scholarly journals Correlation between Detection of a Plasmid and High-Level Virulence of Vibrio nigripulchritudo, a Pathogen of the Shrimp Litopenaeus stylirostris

2008 ◽  
Vol 74 (10) ◽  
pp. 3038-3047 ◽  
Author(s):  
Yann Reynaud ◽  
Denis Saulnier ◽  
Didier Mazel ◽  
Cyrille Goarant ◽  
Frédérique Le Roux
Keyword(s):  
Genetic Markers ◽  
New Caledonia ◽  
Subtractive Hybridization ◽  
Epidemiological Studies ◽  
Etiological Agent ◽  
Subtraction Library ◽  
High Virulence ◽  
High Level ◽  
Mass Mortalities ◽  
Coral Pathogen

ABSTRACT Vibrio nigripulchritudo, the etiological agent of Litopenaeus stylirostris summer syndrome, is responsible for mass mortalities of shrimp in New Caledonia. Epidemiological studies led to the suggestion that this disease is caused by an emergent group of pathogenic strains. Genomic subtractive hybridization was carried out between two isolates exhibiting low and high virulence. Our subtraction library was constituted of 521 specific fragments; 55 of these were detected in all virulent isolates from our collection (n = 32), and 13 were detected only in the isolates demonstrating the highest pathogenicity (n = 19), suggesting that they could be used as genetic markers for high virulence capacity. Interestingly, 10 of these markers are carried by a replicon of 11.2 kbp that contains sequences highly similar to those of a plasmid detected in Vibrio shilonii, a coral pathogen. The detection of this plasmid was correlated with the highest pathogenicity status of the isolates from our collection. The origin and consequence of this plasmid acquisition are discussed.

scholarly journals Whole-Genome Phylogenomic Heterogeneity of Neisseria gonorrhoeae Isolates with Decreased Cephalosporin Susceptibility Collected in Canada between 1989 and 2013

2014 ◽  
Vol 53 (1) ◽  
pp. 191-200 ◽  
Author(s):  
Walter Demczuk ◽  
Tarah Lynch ◽  
Irene Martin ◽  
Gary Van Domselaar ◽  
Morag Graham ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Large Scale ◽  
Epidemiological Studies ◽  
23S Rrna ◽  
Genome Comparison ◽  
Whole Genome ◽  
Content Type ◽  
Genomic Epidemiology ◽  
High Level ◽  
Sequence Types

A large-scale, whole-genome comparison of CanadianNeisseria gonorrhoeaeisolates with high-level cephalosporin MICs was used to demonstrate a genomic epidemiology approach to investigate strain relatedness and dynamics. Although current typing methods have been very successful in tracing short-chain transmission of gonorrheal disease, investigating the temporal evolutionary relationships and geographical dissemination of highly clonal lineages requires enhanced resolution only available through whole-genome sequencing (WGS). Phylogenomic cluster analysis grouped 169 Canadian strains into 12 distinct clades. While someN. gonorrhoeaemultiantigen sequence types (NG-MAST) agreed with specific phylogenomic clades or subclades, other sequence types (ST) and closely related groups of ST were widely distributed among clades. Decreased susceptibility to extended-spectrum cephalosporins (ESC-DS) emerged among a group of diverse strains in Canada during the 1990s with a variety of nonmosaicpenAalleles, followed in 2000/2001 with thepenAmosaic X allele and then in 2007 with ST1407 strains with thepenAmosaic XXXIV allele. Five genetically distinct ESC-DS lineages were associated withpenAmosaic X, XXXV, and XXXIV alleles and nonmosaic XII and XIII alleles. ESC-DS with coresistance to azithromycin was observed in 5 strains with 23S rRNA C2599T or A2143G mutations. As the costs associated with WGS decline and analysis tools are streamlined, WGS can provide a more thorough understanding of strain dynamics, facilitate epidemiological studies to better resolve social networks, and improve surveillance to optimize treatment for gonorrheal infections.

