Response of Pathogenic Vibrio Species to High Hydrostatic Pressure

ABSTRACT Vibrio parahaemolyticus ATCC 17802, Vibrio vulnificus ATCC 27562, Vibrio cholerae O:1 ATCC 14035, Vibrio cholerae non-O:1 ATCC 14547, Vibrio hollisae ATCC 33564, and Vibrio mimicus ATCC 33653 were treated with 200 to 300 MPa for 5 to 15 min at 25°C. High hydrostatic pressure inactivated all strains of pathogenicVibrio without triggering a viable but nonculturable (VBNC) state; however, cells already existing in a VBNC state appeared to possess greater pressure resistance.

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Occurrence of Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus in the Aquacultural Environments of Taiwan

Journal of Food Protection ◽

10.4315/0362-028x.jfp-14-405 ◽

2015 ◽

Vol 78 (5) ◽

pp. 969-976 ◽

Cited By ~ 10

Author(s):

YAO HSIEN TEY ◽

KOA-JEN JONG ◽

SHIN-YUAN FEN ◽

HIN-CHUNG WONG

Keyword(s):

Water Temperature ◽

Vibrio Cholerae ◽

Vibrio Parahaemolyticus ◽

Membrane Filtration ◽

Vibrio Vulnificus ◽

Pearson Correlation ◽

Isolation Frequency ◽

Freshwater Ponds ◽

Pathogenic Vibrio ◽

Southern Taiwan

The occurrence of Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae in a total of 72 samples from six aquaculture ponds for groupers, milk fish, and tilapia in southern Taiwan was examined by the membrane filtration and colony hybridization method. The halophilic V. parahaemolyticus was only recovered in seawater ponds, with a high isolation frequency of 86.1% and a mean density of 2.6 log CFU/g. V. cholerae was found in both the seawater and freshwater ponds but preferentially in freshwater ponds, with a frequency of 72.2% and a mean density of 1.65 log CFU/g. V. vulnificus was identified mainly in seawater ponds, with an isolation frequency of 27.8%. The density of V. parahaemolyticus in seawater ponds was positively related to water temperature (Pearson correlation coefficient, r = 0.555) and negatively related to salinity (r = −0.333). The density of V. cholerae in all six ponds was positively related to water temperature (r = 0.342) and negatively related to salinity (r = −0.432). Two putatively pathogenic tdh+ V. parahaemolyticus isolates (1.4% of the samples) and no ctx+ V. cholerae isolates were identified. The experimental results may facilitate assessments of the risk posed by these pathogenic Vibrio species in Taiwan, where aquaculture provides a large part of the seafood supply.

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Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

Molecular and Cellular Probes ◽

10.1016/j.mcp.2011.04.004 ◽

2011 ◽

Vol 25 (4) ◽

pp. 174-176 ◽

Cited By ~ 35

Author(s):

Hidemasa Izumiya ◽

Kazutoshi Matsumoto ◽

Shunsuke Yahiro ◽

Jiyoung Lee ◽

Masatomo Morita ◽

...

Keyword(s):

Multiplex Pcr ◽

Vibrio Cholerae ◽

Vibrio Parahaemolyticus ◽

Vibrio Vulnificus ◽

Pcr Assay ◽

Multiplex Pcr Assay ◽

Pathogenic Vibrio ◽

Vibrio Spp

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Inactivation of Vibrio parahaemolyticus and Vibrio vulnificus in oysters by high-hydrostatic pressure and mild heat

Food Microbiology ◽

10.1016/j.fm.2012.05.009 ◽

2012 ◽

Vol 32 (1) ◽

pp. 179-184 ◽

Cited By ~ 35

Author(s):

Mu Ye ◽

Yaoxin Huang ◽

Haiqiang Chen

Keyword(s):

Hydrostatic Pressure ◽

High Hydrostatic Pressure ◽

Vibrio Parahaemolyticus ◽

Vibrio Vulnificus ◽

Mild Heat

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Identification of novel genes involved in high hydrostatic pressure resistance of Escherichia coli

Food Microbiology ◽

10.1016/j.fm.2018.10.007 ◽

2019 ◽

Vol 78 ◽

pp. 171-178 ◽

Cited By ~ 4

Author(s):

Elisa Gayán ◽

Nele Rutten ◽

Jan Van Impe ◽

Chris W. Michiels ◽

Abram Aertsen

Keyword(s):

Escherichia Coli ◽

Hydrostatic Pressure ◽

High Hydrostatic Pressure ◽

Novel Genes ◽

Pressure Resistance

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Selectivity and Specificity of a Chromogenic Medium for Detecting Vibrio parahaemolyticus†

Journal of Food Protection ◽

10.4315/0362-028x-68.7.1454 ◽

2005 ◽

Vol 68 (7) ◽

pp. 1454-1456 ◽

Cited By ~ 20

Author(s):

YI-CHENG SU ◽

JINGYUN DUAN ◽

WEN-HSIN WU

Keyword(s):

Vibrio Parahaemolyticus ◽

Bile Salts ◽

Vibrio Vulnificus ◽

Enterobacter Cloacae ◽

Most Probable Number ◽

Chromogenic Medium ◽

Probable Number ◽

Vibrio Fluvialis ◽

Vibrio Mimicus ◽

Vibrio Spp

The thiosulfate–citrate–bile salts–sucrose agar (TCBS) used in the most-probable-number method for detecting Vibrio parahaemolyticus cannot differentiate growth of V. parahaemolyticus from Vibrio vulnificus or Vibrio mimicus. This study examined the selectivity and specificity of Bio-Chrome Vibrio medium (BCVM), a chromogenic medium that detects V. parahaemolyticus on the basis of the formation of distinct purple colonies on the medium. A panel consisting of 221 strains of bacteria, including 179 Vibrio spp. and 42 non-Vibrio spp., were examined for their ability to grow and produce colored colonies on BCVM. Growth of Salmonella, Shigella, Escherichia coli, Enterobacter cloacae, Yersinia enterocolitica, and Aeromonas was inhibited by both BCVM and TCBS. All 148 strains of V. parahaemolyticus grew on BCVM, and 145 of them produced purple colonies. The remaining 31 Vibrio spp., except one strain of Vibrio fluvialis, were either unable to grow or produced blue-green or white colonies on BCVM. Bio-Chrome Vibrio medium was capable of differentiating V. parahaemolyticus from other species, including V. vulnificus and V. mimicus. Further studies are needed to evaluate the sensitivity and specificity of BCVM for detecting V. parahaemolyticus in foods.

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