosensor ◽  
Quality Analysis ◽  
Water Quality Analysis ◽  
Lab On Chip ◽  
On Chip

scholarly journals Microoxygraph Device for Biosensoristic Applications

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
A. Aloisi ◽  
E. Tarentini ◽  
A. Ferramosca ◽  
V. Zara ◽  
R. Rinaldi
Keyword(s):  
Response Time ◽  
Low Cost ◽  
Reference Electrode ◽  
Reaction Chamber ◽  
Counter Electrodes ◽  
Permeable Membrane ◽  
Lab On Chip ◽  
Lift Off ◽  
On Chip

Oxygen consumption rate (OCR) is a significant parameter helpful to determinein vitrorespiratory efficiency of living cells. Oxygen is an excellent oxidant and its electrocatalytic reduction on a noble metal allows accurately detecting it. By means of microfabrication technologies, handy, low-cost, and disposable chip can be attained, minimizing working volumes and improving sensitivity and response time. In this respect, here is presented a microoxygraph device (MOD), based on Clark’s electrode principle, displaying many advantageous features in comparison to other systems. This lab-on-chip platform is composed of a three-microelectrode detector equipped with a microgrooved electrochemical cell, sealed with a polymeric reaction chamber. Au working/counter electrodes and Ag/AgCl reference electrode were fabricated on a glass slide. A microchannel was realized by photoresist lift-off technique and a polydimethylsiloxane (PDMS) nanoporous film was integrated as oxygen permeable membrane (OPM) between the probe and the microreaction chamber. Electrochemical measurements showed good reproducibility and average response time, assessed by periodic injection and suction of a reducing agent. OCR measurements on 3T3 cells, subjected, in real time, to chemical stress on the respiratory chain, were able to show that this chip allows performing consistent metabolic analysis.

scholarly journals Lab-on-chip analyser for the in situ determination of dissolved manganese in seawater

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Felix Geißler ◽  
Eric P. Achterberg ◽  
Alexander D. Beaton ◽  
Mark J. Hopwood ◽  
Mario Esposito ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Ionic Surfactant ◽  
Lab On Chip ◽  
Inductively Coupled ◽  
Long Term Stability ◽  
In Situ Data ◽  
Laboratory Characterization ◽  
Plasma Mass Spectrometry ◽  
On Chip

AbstractA spectrophotometric approach for quantification of dissolved manganese (DMn) with 1-(2-pyridylazo)-2-naphthol (PAN) has been adapted for in situ application in coastal and estuarine waters. The analyser uses a submersible microfluidic lab-on-chip device, with low power (~ 1.5 W) and reagent consumption (63 µL per sample). Laboratory characterization showed an absorption coefficient of 40,838 ± 1127 L⋅mol−1⋅cm−1 and a detection limit of 27 nM, determined for a 34.6 mm long optical detection cell. Laboratory tests showed that long-term stability of the PAN reagent was achieved by addition of 4% v/v of a non-ionic surfactant (Triton-X100). To suppress iron (Fe) interferences with the PAN reagent, the Fe(III) masking agents deferoxamine mesylate (DFO-B) or disodium 4,5-dihydroxy-1,3-benzenedisulfonate (Tiron) were added and their Fe masking efficiencies were investigated. The analyser was tested during a deployment over several weeks in Kiel Fjord (Germany), with successful acquisition of 215 in situ data points. The time series was in good agreement with DMn concentrations determined from discretely collected samples analysed via inductively coupled plasma mass spectrometry (ICP-MS), exhibiting a mean accuracy of 87% over the full deployment duration (with an accuracy of > 99% for certain periods) and clear correlations to key hydrographic parameters.

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