scholarly journals Enhanced Triacylglycerol Metabolism Contributes to Efficient Oil Utilization and High-Level Production of Salinomycin in Streptomyces albus ZD11

2020 ◽  
Vol 86 (16) ◽  
Author(s):  
Han Li ◽  
Jiaxiu Wei ◽  
Jianxin Dong ◽  
Yudong Li ◽  
Yongquan Li ◽  
...  
Keyword(s):  
Carbon Source ◽  
High Efficiency ◽  
Antibiotic Biosynthesis ◽  
Comparative Genomic ◽  
Streptomyces Albus ◽  
Main Carbon Source ◽  
Oil Utilization ◽  
High Level ◽  
Level Production ◽  
Main Carbon

ABSTRACT Streptomyces is well known for biosynthesis of secondary metabolites with diverse bioactivities. Although oils have been employed as carbon sources to produce polyketide antibiotics for several industrial Streptomyces strains, the intrinsic correlation between oil utilization and high production of antibiotics still remains unclear. In this study, we investigated the correlation between oil metabolism and salinomycin biosynthesis in Streptomyces albus ZD11, which employs soybean oil as the main carbon source. Comparative genomic analysis revealed the enrichment of genes related to triacylglycerol (TAG) metabolism in S. albus ZD11. Transcriptomic profiling further confirmed the enhancement of TAG metabolism and acyl coenzyme A biosynthesis in S. albus ZD11. Multiple secreted lipases, which catalyze TAG hydrolysis, were seen to be working in a synergistic and complementary manner in aiding the efficient and stable hydrolyzation of TAGs. Together, our results suggest that enhanced TAG hydrolysis and fatty acid degradation contribute to the high efficiency of oil utilization in S. albus ZD11 in order to provide abundant carbon precursors for cell growth and salinomycin biosynthesis. IMPORTANCE In order to obtain high-level production of antibiotics, oils have been used as the main carbon source for some Streptomyces strains. Based on multiomics analysis, this study provides insight into the relationship between triacylglycerol (TAG) metabolism and antibiotic biosynthesis in S. albus ZD11, an oil-preferring industrial Streptomyces strain. Our investigation into TAG hydrolysis yielded further evidence that this strain utilizes complicated strategies enabling an efficient TAG metabolism. In addition, a novel secreted lipase was identified that exhibited highly hydrolytic activity for medium- and long-chain TAGs. Our findings represent a good start toward clarifying the complicated relationship between TAG catabolism and high-level antibiotic production in the industrial strains.

scholarly journals A β-d-fructofuranosidase from Claviceps purpurea

1972 ◽  
Vol 129 (2) ◽  
pp. 263-272 ◽  
Author(s):  
A. G. Dickerson
Keyword(s):  
Carbon Source ◽  
Dual Role ◽  
Claviceps Purpurea ◽  
Time Transfer ◽  
Main Carbon Source ◽  
Main Carbon

Evidence suggests that sucrose is the main carbon source for growth of Claviceps spp. in the parasitic condition. The sucrose acts as substrate for an active β-fructofuranosidase, produced by the fungus, which in the first instance converts the disaccharide into glucose and an oligofructoside. In this way, 50% of the glucose, supplied as sucrose, is made available to the parasite for assimilation. Subsequent action of the enzyme on both sucrose and the oligofructoside leads to the release of more glucose and the formation of additional oligosaccharides. The structures of the main oligosaccharides formed have been elucidated and the interactions of each compound studied. In experiments with purified enzyme in vitro the interaction of the oligosaccharides is rapid but in culture they are assimilated only slowly; in each case some free fructose is liberated. Free fructose is not assimilated in the presence of glucose and, further, inhibits growth at concentrations which might be expected to occur in the parasitic condition. A dual role has been suggested for the enzyme, with sucrose as substrate, in which glucose is made available to the growing parasite, while at the same time transfer of the fructose to form oligosaccharides prevents it from accumulating at inhibitory concentrations. Ultimately, when glucose becomes limiting, the fungus will adapt to fructose assimilation.

scholarly journals High level production of flavonoid rhamnosides by metagenome-derived Glycosyltransferase C in Escherichia coli utilizing dextrins of starch as a single carbon source

2019 ◽  
Vol 55 ◽  
pp. 212-219 ◽  
Author(s):  
Constantin Ruprecht ◽  
Friedericke Bönisch ◽  
Nele Ilmberger ◽  
Tanja V. Heyer ◽  
Erhard T.K. Haupt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbon Source ◽  
Single Carbon Source ◽  
High Level ◽  
Level Production

Screening of biosurfactant-producing Bacillus strains using glycerol from the biodiesel synthesis as main carbon source

2012 ◽  
Vol 35 (6) ◽  
pp. 897-906 ◽  
Author(s):  
M. Sousa ◽  
V. M. M. Melo ◽  
S. Rodrigues ◽  
H. B. Sant’ana ◽  
L. R. B. Gonçalves
Keyword(s):  
Carbon Source ◽  
Biodiesel Synthesis ◽  
Main Carbon Source ◽  
Bacillus Strains ◽  
Main Carbon

scholarly journals Influence of Carbon, Nitrogen and Phosphorous Sources on Glucoamylase Production by Aspergillus awamori in Solid State Fermentation

2003 ◽  
Vol 58 (9-10) ◽  
pp. 708-712 ◽  
Author(s):  
Telma Elita Bertolin ◽  
Willibaldo Schmidell ◽  
Alfredo E. Maiorano ◽  
Janice Casara ◽  
Jorge A. V. Costa
Keyword(s):  
Solid State ◽  
Carbon Source ◽  
Solid State Fermentation ◽  
Carbon Sources ◽  
Aspergillus Awamori ◽  
Main Carbon Source ◽  
Nitrogen Phosphorus ◽  
Additional Carbon Source ◽  
Main Carbon ◽  
Glucoamylase Production

AbstractIt was the objective of the present study to increase the production of glucoamylase by Aspergillus awamori through solid state fermentation, using wheat bran as the main carbon source and (NH4)2SO4, urea, KH2PO4, glucose, maltose and starch as additional nitrogen, phosphorus, and carbon sources. The production of glucoamylase is strongly influenced by N and C sources. A 100% increase was observed when the (NH4)2SO4 was replaced by urea, with C/N = 4.8, using maltose as the additional carbon source. C/P ratios in a range of 5.1 to 28.7 did not induce glucoamylase production under the studied conditions.

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