scholarly journals Genetic characterization of a Clostridium difficile erythromycin-clindamycin resistance determinant that is transferable to Staphylococcus aureus.

1987 ◽  
Vol 31 (7) ◽  
pp. 1039-1045 ◽  
Author(s):  
H Hachler ◽  
B Berger-Bachi ◽  
F H Kayser
Keyword(s):  
Staphylococcus Aureus ◽  
Clostridium Difficile ◽  
Genetic Characterization ◽  
Resistance Determinant

Genetic characterization of two methicillin-resistant Staphylococcus aureus spa type t127 strains isolated from workers in the dairy production chain in Greece

2021 ◽  
Author(s):  
Theodoros Karampatakis ◽  
Panagiotis Papadopoulos ◽  
Katerina Tsergouli ◽  
Apostolos S. Angelidis ◽  
Daniel Sergelidis ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Characterization ◽  
Food Handler ◽  
Dairy Production ◽  
Whole Genome ◽  
Genome Sequences ◽  
Production Chain ◽  
Methicillin Resistant ◽  
Mrsa Strains

AbstractMethicillin-resistant Staphylococcus aureus (MRSA) constitutes a constant threat for the public health. Aim of the present study was to analyse the whole genome sequences of two MRSA strains belonging to Staphylococcus protein A (spa) type t127 isolated from humans working in two distantly located dairy production farms in Greece.MRSA strains were isolated from the nasal cavity of a food handler in a milk industry in Epirus, northwestern Greece (E-MRSA), and a person working in a cattle farm in Thrace, northeastern Greece (T-MRSA). Whole genome sequences taken using next generation sequencing were analysed for resistance and virulence genes applying various bioinformatic tools.Both isolates were assigned to ST1-IVa-t127 type, and they were transferring genes conferring resistance to tetracycline, β-lactams, and aminoglycosides; T-MRSA was carrying additional genes leading to macrolide, lincosamide and streptogramin B (MLSB) resistance. Both isolates were carrying three plasmid replicon types, rep5, rep7 and rep16, while T-MRSA harboured also rep10 and rep15. E-MRSA carried scn and sak genes which were absent from T-MRSA.In conclusion, the genetic characterization of two unrelated ST1-IVa-t127 MRSA strains isolated from humans in close contact with livestock in Greece can be used as basis for further epidemiological and evolutionary studies.

Genetic characterization of livestock-associated methicillin-resistant Staphylococcus aureus isolated in Greece

2021 ◽  
Author(s):  
Theodoros Karampatakis ◽  
Panagiotis Papadopoulos ◽  
Katerina Tsergouli ◽  
Apostolos S. Angelidis ◽  
Angeliki Melidou ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Genetic Characterization ◽  
Methicillin Resistant

Genetic characterization of coagulase-positive staphylococci isolated from healthy pigeons

2015 ◽  
Vol 18 (3) ◽  
pp. 627-634 ◽  
Author(s):  
M. Kizerwetter-Świda ◽  
D. Chrobak-Chmiel ◽  
M. Rzewuska ◽  
A. Antosiewicz ◽  
B. Dolka ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Relatedness ◽  
Genetic Characterization ◽  
Restriction Patterns ◽  
Feral Pigeons ◽  
Group A ◽  
Biochemical Identification ◽  
Group B ◽  
Coagulase Positive Staphylococci

AbstractCoagulase-positive staphylococci (CoPS) are opportunistic veterinary pathogens, of whichStaphylococcus aureus,S. delphiniandS. intermediuscan be isolated from pigeons. The biochemical identification ofS. delphiniandS. intermediusisolates may be incorrect, because of their phenotypic similarity. The purpose of the present study was to isolate and identify CoPS from domestic and feral pigeons and to determine their genetic relatedness by PFGE. A total number of 31 isolates of CoPS were obtained, 15 were identified asS. delphinigroup B, six asS. aureus,four asS. delphinigroup A, three asS. intermediusand three asS. schleiferisubsp.coagulans. The results indicate that S.delphinigroup B is the predominant CoPS species among pigeons studied. PFGE restriction patterns ofS. delphinigroup A andS. delphinigroup B form separate clusters, demonstrating their genetic heterogeneity. Indistinguishable or very similar PFGE patterns observed amongS. delphinigroup B isolates from domestic and feral pigeons confirm the possibility of CoPS transmission between these birds.

scholarly journals Occurrence and genetic characterization of Staphylococcus aureus in milk samples of cattle with mastitis, and in the Veterinary Hospital personnel and dairy workers

2017 ◽  
Vol 54 (2) ◽  
pp. 117
Author(s):  
Lucianne Leigue ◽  
Ayrton Rodrigo Hilgert ◽  
Adriana Fiorini ◽  
Marise Fonseca dos Santos ◽  
Eliane Cristina Gruzka Vendruscolo
Keyword(s):  
Staphylococcus Aureus ◽  
Genetic Characterization ◽  
Hospital Personnel ◽  
Milk Samples ◽  
Dairy Workers ◽  
Veterinary Hospital

Staphylococcus aureus está entre os microrganismos que apresentam as maiores taxas de morbidade e mortalidade em seres humanos e animais. Linhagens de S. aureus resistentes a meticilina podem causar surtos de infecção em todo o mundo, o que contribui para a escassez de arsenal terapêutico. Este trabalho analisou o perfil epidemiológico de estirpes de S. aureus isoladas de pessoas que trabalham em contato com animais em um hospital veterinário com gado leiteiro e também em amostras de leite de vacas acometidas por mastite. As estirpes de S. aureus isoladas foram caracterizadas fenotipicamente por meio de antibiograma, testes de catalase e coagulase, e pelo teste de Voges-Proskauer. As amostras também foram caracterizadas genotipicamente pela técnica de Analise de Restricao de DNA Ribossomico Amplificado (ARDRA-PCR). Das 218 estirpes isoladas, 27 foram identificados como S. aureus (12%). Entre essas, quatro estirpes foram resistentes a oxacilina e duas classificadas como SARM (S. aureus resistente a meticilina). A ocorrência de estirpes de S.aureus isoladas entre o pessoal que trabalha em contato com os animais foi baixa (2%), mas estirpes identificadas como SARM foram encontradas na equipe clínica. As análises de ARDRA realizadas com as enzimas de restrição HindIII e HinfII demonstraram que S. aureus isolados de diferentes indivíduos que trabalhavam com animais foram agrupados no mesmo cluster. Quando a ARDRA foi realizada com HaeIII foi observada formação de dois grupos, mas os isolados não se correlacionaram. Conclusão: a ocorrência de estirpes de S. aureus isoladas foi maior na equipe clínica, apresentando também correlação de 100% nos ensaios bioquímicos e moleculares.

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