Observation of live cells' attachment, spreading, and drug interactions using metasurface-enhanced infrared reflection spectroscopy

2019 ◽  
Author(s):  
Steven H. Huang ◽  
Shourya Dutta Gupta ◽  
Robert Delgado ◽  
Glen Kelp ◽  
Kelly Hume ◽  
...  
Keyword(s):  
Drug Interactions ◽  
Live Cells ◽  
Reflection Spectroscopy ◽  
Infrared Reflection ◽  
Infrared Reflection Spectroscopy

Monitoring the effects of chemical stimuli on live cells with metasurface-enhanced infrared reflection spectroscopy

2021 ◽  
Author(s):  
Steven H. Huang ◽  
Jiaruo Li ◽  
Zhiyuan Fan ◽  
Robert Delgado ◽  
Gennady Shvets
Keyword(s):  
Intracellular Signaling ◽  
Strong Field ◽  
Detection System ◽  
Field Enhancement ◽  
Chemical Imaging ◽  
Live Cells ◽  
Label Free ◽  
Reflection Spectroscopy ◽  
Infrared Reflection ◽  
Infrared Reflection Spectroscopy

Infrared spectroscopy has found wide applications in the analysis of biological materials. A more recent development is the use of engineered nanostructures, or plasmonic metasurfaces, as substrates for metasurface-enhanced infrared reflection spectroscopy (MEIRS). Here, we demonstrate that strong field enhancement from plasmonic metasurfaces enables the use of MEIRS as a highly informative analytic technique for real-time monitoring of cells. By exposing live cells cultured on a plasmonic metasurface to chemical compounds, we show that MEIRS can be used as a label-free phenotypic assay for detecting multiple cellular responses to external stimuli: changes in cell morphology, adhesion, lipid composition of the cellular membrane, as well as intracellular signaling. Using a focal plane array detection system, we show that MEIRS also enables spectro-chemical imaging at the single-cell level. The described metasurface-based all-optical sensor opens the way to a scalable, high-throughput spectroscopic assay for live cells.

scholarly journals Nanoplasmonic sensor for monitoring the effects of chemical stimuli on live cells using metasurface-enhanced infrared reflection spectroscopy

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Steven H. Huang ◽  
Jiaruo Li ◽  
Zhiyuan Fan ◽  
Robert Delgado ◽  
Gennady Shvets
Keyword(s):  
Infrared Spectroscopy ◽  
Biological Materials ◽  
Live Cells ◽  
Reflection Spectroscopy ◽  
Infrared Reflection ◽  
Chemical Stimuli ◽  
Infrared Reflection Spectroscopy

Infrared spectroscopy has found wide applications in the analysis of biological materials. A more recent development is the use of engineered nanostructures – plasmonic metasurfaces – as substrates for metasurface-enhanced...

Infrared spectroscopy of live cells from a flowing solution using electrically-biased plasmonic metasurfaces

Lab on a Chip ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 2136-2153 ◽  
Author(s):  
Glen Kelp ◽  
Joy Li ◽  
Junlan Lu ◽  
Nicholas DiNapoli ◽  
Robert Delgado ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Live Cells ◽  
Reflection Spectroscopy ◽  
Infrared Reflection ◽  
Infrared Reflection Spectroscopy ◽  
Flowing Solution

Microfluidics, dielectrophoresis (DEP) and metasurface-enhanced infrared reflection spectroscopy (MEIRS) are combined for capturing and rapid spectroscopy of live cells.

Noncontact infrared reflection spectroscopy using optical fibers

1994 ◽  
Vol 65 (8) ◽  
pp. 2731-2732 ◽  
Author(s):  
D. Bunimovich ◽  
E. Belotserkovsky ◽  
A. Katzir
Keyword(s):  
Optical Fibers ◽  
Reflection Spectroscopy ◽  
Infrared Reflection ◽  
Infrared Reflection Spectroscopy
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