ANALYSIS OF THE REASONS FOR THE REFUSAL OF THE POPULATION FROM VACCINES

Vestnik ◽  
2021 ◽  
pp. 280-284
Author(s):  
Х. Б. Сарсенбай ◽  
А. Н. Турсынханова ◽  
Б. А. Конысов
Keyword(s):  
Preschool Children ◽  
Young Children ◽  
Disease Prevention ◽  
Infectious Diseases ◽  
Respiratory Infections ◽  
Epidemiological Studies ◽  
Recurrent Respiratory Infections ◽  
High Level ◽  
Mandatory Vaccination

Согласно данным ВОЗ, современные эпидемиологические исследования показали, что рецидивирующие респираторные инфекции у детей раннего и дошкольного возраста сохраняются на высоком уровне 10-50% в течение более 40 лет. Конец вакцинации может спровоцировать массовые инфекционные заболевания. Врачи бьют тревогу, что число отказников от обязательной вакцинации растет. Только в каждой поликлинике Алматы 30-40 родителей в год говорят о готовности к профилактике заболеваний. Среди тех, кто категорически отказывается трогать своих детей иголками, - те, кто идет по пути религии. Они считают, что прививка противоречит убеждениям. В последнее время список недовольных групп пополняют и простые жители, которые скептически относятся к квалификации белых. According to WHO, current epidemiological studies have shown that recurrent respiratory infections in young children and preschool children persist at a high level of 10-50% for more than 40 years. The end of vaccination can provoke mass infectious diseases. Doctors are sounding the alarm that the number of refusers from mandatory vaccination is growing. Only in every polyclinic in Almaty, 30-40 parents a year talk about their readiness for disease prevention. Among those who categorically refuse to touch their children with needles are those who follow the path of religion. They believe that vaccination is contrary to their beliefs. Recently, the list of discontented groups has been supplemented by ordinary residents who are skeptical about the qualifications of whites.

scholarly journals THE EPIDEMIOLOGY OF FOWL CHOLERA

1930 ◽  
Vol 51 (2) ◽  
pp. 249-258 ◽  
Author(s):  
Ida W. Pritchett ◽  
F. R. Beaudette ◽  
T. P. Hughes
Keyword(s):  
Field Studies ◽  
Colony Type ◽  
Fowl Cholera ◽  
Poultry Farms ◽  
High Virulence ◽  
Commercial Poultry ◽  
The Individual ◽  
High Level ◽  
Serum Agglutination ◽  
Healthy Carriers

Field studies of fowl cholera on two commercial poultry farms are described. One farm, previously free of cholera, was studied during an active epidemic, which occurred during the winter months. The strains of P. avicida recovered, both from "autopsy" and from "healthy carriers" proved generally similar, and to be of the "fluorescent" or "intermediate" colony type, which is of relatively high virulence. After the subsidence of the epidemic, these strains tended to disappear. The second flock consisted of a small group of birds which had survived an epidemic of cholera the previous year, and in which the infection was prevailing in endemic form. No deaths occurred during the period of observation, but the number of birds with localized lesions and the number of carriers increased to a high level during the winter months. The strains of P. avicida were apparently of the "blue" colony form, although some, as shown by their acid and serum agglutination reactions, resembled the "intermediates." These strains appeared to be spreading rather than dying out. The individual fowls differed in their response to the presence of infection; some showed localized lesions, others were carriers, while still others seemed entirely refractory.

scholarly journals Persistent Changes in Gene Expression Induced by Estrogen and Progesterone in the Rat Mammary Gland

2001 ◽  
Vol 15 (11) ◽  
pp. 1993-2009 ◽  
Author(s):  
Melanie R. Ginger ◽  
Maria F. Gonzalez-Rimbau ◽  
Jason P. Gay ◽  
Jeffrey M. Rosen
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Cell Fate ◽  
Noncoding Rna ◽  
Previous Treatment ◽  
Hormone Treatment ◽  
Subtractive Hybridization ◽  
Epidemiological Studies ◽  
Altered Expression ◽  
Hormonal Milieu

Abstract Epidemiological studies have consistently shown that an early full-term pregnancy is protective against breast cancer. We hypothesize that the hormonal milieu that is present during pregnancy results in persistent changes in the pattern of gene expression in the mammary gland, leading to permanent changes in cell fate that determine the subsequent proliferative response of the gland. To investigate this hypothesis, we have used suppression subtractive hybridization to identify genes that are persistently up-regulated in the glands of E- and progesterone (P)-treated Wistar-Furth rats 28 d after steroid hormone treatment compared with age-matched virgins. Using this approach, a number of genes displaying persistent altered expression in response to previous treatment with E and P were identified. Two markers have been characterized in greater detail: RbAp46 and a novel gene that specifies a noncoding RNA (designated G.B7). Both were persistently up-regulated in the lobules of the regressed gland and required previous treatment with both E and P for maximal persistent expression. RbAp46 has been implicated in a number of complexes involving chromatin remodeling, suggesting a mechanism whereby epigenetic factors responsible for persistent changes in gene expression may be related to the determination of cell fate. These results provide the first support at the molecular level for the hypothesis that hormone-induced persistent changes in gene expression are present in the involuted mammary gland.

