scholarly journals Identification of Another B-Cell Epitope in the Type-Specific Region of Equine Herpesvirus 4 Glycoprotein G

2005 ◽  
Vol 12 (1) ◽  
pp. 122-124 ◽  
Author(s):  
Ken Maeda ◽  
Fuminori Mizukoshi ◽  
Masataka Hamano ◽  
Kazushige Kai ◽  
Takashi Kondo ◽  
...  
Keyword(s):  
B Cell ◽  
Cell Epitope ◽  
Prototype Strain ◽  
Repeat Sequence ◽  
Specific Region ◽  
Enzyme Linked Immunosorbent Assay ◽  
Equine Herpesvirus ◽  
Repeat Sequences ◽  
Glycoprotein G ◽  
B Cell Epitope

ABSTRACT Recently, a novel 12-mer B-cell epitope, MKNNPIYSEGSL, in the type-specific region of equine herpesvirus 1 (EHV-1) glycoprotein G (gG) was identified and used as an antigen for enzyme-linked immunosorbent assay (Maeda et al., J. Clin. Microbiol. 42:1095-1098, 2004). Although our prototype strain, TH20p, possesses two repeat sequences containing the B-cell epitope, the EHV-4 NS80567 strain has two repeat sequences that are not identical. One repeat sequence stretch contained the B-cell epitope, while the other contained the 11-mer, MKNNP V YSESL (underlining indicates a different amino acid). In this study, heterogeneity of the type-specific region was compared among Japanese EHV-4 isolates. The 11-mer peptide, MKNNP V YSESL, specifically reacted with sera from horses naturally infected with EHV-4 but not with sera from horses experimentally infected with EHV-4 TH20p. The 11-mer peptide may be another B-cell epitope in the type-specific region.

Identification of human linear B-cell epitope sites on the envelope glycoproteins of Crimean-Congo haemorrhagic fever virus

2014 ◽  
Vol 143 (7) ◽  
pp. 1451-1456 ◽  
Author(s):  
D. GOEDHALS ◽  
J. T. PAWESKA ◽  
F. J. BURT
Keyword(s):  
B Cell ◽  
Cell Epitope ◽  
Fever Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Haemorrhagic Fever ◽  
Serum Samples ◽  
B Cell Epitope ◽  
Crimean Congo Haemorrhagic Fever ◽  
Peptide G ◽  
Linear B

SUMMARYA peptide library was used to screen for regions containing potential linear B-cell epitope sites in the glycoproteins and nucleoprotein of Crimean-Congo haemorrhagic fever virus (CCHFV) in an enzyme-linked immunosorbent assay (ELISA). The library consisted of 156 peptides, spanning the nucleoprotein and mature GN and GC proteins in a 19-mer with 9-mer overlap format. Using pooled serum samples from convalescent patients to screen the library, six peptides were identified as potential epitope sites. Further testing of these six peptides with individual patient sera identified two of these peptides as probable epitope sites, with peptide G1451–1469 reacting to 13/15 and peptide G1613–1631 to 14/15 human sera. These peptides are situated on the GC protein at amino acid positions 1451–1469 (relative to CCHFV isolate SPU103/97) (TCTGCYACSSGISCKVRIH) and 1613–1631 (FMFGWRILFCFKCCRRTRG). Identified peptides may have application in ELISA for diagnostic or serosurveillance purposes.

B-cell epitope mapping of YscF, a T3SS needle protein of Yersinia spp., with the panel of immune sera from vaccinated models

2020 ◽  
Author(s):  
Zaytsev Sergey ◽  
Motin Vladimir ◽  
Khizhnyakova Mariya ◽  
Feodorova Valentina Anatolievna ◽  
Elena Lyapina ◽  
...  
Keyword(s):  
B Cell ◽  
Epitope Mapping ◽  
Cell Epitope ◽  
B Cell Epitope ◽  
Needle Protein
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