Study of photosensitizers pharmacokinetics in mouse tumor model by transillumination fluorescence imaging in vivo

2011 ◽  
Author(s):  
Marina V. Shirmanova ◽  
Irina V. Balalaeva ◽  
Marina A. Sirotkina ◽  
Natalya Yu. Lekanova ◽  
Ilya V. Turchin ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Tumor Model ◽  
Mouse Tumor ◽  
Mouse Tumor Model

scholarly journals Nuclear FOXP3 inhibits tumor growth and induced apoptosis in hepatocellular carcinoma by targeting c-Myc

Oncogenesis ◽  
2020 ◽  
Vol 9 (10) ◽  
Author(s):  
Zhongqin Gong ◽  
Hao Jia ◽  
Jianqing Yu ◽  
Yi Liu ◽  
Jianwei Ren ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Model ◽  
Inhibitory Effect ◽  
Luciferase Assay ◽  
Migration And Invasion ◽  
Mouse Tumor ◽  
Clinicopathologic Characteristics ◽  
Mouse Tumor Model ◽  
Inhibitory Function

Abstract The status of FOXP3 and its isoforms in hepatocellular carcinoma (HCC) is unclear. We aimed to investigate the expression and function of FOXP3 and its isoforms in HCC. The study was performed on 84 HCC patients, HCC cell lines and a mouse tumor model. The levels of FOXP3 and its isoforms were determined by nested PCR, quantitative real-time PCR and immunohistochemistry (IHC) staining. The correlation between their levels and clinicopathologic characteristics was analyzed. The full length of FOXP3 (FOXP3) and exon 3-deleted FOXP3 (FOXP3Δ3) were found to be the major isoforms in HCC. The levels of FOXP3Δ3 mRNA and protein in HCC tumor samples were not significantly different from their adjacent normal tissues. The high expression of FOXP3 protein in HCC patients showed a good overall survival. The overexpression of FOXP3 significantly reduced tumor cell proliferation, migration and invasion. The immunofluorescence result indicated that FOXP3 needed to be translocated into the nucleus to exert its inhibitory function. The luciferase assay demonstrated that FOXP3 could be synergistic with Smad2/3/4 to inhibit the oncogene c-Myc. The co-immunoprecipitation results further revealed that FOXP3 could interact with Smad2/3/4. The chromatin immunoprecipitation (ChIP) assay showed that both FOXP3 and Smad2/3/4 bound the promoter of the c-Myc to inhibit it. The in vivo mouse tumor model study confirmed the inhibitory effect of FOXP3. Collectively, the expression of tumor FOXP3 can inhibit the growth of HCC via suppressing c-Myc directly or indirectly via interacting with Smad2/3/4. Therefore, FOXP3 is a tumor suppressor in HCC.

Generation of a Nude Mouse Tumor Model for In Vivo Replication of Human Cytomegalovirus

1998 ◽  
Vol 177 (3) ◽  
pp. 523-528 ◽  
Author(s):  
Gregory S. Pari ◽  
Dale Netski ◽  
Stephen St. Jeor ◽  
Donna McCarthy ◽  
Jean Smith ◽  
...  
Keyword(s):  
Nude Mouse ◽  
Human Cytomegalovirus ◽  
Tumor Model ◽  
Mouse Tumor ◽  
Mouse Tumor Model

scholarly journals Microcomputed tomography colonography for polyp detection in an in vivo mouse tumor model

2005 ◽  
Vol 102 (9) ◽  
pp. 3419-3422 ◽  
Author(s):  
P. J. Pickhardt ◽  
R. B. Halberg ◽  
A. J. Taylor ◽  
B. Y. Durkee ◽  
J. Fine ◽  
...  
Keyword(s):  
Tumor Model ◽  
Microcomputed Tomography ◽  
Mouse Tumor ◽  
Polyp Detection ◽  
Mouse Tumor Model

scholarly journals FOXP3 inhibits tumor growth and induced apoptosis in hepatocellular carcinoma by targeting c-Myc

2020 ◽  
Author(s):  
Zhongqin Gong ◽  
Hao Jia ◽  
Jianqing Yu ◽  
Yi Liu ◽  
Jianwei Ren ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Model ◽  
Inhibitory Effect ◽  
Luciferase Assay ◽  
Migration And Invasion ◽  
Mouse Tumor ◽  
Clinicopathologic Characteristics ◽  
Mouse Tumor Model ◽  
Inhibitory Function