scholarly journals Taro vein chlorosis virus: characterization and variability of a new nucleorhabdovirus

2005 ◽  
Vol 86 (2) ◽  
pp. 491-499 ◽  
Author(s):  
Peter Revill ◽  
Xuan Trinh ◽  
James Dale ◽  
Rob Harding
Keyword(s):  
Pacific Islands ◽  
New Caledonia ◽  
Solomon Islands ◽  
N Gene ◽  
Nucleotide Level ◽  
Putative Gene ◽  
Intergenic Regions ◽  
Rna Genome ◽  
Characteristic 3 ◽  
High Level

Sequencing of the monopartite RNA genome of a Fijian isolate of Taro vein chlorosis virus (TaVCV) confirmed that it is a definitive rhabdovirus with most similarity to members of the genus Nucleorhabdovirus. The TaVCV 12 020 nt negative-sense RNA genome contained six ORFs in the antigenomic sequence, equivalent to the N, P, 3, M, G and L genes that have been identified in other rhabdoviruses. The putative gene products had highest similarity to those of the nucleorhabdovirus Maize mosaic virus. A characteristic 3′-AAUUCUUUUUGGGUUGU/A-5′ sequence was identified in each of the intergenic regions and the TaVCV leader and trailer sequences comprised 140 and 61 nt, respectively. Assignment of TaVCV to the genus Nucleorhabdovirus was supported by thin-section electron microscopy of TaVCV-infected taro leaves, which identified virions budding from nuclear membranes into the perinuclear space. Variability studies identified high levels of TaVCV sequence diversity. Within the L gene of 20 TaVCV isolates from Fiji, the Federated States of Micronesia, New Caledonia, Papua New Guinea, Solomon Islands and Vanuatu, maximum variability at the nucleotide level was 27·4 %. Within the N gene, maximum variability among 15 isolates at the nucleotide level was 19·3 %. The high level of TaVCV variability observed suggested that the introduction of TaVCV to the Pacific Islands was not a recent occurrence.

Objectives and Review of the International Milk Fluoridation Program

1995 ◽  
Vol 9 (2) ◽  
pp. 110-111 ◽  
Author(s):  
G.N. Pakhomo
Keyword(s):  
Dental Caries ◽  
National Level ◽  
Data Bank ◽  
Epidemiological Studies ◽  
Weighted Mean ◽  
Number Of Children ◽  
The World ◽  
Low Levels ◽  
High Level ◽  
Very High

The WHO Global Oral Data Bank (GODB) demonstrates wide varieties of dental caries levels. During the last 10 years, dental caries prevalence in many countries has decreased from very high and high to moderate and low levels. However, there also are countries where dental caries has increased from very low and low to a moderate level. In total in 1993, of the 158 countries for which the WHO GODB has data available, 16 countries indicate a very low level (69 - low, 53 - moderate, 17 - high), and only three a very high level of dental caries. Very high levels of dental caries have been recorded in Costa Rica, Jamaica, and Uruguay. All these data, based on the weighted mean of DMF in 12-year-old children, have been obtained from national surveys or collected from published papers on oral health surveys conducted in selected areas of the countries. Very often, these papers indicated an increase (or decrease) in dental caries in people living in different areas of a particular country; however, the DMF weighted mean at the national level is still without change. One of the most populated countries in the world, China, shows clear evidence from several recent epidemiological studies that the level of dental caries in the urban population is persistently increasing. Dental caries still remains one of the most common diseases affecting a substantial number of children and adults around the world. There is evidence that water or

scholarly journals Chemical Investigations of Essential Oils from Endemic Cupressaceae Trees from New Caledonia

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500
Author(s):  
Nicolas Lebouvier ◽  
Chantal Menut ◽  
Edouard Hnawia ◽  
Audrey Illinger ◽  
Pierre Cabalion ◽  
...  
Keyword(s):  
Essential Oils ◽  
New Caledonia ◽  
Volatile Components ◽  
Ms Analysis ◽  
Leaf Oil ◽  
High Level ◽  
First Time

