scholarly journals Evaluation of an Immunochromatography Test Using Enzyme Immunoassay for Rapid Detection of Influenza A and B Viruses

2004 ◽  
Vol 78 (7) ◽  
pp. 597-603 ◽  
Author(s):  
Keiko MITAMURA ◽  
Masahiko YAMAZAKI ◽  
Masataka ICHIKAWA ◽  
Kazuhiro KIMURA ◽  
Chiharu KAWAKAMI ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Rapid Detection ◽  
Influenza A

Rapid detection of influenza A neuraminidase subtypes by cDNA amplification coupled to a simple DNA enzyme immunoassay

1994 ◽  
Vol 139 (3-4) ◽  
pp. 439-444 ◽  
Author(s):  
B. Schweiger ◽  
I. Lange ◽  
R. Heckler ◽  
H. Willers ◽  
E. Schreier
Keyword(s):  
Enzyme Immunoassay ◽  
Rapid Detection ◽  
Influenza A ◽  
Dna Enzyme Immunoassay

Development of an antigen-ELISA and a colloidal gold–based immunochromatographic strip based on monoclonal antibodies for detection of avian influenza A(H5) viruses

2021 ◽  
pp. 104063872110275
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Linfang Cheng ◽  
Hangping Yao ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Disease Virus ◽  
Colloidal Gold ◽  
Rapid Detection ◽  
Influenza A ◽  
Field Investigation ◽  
Cross Reactivity ◽  
Detection Methods ◽  
Immunochromatographic Strip ◽  
Antigen Elisa

Avian influenza A(H5) viruses (avian IAVs) pose a major threat to the economy and public health. We developed an antigen-ELISA (ag-ELISA) and a colloidal gold–based immunochromatographic strip for the rapid detection of avian A(H5) viruses. Both detection methods displayed no cross-reactivity with other viruses (e.g., other avian IAVs, infectious bursal disease virus, Newcastle disease virus, infectious bronchitis virus, avian paramyxovirus). The ag-ELISA was sensitive down to 0.5 hemagglutinin (HA) units/100 µL of avian A(H5) viruses and 7.5 ng/mL of purified H5 HA proteins. The immunochromatographic strip was sensitive down to 1 HA unit/100 µL of avian A(H5) viruses. Both detection methods exhibited good reproducibility with CVs < 10%. For 200 random poultry samples, the sensitivity and specificity of the ag-ELISA were 92.6% and 98.8%, respectively, and for test strips were 88.9% and 98.3%, respectively. Both detection methods displayed high specificity, sensitivity, and stability, making them suitable for rapid detection and field investigation of avian A(H5) viruses.

PCR of DNA from dried blood spots on filter paper coupled to a simple DNA enzyme immunoassay for rapid detection of HIV-1

1996 ◽  
Vol 52 (4) ◽  
pp. 308-309
Author(s):  
B. Schweiger ◽  
C. Kücherer ◽  
C. Fleischer ◽  
H. v. Spreckelsen ◽  
P. Zablocki-Kaiser ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Rapid Detection ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Dna Enzyme Immunoassay ◽  
Hiv 1

scholarly journals Automated multiplex nucleic acid tests for rapid detection of SARS-CoV-2, influenza A and B infection with direct reverse-transcription quantitative PCR (dirRT-qPCR) assay in a centrifugal microfluidic platform

RSC Advances ◽  
2020 ◽  
Vol 10 (56) ◽  
pp. 34088-34098
Author(s):  
Minghui Ji ◽  
Yun Xia ◽  
Jacky Fong-Chuen Loo ◽  
Lang Li ◽  
Ho-Pui Ho ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Influenza A ◽  
Qpcr Assay ◽  
Multiplex Detection ◽  
Microfluidic Platform ◽  
Reverse Transcription Quantitative Pcr ◽  
Nucleic Acid Tests

Development of a microfluidic disc-direct reverse-transcription quantitative PCR platform to perform automated multiplex nucleic acid tests for rapid multiplex detection of disease infection.

scholarly journals Rapid Detection of Influenza A Infection in a Children's Hospital

1999 ◽  
Vol 45 (4, Part 2 of 2) ◽  
pp. 169A-169A
Author(s):  
Daniel E Noyola ◽  
Gail J Demmler
Keyword(s):  
Rapid Detection ◽  
Influenza A ◽  
Children's Hospital ◽  
Children’S Hospital

scholarly journals QuickVue Influenza Test for Rapid Detection of Influenza A and B Viruses in a Pediatric Population

2002 ◽  
Vol 9 (4) ◽  
pp. 925-926 ◽  
Author(s):  
Caroline Quach ◽  
Diane Newby ◽  
Ghislaine Daoust ◽  
Earl Rubin ◽  
Jane McDonald
Keyword(s):  
Cell Culture ◽  
Respiratory Tract ◽  
Rapid Detection ◽  
Influenza A ◽  
Respiratory Tract Infections ◽  
Pediatric Population ◽  
Lateral Flow Immunoassay ◽  
Viral Transport ◽  
Viral Transport Medium ◽  
Tract Infections

ABSTRACT The performance of a lateral-flow immunoassay, the QuickVue Influenza Test, for detection of influenza A and B viruses in comparison with that of cell culture was evaluated by using nasopharyngeal aspirates, in viral transport medium, from children with respiratory tract infections. The sensitivity and specificity were 79.2 and 82.6%, respectively.

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