Abstract Background The status of FOXP3 and its isoforms in hepatocellular carcinoma (HCC) is unclear. We aimed to investigate the expression and function of FOXP3 and its isoforms in HCC. Methods The study was performed on 84 HCC patients, HCC cell lines and a mouse tumor model. The levels of FOXP3 and its isoforms were determined by nested PCR, quantitative real-time PCR and immunohistochemistry (IHC) staining. The correlation between their levels and clinicopathologic characteristics was analyzed. Results The full length of FOXP3 (FOXP3) and exon 3-deleted FOXP3 (FOXP3Δ3) were found to be the major isoforms in HCC. The levels of FOXP3Δ3 mRNA and protein in HCC tumor samples were not significantly different from their adjacent normal tissues. The high expression of FOXP3 protein in HCC patients showed a good overall survival. The overexpression of FOXP3 significantly reduced tumor cell proliferation, migration and invasion. The immunofluorescence result indicated that FOXP3 needed to be translocated into the nucleus to exert its inhibitory function. The luciferase assay demonstrated that FOXP3 could be synergistic with Smad2/3/4 to inhibit the oncogene c-Myc. The co-immunoprecipitation results further revealed that FOXP3 could interact with Smad2/3/4. The chromatin immunoprecipitation (ChIP) assay showed that both FOXP3 and Smad2/3/4 bound the promoter of the c-Myc to inhibit it. The in vivo mouse tumor model study confirmed the inhibitory effect of FOXP3. Conclusion Collectively, the expression of tumor FOXP3 can inhibit the growth of HCC via suppressing c-Myc directly or indirectly via interacting with Smad2/3/4. Therefore, FOXP3 is a tumor suppressor in HCC.

scholarly journals In vivo photoacoustic monitoring using 700-nm region Raman source for targeting Prussian blue nanoparticles in mouse tumor model

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Nhat Quang Bui ◽  
Soon-Woo Cho ◽  
Madhappan Santha Moorthy ◽  
Sang Min Park ◽  
Zhonglie Piao ◽  
...  
Keyword(s):  
Prussian Blue ◽  
Tumor Model ◽  
Mouse Tumor ◽  
Mouse Tumor Model ◽  
Prussian Blue Nanoparticles

In Vivo Biodistribution of Dendrimers and Dendrimer Nanocomposites — Implications for Cancer Imaging and Therapy

2005 ◽  
Vol 4 (6) ◽  
pp. 603-613 ◽  
Author(s):  
Mohamed K. Khan ◽  
Shraddha S. Nigavekar ◽  
Leah D. Minc ◽  
Muhammed S. T. Kariapper ◽  
Bindu M. Nair ◽  
...  
Keyword(s):  
Cancer Imaging ◽  
Tumor Model ◽  
Mouse Tumor ◽  
Mouse Tumor Model ◽  
In Vivo Biodistribution ◽  
Structure Of Nanoparticles ◽  
Toxicity Analysis ◽  
Tumor Model System ◽  
Near Future

Our results indicate that the surface chemistry, composition, and 3-D structure of nanoparticles are critical in determining their in vivo biodistribution, and therefore the efficacy of nanodevice imaging and therapies. We demonstrate that gold/dendrimer nanocomposites in vivo, present biodistribution characteristics different from PAMAM dendrimers in a B16 mouse tumor model system. We review important chemical and biologic uses of these nanodevices and discuss the potential of nanocomposite devices to greatly improve cancer imaging and therapy, in particular radiation therapy. We also discuss major issues confronting the use of nanoparticles in the near future, with consideration of toxicity analysis and whether biodegradable devices are needed or even desirable.

Identification of circulating microrna pattern alteration during the tumor development using C38/C57BL/6 mouse tumor model

2012 ◽  
Vol 50 (05) ◽  
Author(s):  
Z Nagy ◽  
B Barták ◽  
S Spisák ◽  
B Wichmann ◽  
A Kalmár ◽  
...  
Keyword(s):  
Tumor Development ◽  
Tumor Model ◽  
Circulating Microrna ◽  
Mouse Tumor ◽  
Mouse Tumor Model

scholarly journals Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials

EBioMedicine ◽  
2021 ◽  
Vol 67 ◽  
pp. 103345
Author(s):  
Ryuhei Okada ◽  
Aki Furusawa ◽  
Daniel W. Vermeer ◽  
Fuyuki Inagaki ◽  
Hiroaki Wakiyama ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Near Infrared ◽  
Tumor Model ◽  
Mouse Tumor ◽  
Mouse Tumor Model

Functional Genomics Guided with MR Imaging: Mouse Tumor Model Study

Radiology ◽  
2003 ◽  
Vol 228 (2) ◽  
pp. 560-568 ◽  
Author(s):  
Samira Guccione ◽  
Yi-Shan Yang ◽  
Gongyi Shi ◽  
Daniel Y. Lee ◽  
King C. P. Li ◽  
...  
Keyword(s):  
Mr Imaging ◽  
Functional Genomics ◽  
Tumor Model ◽  
Mouse Tumor ◽  
Mouse Tumor Model ◽  
Model Study
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