The volatile components obtained by hydrodistillation of leaves of C. neocaledonica Dummer, C. sulcata (Parlatore) Schlechter and N. pancheri (Carrière) de Laubenfels from New Caledonia were investigated for the first time by a combination of GC and GC–MS analysis, and compared with the heartwood oil compositions of the three species. The essential oils from C. sulcata and C. neocaledonica leaves contain a majority of monoterpenes while the leaf oil of N. pancheri is characterized by a high level of sesquiterpenoids. On the basis of the sesquiterpenoid composition of the wood- and leaf oils, N. pancheri is closely related to both New Caledonian Callitris spp. However, C. sulcata and C. neocaledonica oils remain distinct from N. pancheri and the Australian Callitris oils by the presence of compounds biosynthetically related to the bisabolyl cation, mainly barbatenes and thujopsene.

Relationship of Socio Economic Status, Income, and Education with the Survival Rate of Breast Cancer: A Meta-Analysis

2020 ◽  
Author(s):  
Majid TAHERI ◽  
Mohammad TAVAKOL ◽  
Mohammad Esmaeil AKBARI ◽  
Amir ALMASI-HASHIANI ◽  
Mahmoud ABBASI
Keyword(s):  
Breast Cancer ◽  
Cancer Survival ◽  
Economic Status ◽  
Meta Analysis ◽  
Epidemiological Studies ◽  
Social Determinant ◽  
Breast Cancer Survival ◽  
Socio Economic Status ◽  
Academic Level ◽  
High Level

Background: Despite our awareness of the significant effect of Social Determinant of Health (SDoH) such as Socio Economic Status (SES), income and education on breast cancer survival, there was a serious lack of information about the effect of different level of these factors on breast cancer survival. So far, no meta-analysis has been conducted with this aim, but this gap was addressed by this meta-analysis. Methods: Main electronic databases such as PubMed, Web of Science, and Scopus were investigated up to January 2019. Epidemiological studies focusing on the association between SDoH and breast cancer were singled out. Q-test and I2 statistic were used to study the heterogeneity across studies. Begg's and Egger's tests were applied to explore the likelihood of the publication bias. The results were reported as hazard ratio (HR) with 95% confidence intervals (CI) through a random-effects model. Results: We identified 7,653 references and included 25 studies involving 1,497,881 participants. The HR estimate of breast cancer survival was 0.82 (0.67, 0.98) among high level of SES, 0.82 (0.70, 0.94) among high level of income and 0.72 (0.66, 0.78) among academic level of education. Conclusion: The SES, income, and education were associated with breast cancer survival, although the association was not very strong. However, there was a significant association between the levels of these factors and breast cancer survival.

Proteomics and theTrypanosoma bruceicytoskeleton: advances and opportunities

Parasitology ◽  
2012 ◽  
Vol 139 (9) ◽  
pp. 1168-1177 ◽  
Author(s):  
NEIL PORTMAN ◽  
KEITH GULL
Keyword(s):  
Life Cycle ◽  
Trypanosoma Brucei ◽  
Cell Biology ◽  
Molecular Composition ◽  
Etiological Agent ◽  
Sub Saharan Africa ◽  
Parasitic Disease ◽  
Cell Cycles ◽  
Sub Saharan ◽  
High Level

SUMMARYTrypanosoma bruceiis the etiological agent of devastating parasitic disease in humans and livestock in sub-saharan Africa. The pathogenicity and growth of the parasite are intimately linked to its shape and form. This is in turn derived from a highly ordered microtubule cytoskeleton that forms a tightly arrayed cage directly beneath the pellicular membrane and numerous other cytoskeletal structures such as the flagellum. The parasite undergoes extreme changes in cellular morphology during its life cycle and cell cycles which require a high level of integration and coordination of cytoskeletal processes. In this review we will discuss the role that proteomics techniques have had in advancing our understanding of the molecular composition of the cytoskeleton and its functions. We then consider future opportunities for the application of these techniques in terms of addressing some of the unanswered questions of trypanosome cytoskeletal cell biology with particular focus on the differences in the composition and organisation of the cytoskeleton through the trypanosome life-cycle.

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